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Animals
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Livestock and Poultry Genetics and Breeding Management
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Livestock and Poultry Genetics and Breeding Management

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Animal Genetics and Genomics".

Deadline for manuscript submissions: 31 December 2025 | Viewed by 1928

Special Issue Editor

Dr. Yuguo Yuan

E-Mail Website
Guest Editor
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China
Interests: modern biological breeding; cloning; gene knock; nano pharmaceuticals; goats; cats; mice; rabbits

Special Issue Information

Dear Colleagues,

​In light of issues like population expansion, disease outbreaks, and climate change, the rapidly evolving field of livestock and poultry genetics has become crucial to meeting the demands for sustainable food supply. A thorough grasp of the genetic and breeding mechanisms that improve the robustness, well-being, and output of animal populations is required to meet these difficulties. In order to choose exceptional animals and enhance characteristics like growth rates, illness resistance, and reproductive performance, effective breeding strategies incorporate genetic concepts.

The integration of novel techniques, such as genomic selection and biotechnologies, which enable more accurate and effective genetic alterations in cattle and poultry species, have dramatically altered traditional breeding processes. Furthermore, population genetics highlights the significance of preserving genetic variety within breeding programs, while the use of gene-editing technologies like CRISPR-Cas9 shows promise for improving productivity and disease resistance.

The goal of this Special Issue is to investigate a wide range of subjects, such as the effects of epigenetic factors on animal health and welfare and genetic improvement through selection procedures. To enhance our knowledge of these vital areas, we encourage researchers from a wide range of disciplines, such as genetics, animal breeding, veterinary medicine, and biotechnology, to submit original research articles, reviews, and brief communications. We encourage articles that examine the ecological and economic repercussions of breeding methods and their implications for livestock and poultry management.

Our goal with this Special Issue is to promote knowledge sharing that will eventually improve livestock and poultry breeding methods, supporting sustainable agricultural systems and animal populations' ability to adapt to changing environmental conditions. We anticipate your insightful contributions.

Dr. Yuguo Yuan
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Animals is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • animal genetics
  • breeding management
  • gene editing
  • genomic selection
  • livestock improvement
  • reproductive health
  • performance traits
  • cloning technologies
  • bioinformatics in breeding

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Further information on MDPI's Special Issue policies can be found here.

Published Papers (3 papers)

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Research

19 pages, 2901 KiB  
Article
SIRT5 Regulates Lipid Deposition in Goat Preadipocytes via PI3K-Akt and MAPK Signaling Pathways
by Haiyang Li, Wenli Yao, Changheng Yang, Wenyang Zhang, Yong Wang, Yaqiu Lin, Zhanyu Du, Changhui Zhang, Lian Huang, Ming Zhang, Huaigong Fan, Jiangjiang Zhu and Hua Xiang
Animals 2025, 15(7), 1072; https://doi.org/10.3390/ani15071072 - 7 Apr 2025
Viewed by 308
Abstract
Silent Information Regulator 5 (SIRT5) has been established as a crucial regulator of cellular alanylation modification. Furthermore, accumulating evidence suggests that SIRT5 plays a significant regulatory role in key metabolic pathways, including glycolysis, the tricarboxylic acid (TCA) cycle, and fatty acid [...] Read more.
Silent Information Regulator 5 (SIRT5) has been established as a crucial regulator of cellular alanylation modification. Furthermore, accumulating evidence suggests that SIRT5 plays a significant regulatory role in key metabolic pathways, including glycolysis, the tricarboxylic acid (TCA) cycle, and fatty acid oxidation, all of which are closely associated with cellular lipid metabolism. Despite these advancements, the specific role of SIRT5 in regulating intramuscular fat (IMF) deposition in goats, as well as the underlying molecular mechanisms, remains largely unexplored. In this study, we cloned the complete coding sequence of the goat SIRT5 gene and, through amino acid sequence alignment, demonstrated its closest phylogenetic relationship with sheep. Additionally, we characterized the higher expression of SIRT5 during the differentiation of goat intramuscular precursor adipocytes. The silencing of SIRT5 by siRNA-mediated knockdown significantly upregulated the expression of lipogenesis-related genes and enhanced lipid deposition in goat intramuscular preadipocytes. Concurrently, SIRT5 deficiency led to the inhibition of cell proliferation and a marked reduction in apoptosis. Interestingly, although overexpression of SIRT5 promoted cell proliferation, it did not significantly alter lipid deposition in goat intramuscular precursor adipocytes. RNA sequencing (RNA-seq) analysis identified a total of 106 differentially expressed genes (DEGs) following SIRT5 silencing in goat preadipocytes, predominantly involved in the Focal adhesion, HIF-1, PI3K-Akt, and MAPK signaling pathways by KEGG pathway enrichment analysis. Notably, we successfully reversed the phenotypic effects observed in SIRT5 knockdown goat precursor adipocytes by inhibiting the PI3K-Akt and MAPK signaling pathways using the AKT inhibitor LY294002 and the p38 MAPK pathway inhibitor PD169316, respectively. In conclusion, our findings demonstrated that SIRT5 may modulate intramuscular fat deposition in goats through PI3k-Akt and MAPK signaling pathways. These results expand the gene regulatory network associated with IMF formation and provide a theoretical foundation for improving meat quality by targeting IMF deposition. Full article
(This article belongs to the Special Issue Livestock and Poultry Genetics and Breeding Management)
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17 pages, 9444 KiB  
Article
Targeted Gene Knock-Out of Fel d1 in Fetal Fibroblasts Using CRISPR–Cas9: Implications for Cat Allergies
by Ling Li, Muhammad Farhab, Paing Oo Kyaw, Xiao-Xiao Xia, He-Qing Cai, Ting Zhang, Ming-Xing Cao, Jin-Gui Li and Yu-Guo Yuan
Animals 2025, 15(7), 927; https://doi.org/10.3390/ani15070927 - 24 Mar 2025
Viewed by 721
Abstract
Fel d1 is the most important allergen secreted by cats, which can trigger asthma in sensitive individuals. Our objective was to knock-out the Fel d1 gene in the fetal fibroblasts of cats through CRISPR–Cas9 technology with two sgRNAs and to determine the impact [...] Read more.
Fel d1 is the most important allergen secreted by cats, which can trigger asthma in sensitive individuals. Our objective was to knock-out the Fel d1 gene in the fetal fibroblasts of cats through CRISPR–Cas9 technology with two sgRNAs and to determine the impact of such mutations on the antigenicity of the Fel d1 protein. DNA samples from 38 domestic cats were collected and amplified by PCR to obtain the complete sequence of the Fel d1 gene. Throughout evolution, Fel d1 polypeptide chain 1(CH1) has proven to be much more conserved than Fel d1 polypeptide chain 2(CH2); therefore, we targeted CH2 and designed two single-guide RNAs (CH2-sgRNA-1 and CH2-sgRNA-2) for this region. Using these constructed sgRNAs, we performed gene knock-out in fetal fibroblasts, resulting in two mutations within the target gene. Following this, DNA was extracted and the target site product was cloned using TA cloning via PCR, and a single colony from this process was sequenced to analyze the physicochemical properties, antigenic sites, and three-dimensional structure of the mutated protein. The results revealed that there were 12 and 51 polymorphic loci (single-nucleotide polymorphisms, or SNPs) found in the CH1 and CH2 sequences, respectively, with most loci located in the GC-rich intron 2, while others were found in exon 2, intron 3, and exon 3. These SNPs guided sgRNA design by identifying conserved regions in the CH2 gene. The gene editing efficiency for the CH2 region, with this dual CRISPR system, was 40%, with 35% attributed to Type 1 mutation and 5% to Type 2 mutation. In conclusion, CH1 is significantly more conserved than CH2, and the antigenicity of the Fel d1 CH2 gene in domestic cats can be effectively reduced through CRISPR–Cas9 gene editing. Full article
(This article belongs to the Special Issue Livestock and Poultry Genetics and Breeding Management)
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19 pages, 4986 KiB  
Article
Analysis of the Transcriptional Control of Bcl11b in Chicken: IRF1 and GATA1 as Negative Regulators
by Lingling Qiu, Haojie Wang, Wenhao Li, Ting Yang, Hao Bai and Guobin Chang
Animals 2025, 15(5), 665; https://doi.org/10.3390/ani15050665 - 25 Feb 2025
Viewed by 412
Abstract
B-cell lymphoma/leukemia 11B (Bcl11b) plays roles in cell proliferation and apoptosis and holds a pivotal position within the immune system. Our previous studies have demonstrated that Bcl11b can promote cell apoptosis to curb ALV-J infection. To gain insights into the molecular mechanisms underlying [...] Read more.
B-cell lymphoma/leukemia 11B (Bcl11b) plays roles in cell proliferation and apoptosis and holds a pivotal position within the immune system. Our previous studies have demonstrated that Bcl11b can promote cell apoptosis to curb ALV-J infection. To gain insights into the molecular mechanisms underlying Bcl11b expression regulation in chickens, we constructed various truncated dual luciferase reporter vectors and analyzed the promoter region of Bcl11b. We employed promoter-binding TF profiling assay and the dual luciferase assay of site-directed mutagenesis and the expression level of interfering or overexpressing transcription factors were used to study their transcriptional regulation mechanism of chicken Bcl11b and functions in ALV-J infection. Our findings revealed core regulatory regions of the chicken Bcl11b promoter. By examining the −606~−363 bp region, we identified several transcription factors and their binding sites. Mutational and functional analysis further revealed interferon regulatory factor-1 (IRF1) and GATA-binding protein 1 (GATA1) as critical factors for the repression of chicken Bcl11b, thereby affecting cell apoptosis and ALV-J replication. Furthermore, DNA methylation analysis indicated that methylation may also contribute to changes in Bcl11b promoter activity. These findings offer valuable insights into the regulatory mechanisms of chicken Bcl11b and provide promising targets for molecular breeding and genetic improvement of disease resistance in chickens. Full article
(This article belongs to the Special Issue Livestock and Poultry Genetics and Breeding Management)
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