Recent Advances in Mammary Tumors in Companion Animals

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Companion Animals".

Deadline for manuscript submissions: 31 October 2026 | Viewed by 606

Special Issue Editors


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Departmento de Patologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Campus Pampulha, Av. Antônio Carlos, Belo Horizonte 6627, MG, Brazil
Interests: comparative mammary pathology; comparative pathology; translational research in cancer

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Department of Comparative Biomedicine and Food Science, University of Padua, Polo di Agripolis, Viale dell’Università 16, 35020 Legnaro, Italy
Interests: mammary pathology; breast cancer; tumor immunity
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Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, São Paulo State University – UNESP, Distrito de Rubião Júnior, s/n, Botucatu 18618 681, SP, Brazil
Interests: veterinary medicine; internal medicine; molecular oncology; comparative oncology
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Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, Italy
Interests: veterinary pathology; histopathology

Special Issue Information

Dear Colleagues,

Mammary tumors are among the most frequently diagnosed neoplastic diseases in companion animals and continue to pose significant clinical and diagnostic challenges. Over recent decades, key advances in veterinary oncology have improved our understanding of the biological behavior of these tumors, refined diagnostic methodologies, and expanded the range of available prognostic and predictive tools. Progress in molecular pathology, immunohistochemistry, and tumor microenvironment analysis has enhanced our ability to characterize tumor subtypes, evaluate patterns of invasion, and better estimate clinical outcomes.

At the same time, new technologies such as digital pathology, advanced imaging, and high-throughput analytical platforms are contributing to greater diagnostic accuracy and promoting more standardized criteria for histopathological evaluation. These developments support more precise decision-making and help guide the selection of appropriate therapeutic approaches.

This Special Issue will showcase recent scientific contributions that reflect the current state of knowledge on mammary tumors in companion animals. We invite original studies and comprehensive reviews addressing pathology, diagnosis, treatment, molecular features, classification updates, and emerging technologies. By integrating diverse perspectives from researchers and clinicians, this Special Issue will advance our understanding of mammary neoplasia and inspire further innovation in veterinary oncology. 

We look forward to receiving your contributions.

Dr. Geovanni Dantas Cassali
Prof. Dr. Valentina Zappulli
Dr. Carlos Eduardo Fonseca-Alves
Dr. Giovanni Pietro Burrai
Guest Editors

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Keywords

  • mammary tumors
  • companion animals
  • veterinary oncology
  • tumor pathology
  • molecular diagnostics
  • tumor microenvironment
  • histopathological evaluation
  • diagnostic advancements
  • prognostic markers
  • predictive markers

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Published Papers (1 paper)

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Research

19 pages, 5503 KB  
Article
A Novel Recombinant MAGE-B10-HSP110 Fusion Protein Enhances Innate and Adaptive Immune Responses in Mice: A Potential Vaccine Candidate for Canine Mammary Tumors
by Anucha Muenthaisong, Kanokwan Sangkakam, Pongpisid Koonyosying, Thanya Varinrak and Nattawooti Sthitmatee
Animals 2026, 16(9), 1374; https://doi.org/10.3390/ani16091374 - 29 Apr 2026
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Abstract
Melanoma-associated antigen (MAGE) is a promising immunotherapeutic target for cancer vaccines. Heat shock protein 110 (HSP110), expressed in various tumors, including canine mammary tumors, serves as a molecular marker. This study aimed to develop a recombinant fusion protein by linking HSP110 with MAGE-B10 [...] Read more.
Melanoma-associated antigen (MAGE) is a promising immunotherapeutic target for cancer vaccines. Heat shock protein 110 (HSP110), expressed in various tumors, including canine mammary tumors, serves as a molecular marker. This study aimed to develop a recombinant fusion protein by linking HSP110 with MAGE-B10 to target MAGE-B10-expressed tumors and assess immune response efficacy. The recombinant MAGE-B10-HSP110 (rMAGE-B10-HSP110) fusion protein was constructed, and separate recombinant MAGE-B10 (rMAGE-B10) and recombinant HSP110 (rHSP110) proteins were also prepared for comparison. Our study on mice is distributed across five treatment groups: the rMAGE-B10-HSP110 fusion protein, rMAGE-B10, rHSP110, a protein mixture, and a PBS control. Antibody responses specific to canine MAGE-B10 were measured using ELISA, while splenocyte activation, proliferation, and cytokine production were analyzed using flow cytometry. The results showed significantly higher antibody responses in mice immunized with the rMAGE-B10-HSP110 fusion protein compared to those receiving PBS or rHSP110 on days 7, 14, and 21. The proportion of CD3+ and CD4+ lymphocytes were significantly higher in these mice (p < 0.05). rMAGE-B10-HSP110 fusion protein immunization also resulted in increased CD69+ lymphocytes and higher IFN-γ levels in stimulated lymphocytes (p < 0.05). In conclusion, the rMAGE-B10-HSP110 fusion protein effectively stimulates both innate and adaptive immune responses. Further in vivo investigation is recommended. Full article
(This article belongs to the Special Issue Recent Advances in Mammary Tumors in Companion Animals)
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