Innovations in Animal Virus Detection and Therapeutics

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Veterinary Clinical Studies".

Deadline for manuscript submissions: 30 September 2026 | Viewed by 555

Special Issue Editors


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Guest Editor
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
Interests: mechanisms of viral infection and host immune response; RNA metabolism and immunity; novel detection techniques using fluorescence bioimaging method
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
Interests: dynamic analysis of viral infection mechanisms; regulation of metabolism and immune responses; novel vaccines and delivery systems; detection of animal-origin hazards; animal nutrition and health; single-molecule technologies
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Interests: novel diagnostic approaches; novel vaccine adjuvants

Special Issue Information

Dear Colleagues,

Animal viral diseases continue to cause substantial economic losses and pose serious health challenges to both livestock and companion animals, and even threaten human public health due to their zoonotic potential. The rapid development of innovative diagnostic technologies and therapeutic strategies is essential for early detection, effective disease control, and improved animal welfare.

This Special Issue, “Innovations in Animal Virus Detection and Therapeutics,” focuses on recent advances in animal virus detection and antiviral approaches. Topics include rapid and portable diagnostic platforms, novel immunoassay and nucleic acid-based detection methods, and the discovery of antiviral functional additives, small-molecule inhibitors, and immunomodulatory compounds. Studies related to virus–host interactions and mechanistic insights are also welcome.

We look forward to your valuable contributions.

Dr. Yanke Shan
Prof. Dr. Fei Liu
Dr. Jiahao Li
Guest Editors

Manuscript Submission Information

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Keywords

  • innovative diagnostic technologies
  • rapid detection
  • point-of-care diagnostics
  • antiviral agents
  • functional additives
  • immunomodulators
  • nucleic acid detection
  • animal viral diseases
  • virus–host interactions
  • zoonotic diseases

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Published Papers (1 paper)

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Research

15 pages, 3597 KB  
Article
A DPO-Enhanced Gold Nanoparticle-Assisted PCR Assay for Simultaneous Detection of Bovine Viral Diarrhea Virus, Astrovirus, and Rotavirus
by Dongjie Cai, Yulin Lin, Jie Chen, Bin Tian, Qing Liu, Xiaoping Ma, Jiabin Gao and Zhicai Zuo
Animals 2026, 16(6), 914; https://doi.org/10.3390/ani16060914 - 14 Mar 2026
Viewed by 319
Abstract
To develop a rapid detection tool for major bovine diarrheal viruses, Bovine viral diarrhea virus (BVDV), Bovine astrovirus (BAstV), and Bovine rotavirus (BRV), a gold nanoparticle-enhanced dual-priming oligonucleotide (DPO) multiplex PCR method was established. After optimization, the assay showed high sensitivity with detection [...] Read more.
To develop a rapid detection tool for major bovine diarrheal viruses, Bovine viral diarrhea virus (BVDV), Bovine astrovirus (BAstV), and Bovine rotavirus (BRV), a gold nanoparticle-enhanced dual-priming oligonucleotide (DPO) multiplex PCR method was established. After optimization, the assay showed high sensitivity with detection limits of 4.9 × 10−1, 2.72 × 102, and 1.88 × 103 copies/μL for BVDV, BAstV, and BRV, respectively, and demonstrated excellent specificity. In the validation using 963 clinical samples, the DPO-nanoPCR showed numerically higher detection rates for BVDV (5.50% vs. 4.36%) and BAstV (1.04% vs. 0.62%) compared to conventional PCR, while the detection rate for BRV was identical (0.93%). Statistical analysis indicated no significant difference between the two methods (p > 0.05), with an overall agreement rate exceeding 98.86%. The developed DPO-nanoPCR method provides a sensitive, specific, and efficient tool for the simultaneous surveillance and diagnosis of these key bovine enteric pathogens. Full article
(This article belongs to the Special Issue Innovations in Animal Virus Detection and Therapeutics)
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