Sustainable Bioprocess for Agricultural Waste Valorization

A special issue of Agriculture (ISSN 2077-0472). This special issue belongs to the section "Agricultural Technology".

Deadline for manuscript submissions: closed (15 March 2023) | Viewed by 1827

Special Issue Editors


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Guest Editor
Department of Microbiology, Bioprocess and Bioenergy Laboratory, Central University of Rajasthan, Rajasthan 305817, India
Interests: biomass valorization; biorefinery; bioenergy; value added products; biomass pretreatment
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Guest Editor
Assistant Professor, School of Biotechnology, Banaras Hindu University, Varanasi, India
Interests: biomass valorization; biorefinery; bioenergy; value added products; biomass pretreatment

Special Issue Information

Dear Colleagues,

The world’s demand for food, textiles, medicine, and many other essential needs is dependent on agriculture. The production of agricultural goods has increased manyfold, and to suffice the need of growing populations, it will once again greatly increase in the near future. However, this increase in the production of agricultural goods is also accompanied by a large amount of waste. The degradation of these huge amounts of agricultural residues is a gigantic problem worldwide. Nevertheless, humans have a principal responsibility to search for alternatives and turn a problem into an opportunity. Lignocellulosic-rich agricultural residues have been found to be an efficient source of biofuel, bio-oils, high-value compounds, and intermediate compounds for the commercially important biorefinery alternatives to petroleum-refinery-based chemicals. Thus, the valorization of agricultural biomass is a great opportunity and can be exploited for a greener future that supports both environmental conservation and meets the chemical and energy needs of mankind. The conversion of lignocellulosic biomass to biofuel and high-value compounds involves a series of processes such as pretreatment, hydrolysis, saccharification, and fermentation. Several technologies such as physical, chemical, physicochemical, and biological systems are developed for pretreatment and hydrolysis. Several physicochemical technologies are also developed for the direct conversion of biomass to high-value compounds. Similarly, the development of efficient hydrolytic enzymes and fermenting strains via the genetic engineering and system biology approach can lead to enhanced efficiency in saccharification and fermentation.

This Special Issue focuses on the development and assessment of these technologies and bioprocesses that can result in enhanced biomass valorization. This issue will provide a platform to include interdisciplinary studies embracing physical and chemical strategies for the enhanced recovery of high-value compounds and biochemical synthesis using agricultural residues as the starting material. Further approaches such as crop advancement for high cellulosic content and improved recombinant strains for enhanced saccharification and fermentation will also be included. Original research, opinions, and reviews are all welcome.

Prof. Dr. Pradeep Verma
Dr. Venkatesh Chaturvedi
Guest Editors

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Keywords

  • agricultural residues
  • valorization
  • pretreatment
  • genetic engineering
  • bioprocess development

Published Papers (1 paper)

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Research

19 pages, 17682 KiB  
Article
Isolation of an Acidophilic Cellulolytic Bacterial Strain and Its Cellulase Production Characteristics
by Shijia Zhang, Zhichao Wang, Jiong Shen, Xuantong Chen and Juan Zhang
Agriculture 2023, 13(7), 1290; https://doi.org/10.3390/agriculture13071290 - 23 Jun 2023
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Abstract
The aim of the study was to isolate and identify a highly efficient cellulolytic bacteria strain that can be used in acidic environments, and then investigate its cellulase production characteristics for the effective utilization of agricultural waste. For this purpose, we set a [...] Read more.
The aim of the study was to isolate and identify a highly efficient cellulolytic bacteria strain that can be used in acidic environments, and then investigate its cellulase production characteristics for the effective utilization of agricultural waste. For this purpose, we set a series of isolation and screening steps, 21 strains were isolated from soil, and an acidophilic strain labeled as B13-2 with high cellulase production was screened using the Gram’ iodine method and cellulase activity assay; it was identified as Raoultella terrigena. Lastly, the culture conditions such as incubation time, incubation temperature, pH, carbon sources, nitrogen sources, and inoculum size were optimized via single-factor experiments, and on this basis, the cellulase production of strain B13-2 was optimized using response surface methodology with cellulase activity as the optimization goal. The results of the response surface optimization showed that the optimum incubation time is 3.1 days, the optimum temperature is 29.9 °C, the optimum pH is 4.1, and the optimum inoculum size is 1.50%, the cellulase activity reached a maximum of 13.503 U/mL, which was about 140% higher than that before optimization. In particular, strain B13-2 had higher cellulase production when rice straws were used as the natural carbon source. Meanwhile, the SEM pictures demonstrated that the surface of the substrate rice straws in an acidic buffer with strain B13-2 was uneven, with larger holes than in the neutral buffer after incubation. It further proved that this strain has a stronger ability to degrade cellulose under acidic conditions. The B13-2 is a kind of acidophilic cellulolytic bacteria. Therefore, it has the potential to be developed into a silage additive agent and provides a high-quality strain resource for the high-value biotransformation of agricultural waste and lays a certain foundation for the sustainable development of agricultural cultivation. Full article
(This article belongs to the Special Issue Sustainable Bioprocess for Agricultural Waste Valorization)
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