Recent Advances In Vitro Regeneration and Micropropagation of Horticultural Crops

A special issue of Agriculture (ISSN 2077-0472). This special issue belongs to the section "Crop Production".

Deadline for manuscript submissions: 10 September 2026 | Viewed by 779

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Guest Editor
Centro Nacional de Recursos Genéticos-INIFAP, Boulevard de la Biodiversidad No. 400 Rancho las Cruces, Tepatitlán de Morelos 47600, Jalisco, Mexico
Interests: plant tissue culture; temporary immersion systems; nanoparticles; biostimulants
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Special Issue Information

Dear Colleagues,

In vitro propagation or micropropagation is a biotechnological technique that enables the increased production of propagules from various crops of agroalimentary, agroindustrial, ornamental, and medicinal interest. However, sophisticated in vitro regeneration techniques are required to achieve the mass propagation of these plant species within a reduced timeframe and limited space. To address this, innovative technological advancements are being employed, including temporary immersion systems, the use of cutting-edge substances such as biostimulants or nanoparticles, and the optimization of plant growth regulator (PGR) types and concentrations. Additionally, biotechnological techniques are being developed to enhance these plant propagation systems.

This Special Issue aims to unveil innovative strategies in plant micropropagation that are efficient and sustainable for obtaining horticultural crop propagules. It will also cover the biotechnological approaches that can be used to improve these processes. This Special Issue will include interdisciplinary and biotechnological studies focused on improving in vitro propagation processes and developing methodologies for conservation, food security, and sustainable agriculture. We invite the submission of original research articles, opinions, and reviews.

Dr. Marco Antonio Ramírez Mosqueda
Guest Editor

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Keywords

  • biotechnology
  • in vitro propagation
  • biostimulants
  • plant growth regulators

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Published Papers (1 paper)

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Review

21 pages, 7985 KB  
Review
A Bibliometric Analysis of Vanilla Micropropagation: Evolution, Collaborative Efforts and Future Pathways for Sustainability and Conservation
by Marco Vinicio Rodríguez-Deméneghi, Gael Francisco García-Merino, Noé Aguilar-Rivera, Fabiola Hernández-Ramírez and María Elena Montes-Ayala
Agriculture 2026, 16(9), 931; https://doi.org/10.3390/agriculture16090931 - 23 Apr 2026
Viewed by 394
Abstract
Vanilla (Vanilla planifolia Jacks. ex Andrews) is a tropical orchid of high economic value, with an annual production of 8000 to 10,000 t and a market exceeding 800 million USD in over 40 countries. In vitro propagation has strengthened the innovation, production, [...] Read more.
Vanilla (Vanilla planifolia Jacks. ex Andrews) is a tropical orchid of high economic value, with an annual production of 8000 to 10,000 t and a market exceeding 800 million USD in over 40 countries. In vitro propagation has strengthened the innovation, production, and conservation of this species. Bibliometrics, as a quantitative approach, systematically examines the patterns, dynamics, and evolutionary trends of scientific production. A systematic search was conducted in Scopus and Web of Science until December 2025, using the terms “vanilla” and “micropropagation”. A total of 53 documents were identified in Scopus (1997–2025) and 39 in Web of Science (2000–2025). The evaluated indicators included: year of publication, country of origin, language, areas, main categories, document typology, authorship, and keyword distribution. VOSviewer was used for keyword analysis to identify author collaboration networks and emerging trends. The years with the most information were 2024 and 2025, with Mexico and India standing out prominently. The main thematic areas were Agricultural and Biological Sciences, and the role of researcher Ramírez-Mosqueda was highlighted. The keywords with the highest correlation and impact were bioreactors, vanillin, and cryopreservation. This bibliometric study provides a comprehensive perspective on scientific production related to vanilla micropropagation. The results highlight the multidisciplinary nature of biotechnology applied to this crop, integrating contributions from various areas of knowledge for the benefit of the main actors in the value chain. Full article
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