Wild, Volume 3, Issue 1 (March 2026) – 14 articles

The Atlantic sharpnose (Rhizoprionodon terraenovae), blacknose (Carcharhinus acronotus), blacktip (Carcharhinus limbatus), and bonnethead (Sphyrna tiburo) sharks are commonly encountered large mobile consumers found in the estuaries along the western North Atlantic coast. The bulk of the dietary data for these species has been coarsely recorded at a broad taxonomic level (e.g., “teleost fish”). Here, we used DNA metabarcoding of fecal DNA collected using non-lethal cloacal swabs to identify the species of prey contributing to the diets of these shark species and measure the degree of trophic overlap. Samples were collected from 24 Atlantic sharpnose, 33 blacknose, six blacktip, and 17 bonnethead sharks in the summer of 2020. Based on previous dietary research on these shark species, we targeted teleost fishes and crustaceans using two previously published primer sets. From the 80 sharks sampled off the coast of North Carolina, we identified 38 prey taxa, with 82% classified to the species level and all assigned to at least the genus and family levels. The most common prey taxa found in the diet of the bonnethead was Atlantic blue crab (Callinectes sapidus; 44.75%, based on percent of occurrence) followed by penaeid shrimp (Penaeus spp.; 24.41%), mantis shrimp (Squilla empusa; 20.34%), and spot (Leiostomus xanthurus; 4.75%). Atlantic sharpnose and blacknose sharks had the largest Levin’s niche overlap, with both species relying on the same two most frequently consumed prey taxa: penaeid shrimp (Atlantic sharpnose: 33.33%, percent of occurrence, and blacknose: 34.78%) and spot (Atlantic sharpnose: 32.70% and blacknose: 22.32%). Bonnetheads and blacktips had the least amount of overlap between all shark species, where blacktips primarily consumed menhaden (Brevoortia spp.; 58.62%) and penaeid shrimp (26.44%). Our findings highlight the value of DNA metabarcoding in refining our understanding of predator diets, moving beyond broad taxonomic classifications to identify species-level prey associations and trophic interactions. As coastal habitats undergo increasing alteration due to anthropogenic impacts, such information is crucial for fisheries management, helping to identify key prey dependencies and anticipate potential ecosystem shifts. Full article

Protected area management plays a crucial role in conserving biodiversity, especially in areas where increasing demand for natural resources is associated with fast-growing human populations, such as West Africa. Investigating the perceptions of people with first-hand knowledge of protected area management provides important insights about biodiversity conservation, wildlife law enforcement, and human activities to inform adaptive management. Using 442 semi-structured interviews, we assessed the perceptions of park rangers and local residents in and around Fazao-Malfakassa National Park in Togo, West Africa, which was managed by the non-profit Franz Weber Foundation from 1990 to 2015, and since 2015 has been managed by the government of Togo. Both rangers and residents reported significant economic concerns following the park’s transfer from private to state management, with salary declines negatively affecting rangers and declines in community development projects and income-generating activities negatively affecting residents. Law enforcement capacity and resources also declined under state management, severely undermining the ability of rangers to curb illegal activities in the park, especially poaching and the destruction of trees to harvest wild honey and produce commercial charcoal. All rangers and most residents who had experience with both private and state park management preferred private management. There is an urgent need to increase surveillance and law enforcement capacity in the park to combat poaching and other illegal activities, and to engage local communities in the park’s long-term protection. To this end, rangers and residents are calling for change, and specifically recommend returning the park to competent private management to safeguard Togo’s last large refuge for nature and wildlife. Full article

The world is experiencing incredible biodiversity loss, including the decline of iconic species, such as elephants. The species faces an uncertain future due to habitat loss, human-elephant conflict, poaching and climate change, reminding us of the urgency of acting on a local and global scale. Art has historically been a powerful medium for expressing ideas and emotions, fostering deep connections for people. Therefore, this paper explores the impact of the sharing of scientific content through dance on conservation values in young people. Understanding conservation needs and analyzing what drives people to gain an emotional affinity towards the environment has shown the potential to support and innovate traditional education. The work presented here uses a dance piece, performed through a choreographic process with dance students, to educate an audience about the importance and behavior of the African savannah elephant and the threats to its survival. Our findings indicated differences between the level of knowledge and opinion of the audience throughout the different phases of the methodology explored here, revealing that dance (and artistic) education can provide knowledge and stimulate more empathy for species conservation. Full article

There is little question that the distribution and numbers of large mammals have been altered by human activities. In North America, native ungulates have declined substantially since the mid-1800s. There is no baseline, however, against which to measure the magnitude of changes aside from anecdotal accounts or published information derived from a single location at a single point in time and extrapolated to derive a continent-wide population estimate. One example is bighorn sheep (Ovis canadensis), which were estimated to number from 1.5 to 2.0 million individuals in 1850. The literature is replete with references to those numbers, yet the derivation of that ‘estimate’ has not been formally questioned; bighorn sheep, which are endemic to North America, currently number approximately 85,000 individuals. We combined basic math and logic to develop a simple model of population density at differing scales and examined results reductio ad absurdum to question the estimate of 1.5 million bighorn sheep and the magnitude of the hypothesized decline that followed. We evaluated the plausibility of bighorn sheep population densities that must have existed at four spatial scales if there were ≥1.5 million individuals and argue that such numbers likely never existed. Continued use of 1.5 million bighorn sheep as a baseline against which to assess the magnitude of anthropogenic impacts is unwise, inappropriate, and apt to become problematic in the context of the conservation of habitat, populations, or restoration to historical ranges. Credibility matters in science, management, and conservation. Full article

Roads impact medium- and large-sized mammal populations through both collisions and barrier effects. This study examined how landscape characteristics influence roadkill occurrences along the Dom Pedro I highway (SP-065), located in the Cantareira-Mantiqueira Corridor, São Paulo State, Brazil. The SP-065 crosses important remnants of the Brazilian Atlantic Forest, a global hotspot for biodiversity. Roadkill records were obtained from the Environmental Company of the State, and land use data were extracted from the MapBiomas platform. We analyzed seven landscape variables (in percentage): native forest, pasture, agriculture, forestry, urban areas, mosaic of uses, and water bodies, considering multiple spatial scales. Mammal species were grouped functionally by home range size and tolerance to anthropogenic environments. In total, 1418 roadkills of 24 species were recorded, including eight threatened species. Capybaras (Hydrochoerus hydrochaeris) were the most frequently killed species. Generalized linear models showed that, for Group G1 (small home range, common in human-modified areas), roadkills were positively associated with native forest and pasture, and negatively with mosaic landscapes. For Group G3 (large home range, tolerant to anthropogenic areas), agriculture had a positive effect, especially within a 3000 m radius. For Group G5 (capybara), roadkills increased with pasture and agriculture, while mosaic uses had a negative effect. Since pasture and agriculture were frequently linked to higher roadkill rates, environmental impact assessments should consider these land-use types when planning mitigation actions. Ultimately, responsibility for roadkill extends beyond highway managers to rural landowners and local governments, as land-use patterns around roads strongly influence mammal movement and mortality. Full article

The feral goat, Capra hircus, is an important example of the feralisation of a domesticated species. The intentional abandonment of herds, escapes, rural depopulation and abandonment of livestock and traditional agriculture have led original domestic goats to freely occupy different habitats. The aim of this study was to review the current global knowledge of the feral goat, identify its gaps and provide insights into possible future research. The majority of publications focus on islands in the Mediterranean Basin and Oceania. The information is particularly encompassed in terms of population distribution, habitat use, monitoring methods, potential environmental impacts and management strategies. The feral goat is considered one of the 100 worst invasive species, especially on islands; thus, there are abundant studies supporting their eradication fundamentally due to their negative impact on vegetation. However, their role as a food supply for endangered fauna taxa is not sufficiently understood. Oceania is the main advocate for feral goat eradication, along with meat production exploitation. The most widespread management approach is eradication, notably favoured in insular ecosystems. There is still a lack of updated numerical data available on the efficacy of eradication interventions. The insights obtained from deeper research could benefit management and conservation decision-making. Full article

We present WM-Classroom v1.0, a pedagogical multi-species agent-based model (ABM) designed for educational purposes in predator–prey–harvest systems. The model embeds a predator, two prey breeds, and human harvesters on a homogeneous 50 × 50 grid with weekly time steps, implementing random movement, abstract energetics, prey consumption, reproduction, legal harvest with species-specific cut-offs and seasons, optional predator control, and a poaching switch. After basic technical checks (energetic calibration, prey composition, herbivore viability), we explore the consistency of the model under illustrative scenarios including no hunting, single-prey harvest, hunter-density and season-length gradients, predator removal, and poaching. In the no-hunting baseline (n = 100), mean end-of-run abundances were 22 deer, 159 boar, and 45 wolves, with limited extinction events. Deer-only harvest often drove deer to very low end-of-run counts (mean 1–16) with extinctions in 2–7/10 replicates across cut-offs, whereas boar-only harvest showed higher persistence (mean 11–74) and boar extinctions occurred only at the lowest cut-off (3/10). Increasing hunter numbers or season length depressed prey and could indirectly reduce wolves via prey depletion. Legal predator control reduced predators as designed, while poaching had little effect under the implemented rules. Because interaction and prey-choice rules are simplified for transparency, outcomes should be interpreted as conditional on model assumptions. WM-Classroom v1.0 provides a didactic sandbox for courses, professional training, and outreach, with extensions (habitat heterogeneity, age/sex structure, probabilistic diet/kill success, and calibration/validation) outlined for future versions. Full article

The Mediterranean pond turtle (Mauremys leprosa) is a native semi-aquatic species of the Iberian Peninsula, southern France, and North Africa, widely distributed across Mediterranean aquatic systems. Within these environments, M. leprosa inhabits a mosaic of lotic (flowing) and lentic (still) habitats, whose structure and connectivity may influence its spatial use, behavior, and ontogenetic development. How morphometry and age-class distribution differ between these habitat types, however, remains unclear. This study analyzed morphometric differences among individuals from both habitat types to explore potential ontogenic habitat preferences. Lotic habitats were primarily used for dispersal and breeding by adults, while lentic habitats served as foraging and residency areas for juveniles. Morphometric differences between habitat types support this functional differentiation. These findings highlight the ecological importance of habitat heterogeneity and underscore the need to preserve both habitat types to support the full life cycle of M. leprosa in Mediterranean ecosystems and suggest potential evolutionary and ecological consequences of habitat-related morphometric and demographic variation. Full article

Rewilding and ecosystem restoration approaches have focused strongly on the restoration of wildlife/biodiversity. However, the Convention on Biological Diversity defines an ecosystem as “a dynamic complex of plant, animal and micro-organism communities and their non-living environment interacting as a functional unit”. It follows, therefore, that ecosystem restoration must involve the restoration of both the living and the non-living components of the environment, including their dynamic interactions. This paper defines other aspects of the environment, including nature and natural capital. These involve both biotic and abiotic components, so “nature” should not be used as a synonym for wildlife/biodiversity. After describing how geodiversity is important in ecosystem functioning, several examples are presented of how geomorphology is a crucial aspect of rewilding or landscape/ecosystem restoration. By pursuing this integrated approach to biotic and abiotic restoration, stronger, more-resilient ecosystems can be achieved. Full article

Predation of sea turtles by jaguars (Panthera onca) in the Santa Rosa National Park (SRNP) has been well documented over the past decade. However, the factors that influence jaguar feeding behavior, including environmental factors or characteristics of the beaches and the adjacent forest, are poorly known. This study aimed to identify the relationship between vegetation density and human activity on the distribution of feeding sites of jaguar on sea turtles at nesting beaches in Santa Rosa National Park, Costa Rica. We sampled three beaches (Naranjo, Nancite, and Colorada), where we identified and registered sea turtle carcasses preyed on by jaguars between June and November 2019. Through systematic searches of the forest adjacent to the beach, we documented the species, geographic coordinates, carcass length and width, vegetation cover at the carcass site, and the average vegetation coverage corresponding to the date and beach of each sea turtle carcass. In total, we recorded 338 sea turtle carcasses preyed on by jaguars, 156 at Naranjo beach, 103 at Nancite beach, and 89 at Colorada beach. The beach with the highest average density of carcasses was Colorada (8.7 (SD = 5.42)/ha), followed by Nancite (6.06 (SD = 5.58)/ha) and Naranjo (2.64 (SD = 1.79)/ha). The dragging distance from the beach line to sea turtle carcasses was best explained by the interaction of nesting beach and canopy cover at the carcass. Our canopy cover results may reflect that jaguars select sites that better hide their prey, in the same way that green turtles (Chelonia mydas) usually prefer areas with good coverage to nest in, contrasting to the nesting behavior of olive ridleys (Lepidochelys olivacea). On beaches, higher concentrations were observed where there was less human presence and this may reflect both turtle nesting and jaguar predation activity. Full article

Understanding the spatial structure of large mammals is critical for conservation planning, especially under increasing habitat fragmentation. This study applies an integrated spatial analysis combining the DBSCAN density-based clustering algorithm and the α-hull method to delineate non-convex geographic ranges of the maned wolf (Chrysocyon brachyurus) across South America. Using 454 occurrence records filtered for ecological reliability, we identified 11 geographically isolated α-populations distributed across five countries and multiple biomes, including the Cerrado, Chaco, and Atlantic Forest. The sensitivity analysis of the α parameter demonstrated that values below 2 failed to generate viable polygons, while α = 2 provided the best balance between geometric detail and ecological plausibility. Our results reveal a highly fragmented distribution, with α-populations varying in area from 43,077 km² to 566,154.7 km² and separated by distances up to 994.755 km. Smaller and peripheral α-populations are likely more vulnerable to stochastic processes, genetic drift, and inbreeding, while larger clusters remain functionally isolated due to anthropogenic barriers. We propose the concept of ‘α-population’ as an operational unit to describe geographically and functionally isolated groups identified through combined spatial clustering and non-convex hull analysis. This approach offers a reproducible and biologically meaningful framework for refining range estimates, identifying conservation units, and guiding targeted management actions. Overall, integrating α-hulls with density-based clustering improves our understanding of the species’ fragmented spatial structure and supports evidence-based conservation strategies aimed at maintaining habitat connectivity and long-term viability of C. brachyurus populations. Full article

Ruminant herbivores interact dynamically with their food resources, especially in deserts, where plant availability fluctuates sharply across seasons. We evaluated how seasonal food availability and the nutritional traits of preferred plants shape the diet and macronutrient niche of a desert mule deer (Odocoileus hemionus) population in the buffer zone of the Médanos de Samalayuca protected area, northern Mexico. From 2021 to 2022 we quantified seasonal food plant availability and characterized mule deer diet using microhistological fecal analysis and the nutrient content by right-angled mixture triangles. Mule deer diets were consistently low in diversity and dominated by grass, but preferred species shifted seasonally among shrubs, succulents, and grasses. Deer strongly selected some plant species that were scarce in the landscape, particularly during the cold-dry season. Preferred plants generally had high carbohydrate and variable protein contents, with the highest protein proportions in the temperate-dry season. Mixture triangles showed a narrow, carbohydrate-biased macronutrient niche, with the broadest range of nutrient mixtures in the temperate-dry season. Overall, our results support an opportunistic foraging strategy in which mule deer consume what is seasonally available while selectively using key plant species to maintain a relatively constant nutritional balance under limited and variable food resources. Full article

Understanding seasonal patterns in mammals’ diversity and spatial distribution is vital for effective biodiversity conservation. This study examines seasonal variations in mammal diversity and distribution in the Pénéssoulou Classified Forest (northwest Benin). We hypothesized that (i) species richness and abundance are highest during the rainy season due to greater water and food availability; and (ii) small mammals exhibit spatial clustering, whereas large mammals are more spatially dispersed. We carried out negative binomial regression to analyze the effects of season, vegetation type, and proximity to waterholes on species richness. Spatial patterns were analyzed using Moran’s I index across size categories. We found lower species richness in the dry season (β = −0.812 ± 0.375, p = 0.030), which supports our hypothesis and is likely driven by increased resource availability. Contrary to our expectation, small mammals tended to be more dispersed during the rainy season, while larger mammals showed greater dispersion in the dry season. Significantly lower species richness was observed in gallery forests (β = −2.794 ± 1.144, p = 0.015), probably due to seasonal flooding, which limits access to resources. Our findings underscore the importance of incorporating seasonal and habitat-specific variables into wildlife monitoring and management. Full article

Understanding how land tenure and socio-economic pressures shape landscape transformation is critical for sustainable management in biodiversity-rich regions. This study examines three decades (1987–2017) of land use and land cover (LU&LC) change in the Ngerenyi area of the Taita Hills, Kenya, by integrating multispectral Landsat analysis with household survey data. Harmonized pre-processing and supervised classification of four LU&LC classes, agriculture, built-up areas, high-canopy vegetation, and low-canopy vegetation, achieved overall accuracies above 80% and Kappa values exceeding 0.75. Transition modeling using the Minimum Information Loss Transition Estimation (MILTE) approach, combined with net-versus-swap metrics, revealed persistent decline and fragmentation of high-canopy vegetation, cyclical transitions between agriculture and low-canopy vegetation, and the near-irreversible expansion of built-up areas. Low-canopy vegetation exhibited the highest dynamism, reflecting both degradation from canopy loss and natural regeneration from fallowed cropland. Household surveys (n = 141) identified agricultural expansion, charcoal production, fuelwood extraction, and population growth as the dominant perceived drivers, with significant variation across tenure categories. The population in Taita Taveta County increased from 205,334 in 2009 to 340,671 in 2019, reinforcing documented pressures on land resources and woody biomass. As part of the Eastern Arc biodiversity hotspot, the landscape’s diminishing high-canopy patches underscore the importance of conserving undisturbed vegetation remnants as ecological baselines and biodiversity refuges. The findings highlight the need for tenure-sensitive, landscape-scale planning that integrates private landowners, regulates subdivision, promotes agroforestry and alternative energy options, and safeguards remaining high-canopy vegetation to enhance ecological resilience while supporting local livelihoods. Full article