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Zoonotic Dis., Volume 5, Issue 3 (September 2025) – 3 articles

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12 pages, 985 KiB  
Article
Reverse Transcription Loop-Mediated Isothermal Amplification Assay Using Samples Directly: Point-of-Care Detection of Severe Fever with Thrombocytopenia Syndrome Virus
by Marla Anggita, Kyoko Hayashida, Miyuka Nishizato, Hiroshi Shimoda and Daisuke Hayasaka
Zoonotic Dis. 2025, 5(3), 19; https://doi.org/10.3390/zoonoticdis5030019 (registering DOI) - 11 Jul 2025
Abstract
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick-borne disease caused by the SFTS virus (SFTSV). A rapid and cost-effective point-of-care testing detection system is important for the early diagnosis of SFTS. Herein, we developed a ready-to-use dried reverse transcription loop-mediated isothermal [...] Read more.
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick-borne disease caused by the SFTS virus (SFTSV). A rapid and cost-effective point-of-care testing detection system is important for the early diagnosis of SFTS. Herein, we developed a ready-to-use dried reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the direct detection of SFTSV in clinical samples. The assay enables simple, RNA-extraction-free detection using heat-treated serum or plasma, followed by a 30 min incubation at 65 °C. The results are visually interpreted through the color emitted, which can be observed under LED light. The established assay demonstrated detection sensitivity for SFTSV at 104 copies/µL and was effective in identifying infections in cats. Despite being less sensitive than real-time RT-PCR, this dried RT-LAMP method offers a rapid, cost-effective alternative suitable for point-of-care use, particularly in remote or resource-limited settings. The simplified workflow and visual readout make it a practical tool for the early detection and daily surveillance of SFTSV in animals. Full article
10 pages, 244 KiB  
Editorial
Drivers of Zoonotic Viral Spillover: Understanding Pathways to the Next Pandemic
by Jonathon D. Gass, Jr.
Zoonotic Dis. 2025, 5(3), 18; https://doi.org/10.3390/zoonoticdis5030018 - 7 Jul 2025
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Abstract
In the wake of the COVID-19 pandemic and amid growing concerns regarding viral threats such as avian influenza, Mpox, and HKU5 bat coronaviruses, the phenomenon of viral zoonotic spillover, when viruses leap from circulation in non-human animals to humans, has garnered unprecedented global [...] Read more.
In the wake of the COVID-19 pandemic and amid growing concerns regarding viral threats such as avian influenza, Mpox, and HKU5 bat coronaviruses, the phenomenon of viral zoonotic spillover, when viruses leap from circulation in non-human animals to humans, has garnered unprecedented global attention [...] Full article
(This article belongs to the Special Issue Viral Zoonotic Diseases and Spillover Risks)
20 pages, 2091 KiB  
Article
Zoonoses Under the Radar: What Do High School Students Really Know?
by Ronaldo Alves Pereira-Junior, Isabella Marques Nascimento, Maria Eduarda Barbiéri-Machado and Lorena Lopes Ferreira
Zoonotic Dis. 2025, 5(3), 17; https://doi.org/10.3390/zoonoticdis5030017 - 1 Jul 2025
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Abstract
Zoonotic diseases are a persistent public health concern, especially in low- and middle-income countries like Brazil. This cross-sectional study evaluated the knowledge and perceptions of 132 high school students (70 public and 62 private) in Goiânia, Brazil, regarding zoonoses, using a structured questionnaire. [...] Read more.
Zoonotic diseases are a persistent public health concern, especially in low- and middle-income countries like Brazil. This cross-sectional study evaluated the knowledge and perceptions of 132 high school students (70 public and 62 private) in Goiânia, Brazil, regarding zoonoses, using a structured questionnaire. Statistical analyses (Chi-square and Fisher’s exact tests) revealed significant differences (p < 0.05) between public and private school students in knowledge levels, pet care practices, and the awareness of zoonotic risks. While pet ownership was common in both groups, only 53% of private and 21% of public school students correctly defined “zoonosis.” Rabies, taeniasis, leptospirosis, tuberculosis, cysticercosis, cutaneous larva migrans, and leishmaniasis were the most frequently cited diseases, with private school students demonstrating greater recognition across all categories. However, most participants lacked detailed knowledge about transmission routes and prevention. Misconceptions—such as zoonoses affecting only low-income populations—were also identified. Preventive actions like sanitation, public education, and vaccination were commonly suggested but not consistently linked to zoonoses. These findings highlight critical educational gaps and emphasize the need to incorporate One Health principles into school curricula to improve youth understanding and support public health strategies for zoonosis prevention. Full article
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