Study of the Biodiversity and Antibiotic Activity of Microorganisms Isolated from the Nasal Mucosa of the Mangalica Pig †
by
, and
Anna A. Baranova
1,2,*, 
Vera A. Alferova
1,2,
Yuliya V. Zakalyukina
3
and
Vladimir A. Korshun
1,2 1
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia
2
Gause Institute of New Antibiotics, Russian Academy of Sciences, Moscow 119021, Russia
3
Department of Soil Science, Lomonosov Moscow State University, Moscow 119234, Russia
*
Author to whom correspondence should be addressed.
†
Presented at the 2nd International Electronic Conference on Antibiotics—Drugs for Superbugs: Antibiotic Discovery, Modes of Action and Mechanisms of Resistance, 15–30 June 2022; Available online: https://eca2022.sciforum.net/ .
Med. Sci. Forum 2022, 12(1), 23; https://doi.org/10.3390/eca2022-12698
Published: 15 June 2022
(This article belongs to the Proceedings of The 2nd International Electronic Conference on Antibiotics—Drugs for Superbugs: Antibiotic Discovery, Modes of Action and Mechanisms of Resistance)
Abstract
:Mammals ubiquitously interact with environmental and symbiotic microbes, and the effects of these interactions on animal physiology are currently the subject of intense interest. Symbionts of mammals are interesting from the point of view of their adaptation to their environment, including the emergence of defense mechanisms against competitors, antibiotic activity, the absence of a cytotoxic effect on host cells, competition for mucosal attachment sites and food sources. Microflora and mucous membranes closely interact, exerting a mutual influence on the health of a mammal. In this study, samples of the nasal mucosa of Mangalica pigs (a Hungarian breed of domestic pig) were studied. The samples were taken in January 2022 from the noses of one-year-old domestic pigs that had not been seriously ill or treated with antibiotics. Cultures of microorganisms were isolated on SMS agar (0.125 g/L of casein digest, 0.1 g/L of potato starch, 1 g/L of casamino acids, 15 g/L of bacto agar («HiMedia Laboratories Pvt. Ltd. Mumbai, India»)) and Mueller–Hinton agar («HiMedia Laboratories Pvt. Ltd. Mumbai, India»). The biodiversity of microorganisms and their antibiotic and antagonistic activity were studied. The resistance of the obtained cultures to the main human antibiotics was also tested. Antimicrobial activity of the isolated strains against a number of microorganisms was evaluated. The following test strains were used: a fungus (Aspergillus niger INA 00760), a yeast (Candida albicans CBS 8836), Gram-positive bacteria (Bacillus subtilis ATCC 6633, Staphylococcus aureus 29213, Staphylococcus aureus ATCC 25293, Staphylococcus aureus ATCC 43300 (MRSA), Staphylococcus epidermidis ATCC 14990, Enterococcus faecalis ATCC 29212) and Gram-negative bacteria (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853). As a result, the strain SM-11 with pronounced antimicrobial properties was selected for further investigation. The active strain was found to produce bright green fluorescent pigment.
Supplementary Materials
The following are available online at https://www.mdpi.com/article/10.3390/eca2022-12698/s1.
Author Contributions
Conceptualization, A.A.B. and V.A.A.; methodology, A.A.B., V.A.A. and Y.V.Z.; investigation, A.A.B., V.A.A., Y.V.Z. and V.A.K. All authors have read and agreed to the published version of the manuscript.
Funding
This research received no external funding.
Institutional Review Board Statement
Not applicable.
Informed Consent Statement
Not applicable.
Conflicts of Interest
The authors declare no conflict of interest.
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MDPI and ACS Style
Baranova, A.A.; Alferova, V.A.; Zakalyukina, Y.V.; Korshun, V.A. Study of the Biodiversity and Antibiotic Activity of Microorganisms Isolated from the Nasal Mucosa of the Mangalica Pig. Med. Sci. Forum 2022, 12, 23. https://doi.org/10.3390/eca2022-12698
AMA Style
Baranova AA, Alferova VA, Zakalyukina YV, Korshun VA. Study of the Biodiversity and Antibiotic Activity of Microorganisms Isolated from the Nasal Mucosa of the Mangalica Pig. Medical Sciences Forum. 2022; 12(1):23. https://doi.org/10.3390/eca2022-12698
Chicago/Turabian StyleBaranova, Anna A., Vera A. Alferova, Yuliya V. Zakalyukina, and Vladimir A. Korshun. 2022. "Study of the Biodiversity and Antibiotic Activity of Microorganisms Isolated from the Nasal Mucosa of the Mangalica Pig" Medical Sciences Forum 12, no. 1: 23. https://doi.org/10.3390/eca2022-12698
