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Biol. Life Sci. Forum, 2023, ENVABIO100

The 1st International Conference of the Red CYTED ENVABIO100 “Obtaining 100% Natural Biodegradable Films for the Food Industry”

San Lorenzo, Paraguay | 14–16 November 2022

Volume Editors:
Germán Ayala, Federal University of Santa Catarina, Brazil
Mary Lopretti, Universidad de la República, Uruguay
Maria-Filomena Barreiro, CIMO-SusTEC, Instituto Politécnico de Bragança, Portugal
José Vega-Baudrit, LANOTEC-CeNAT-CONARE, Costa Rica
Jairo Perilla, Universidad Nacional de Colombia, Colombia
Shirley Duarte, Universidad Nacional de Asunción, Paraguay

Number of Papers: 13

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Cover Story (view full-size image): The 1st International Conference of the Red CYTED ENVABIO100 “Obtaining 100% natural biodegradable films for the food industry” was held on 14–16 November 2022 in the National [...] Read more.
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2 pages, 660 KiB  
Editorial
Preface to the 1st International Conference of the Red CYTED ENVABIO100 “Obtaining 100% Natural Biodegradable Films for the Food Industry”
by Germán Ayala, Maria-Filomena Barreiro, Shirley Duarte, Mary Lopretti, Jairo E. Perilla and José Vega-Baudrit
Biol. Life Sci. Forum 2023, 28(1), 7; https://doi.org/10.3390/blsf2023028007 - 7 Nov 2023
Viewed by 735
Abstract
In submitting these conference proceedings to Biology and Life Sciences Forum, the Volume Editors certify to the publisher that all the papers published in this volume have been subjected to peer review administered by the Volume Editors [...] Full article
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Other

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7 pages, 1768 KiB  
Proceeding Paper
Evaluation of Agro-Industrial Carbon and Energy Sources for Lactobacillus plantarum M8 Growth
by José Escurra, Francisco P. Ferreira, Tomás R. López and Walter J. Sandoval-Espinola
Biol. Life Sci. Forum 2023, 28(1), 1; https://doi.org/10.3390/blsf2023028001 - 6 Nov 2023
Viewed by 991
Abstract
Lactic acid is a compound used industrially due to its properties. There are two methods for its production: chemical synthesis and microbial fermentation. In microbial fermentation, food industry waste can be used as a substrate, providing a route towards achieving a circular economy. [...] Read more.
Lactic acid is a compound used industrially due to its properties. There are two methods for its production: chemical synthesis and microbial fermentation. In microbial fermentation, food industry waste can be used as a substrate, providing a route towards achieving a circular economy. Thus, this study evaluated different substrates for Lactobacillus plantarum growth, a lactic acid producer, such as molasses, whey, glucose, and saccharose, either alone or supplemented with additional nutrients. Bacterial growth parameters were assessed using OD620 measurement. It was shown that whey supplemented with yeast extract supported the best growth, allowing a μmax = 0.63 h−1. Full article
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6 pages, 722 KiB  
Proceeding Paper
Nanocellulose and Its Application in the Food Industry
by Talita Szlapak Franco, Graciela Boltzon de Muniz, María Guadalupe Lomelí-Ramírez, Belkis Sulbarán Rangel, Rosa María Jiménez-Amezcua, Eduardo Mendizábal Mijares, Salvador García-Enríquez and Maite Rentería-Urquiza
Biol. Life Sci. Forum 2023, 28(1), 2; https://doi.org/10.3390/blsf2023028002 - 6 Nov 2023
Viewed by 2891
Abstract
This work presents a review related to the obtainment of cellulose from different structures in agro-industrial residues, both for application in the food industry and for the reinforcement of other materials. Cellulose nanofibers are produced by the heart of palm (Bactris gasipaes [...] Read more.
This work presents a review related to the obtainment of cellulose from different structures in agro-industrial residues, both for application in the food industry and for the reinforcement of other materials. Cellulose nanofibers are produced by the heart of palm (Bactris gasipaes) industry in Brazil and are used as a stabilizer in avocado oil emulsions; conversely, cellulose nanocrystals are produced in waste from the tequila industry (Agave tequilana Weber var. Azul) in Jalisco, Mexico, and are used for reinforcement applications. Full article
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6 pages, 868 KiB  
Proceeding Paper
Study of the Mechanical Properties of Gels Formulated with Pectin from Orange Peel
by Nicolás Mauricio Bogdanoff, Camilo J. Orrabalis, Wilson Daniel Caicedo Chacon and Germán Ayala Valencia
Biol. Life Sci. Forum 2023, 28(1), 3; https://doi.org/10.3390/blsf2023028003 - 6 Nov 2023
Viewed by 850
Abstract
Pectin is a polysaccharide that is known for its gelling properties and its applications in the pharmaceutical industry. This can be divided into two structural groups, high methoxyl pectins (HMP) and low methoxyl pectins (LMP). Currently, there is little information on the properties [...] Read more.
Pectin is a polysaccharide that is known for its gelling properties and its applications in the pharmaceutical industry. This can be divided into two structural groups, high methoxyl pectins (HMP) and low methoxyl pectins (LMP). Currently, there is little information on the properties of the orange pectin in which LPM predominates. The aim of this study was therefore to investigate the mechanical properties of gels produced with pectin isolated from orange peels. The results showed similar values to those found in the literature, except for hardness. The gels produced from the pectin could be used in the industry, the formulation varying depending on the application. Full article
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7 pages, 981 KiB  
Proceeding Paper
Preliminary Modeling Study of a Tape Casting System for Thermoplastic Starch Film Forming
by Liliana Ávila-Martín, Diana Katherine Guzmán Silva, Jairo E. Perilla and Cristian Camilo Villa Zabala
Biol. Life Sci. Forum 2023, 28(1), 4; https://doi.org/10.3390/blsf2023028004 - 6 Nov 2023
Viewed by 1081
Abstract
Thermoplastic starch films (TPS) are an alternative for single-use plastics in packaging. Evaluating large-scale production alternatives that maintain the properties of these bio-based polymers is a crucial factor in understanding their potential industrial use. This preliminary study focuses on testing whether a mathematical [...] Read more.
Thermoplastic starch films (TPS) are an alternative for single-use plastics in packaging. Evaluating large-scale production alternatives that maintain the properties of these bio-based polymers is a crucial factor in understanding their potential industrial use. This preliminary study focuses on testing whether a mathematical model used to predict the drying conditions of ceramic film via tape casting can be adapted to the production of TPS. It also determines the possible drying tape speeds for this type of polymeric film. Full article
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5 pages, 1043 KiB  
Proceeding Paper
Application of Cellulose-Based Film for Broccoli Packaging
by Erika Paulsen, Sofía Barrios and Patricia Lema
Biol. Life Sci. Forum 2023, 28(1), 5; https://doi.org/10.3390/blsf2023028005 - 6 Nov 2023
Viewed by 795
Abstract
Broccoli is a highly perishable vegetable with unique nutritional characteristics. Modified atmosphere packaging (MAP) has proven to be a successful technology to extend broccoli shelf-life. The main disadvantage of MAP is the extensive use of petrochemical-based films resulting in huge quantities of domestic [...] Read more.
Broccoli is a highly perishable vegetable with unique nutritional characteristics. Modified atmosphere packaging (MAP) has proven to be a successful technology to extend broccoli shelf-life. The main disadvantage of MAP is the extensive use of petrochemical-based films resulting in huge quantities of domestic plastic waste. In this study, suitability of a biodegradable cellulose-based film for broccoli florets packaging was evaluated, as an alternative to polypropylene film. Florets packaged in cellulose-based film showed a high mass loss and extremely low in-package O2 concentrations, which made this material unsuitable for broccoli packaging application. Improved gas and water vapor barrier properties should be considered for biodegradable packages, in order to make their application for vegetable packaging feasible. Full article
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5 pages, 1051 KiB  
Proceeding Paper
Starch Nanoparticles Loaded with the Phenolic Compounds from Green Propolis Extract
by Maria Jaízia dos Santos Alves, Wilson Daniel Caicedo Chacon, Alcilene Rodrigues Monteiro and Germán Ayala Valencia
Biol. Life Sci. Forum 2023, 28(1), 6; https://doi.org/10.3390/blsf2023028006 - 6 Nov 2023
Cited by 1 | Viewed by 782
Abstract
Phenolic compounds from propolis extract (PE) have antioxidant and antimicrobial properties; however, extracts from this raw material are not water soluble. This study aimed to stabilize the phenolic compounds from green propolis extract in cassava and potato starch nanoparticles produced by the anti-solvent [...] Read more.
Phenolic compounds from propolis extract (PE) have antioxidant and antimicrobial properties; however, extracts from this raw material are not water soluble. This study aimed to stabilize the phenolic compounds from green propolis extract in cassava and potato starch nanoparticles produced by the anti-solvent precipitation method. The obtained materials displayed a crystalline structure related to starch nanomaterials with a V6h-type crystalline structure. The starch nanoparticles interacted with the phenolic compounds by means of hydrogen bonds and increased the hydrophobicity in the nanomaterials. The developed starch nanomaterials loaded with the phenolic compounds from PE could be potentially used as a novel ingredient in food packaging. Full article
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7 pages, 2265 KiB  
Proceeding Paper
Microcrystals and Microfibers of Cellulose from Acrocomia aculeata (Arecaceae) Characterization
by Shirley Duarte, Magna Monteiro, Porfirio Andrés Campuzano, Natalia Giménez and María Cristina Penayo
Biol. Life Sci. Forum 2023, 28(1), 8; https://doi.org/10.3390/blsf2023028008 - 9 Nov 2023
Viewed by 750
Abstract
In the context of the so-called lignocellulose bio-refinery, the coconut shell (S) and pulp (P) of Acrocomia aculeata (Arecaceae) are interesting agro-industrial wastes that can be used as feedstock for the production of high value-added products. The aim of this work was to [...] Read more.
In the context of the so-called lignocellulose bio-refinery, the coconut shell (S) and pulp (P) of Acrocomia aculeata (Arecaceae) are interesting agro-industrial wastes that can be used as feedstock for the production of high value-added products. The aim of this work was to evaluate these lignocellulosic residues S and P, to obtain the microcrystal (MCC) and microfiber (MFC) of cellulose, and to characterize them to propose possible applications. First, cellulose content in the raw materials was determined, being 39.69% and 45.42% for both (S and P)) respectively, respectively. Then, the purification of residues was carried out via alkaline and bleaching treatments. Next, in order to obtain MCC and MFC from the purified cellulose, a chemical treatment with HCl (for MCC) and a mechanical treatment with a blender (for MFC) were performed. The size and morphology were observed via MEB, and properties were characterized using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and differential thermogravimetric analysis (DTG). Full article
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5 pages, 1614 KiB  
Proceeding Paper
Mechanical Properties of Pineapple Nanocellulose/Epoxy Resin Composites
by Gabriela Álvarez Véliz, Jorge Iván Cifuentes, Diego Batista, Mary Lopretti, Yendry Corrales, Melissa Camacho and José Roberto Vega-Baudrit
Biol. Life Sci. Forum 2023, 28(1), 9; https://doi.org/10.3390/blsf2023028009 - 13 Nov 2023
Cited by 1 | Viewed by 1233
Abstract
A study of materials for wind turbine blades with nanotechnology—from the energy point of view—is an essential topic because resources and fossil fuels are running out. Human beings need to create alternative energies, including wind energy. This research aims to improve the mechanical [...] Read more.
A study of materials for wind turbine blades with nanotechnology—from the energy point of view—is an essential topic because resources and fossil fuels are running out. Human beings need to create alternative energies, including wind energy. This research aims to improve the mechanical properties of epoxy resin wind turbine blades by incorporating nanocelluloses obtained from pineapple residues. To determine the quality of the nanobiocomposites, materials with different epoxy resin–nanocellulose ratios were prepared. The mechanical properties of tension, compression, and bending were evaluated, and hardness tests of the material were conducted. The results indicated a general improvement in all the mechanical properties considered over the material without the nanocellulose. Full article
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12 pages, 2972 KiB  
Proceeding Paper
Biofilms Functionalized Based on Bioactives and Nanoparticles with Fungistatic and Bacteriostatic Properties for Food Packing Uses
by Gabriela Lluberas, Diego Batista-Menezes, Juan Miguel Zuñiga-Umaña, Gabriela Montes de Oca-Vásquez, Nicole Lecot, José Roberto Vega-Baudrit and Mary Lopretti
Biol. Life Sci. Forum 2023, 28(1), 10; https://doi.org/10.3390/blsf2023028010 - 13 Nov 2023
Viewed by 1115
Abstract
The objective of this work was to formulate PVA films with the addition of modified phenols, chitosan, silver, or copper nanoparticles with fungistatic and bacteriostatic activity. The films were characterized by Fourier transform infrared spectroscopy, thermogravimetry analysis, differential scanning calorimetry, and scanning electron [...] Read more.
The objective of this work was to formulate PVA films with the addition of modified phenols, chitosan, silver, or copper nanoparticles with fungistatic and bacteriostatic activity. The films were characterized by Fourier transform infrared spectroscopy, thermogravimetry analysis, differential scanning calorimetry, and scanning electron microscopy. The bacteriostatic activity against Salmonella gallinarum and Lactobacillus acidophilus, and the fungistatic activity against Penicillium acidophilus were determined. The results indicated that the addition of phenols enhanced the effect on the stability of the chemical structure of the PVA film. PVA films with modified bioactives and nanoparticles inhibited the colonization of the microorganisms tested, indicating germicidal control. Full article
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8 pages, 278 KiB  
Proceeding Paper
Food Packaging Film Preparation: From Conventional to Biodegradable and Green Fabrication
by Omayra B. Ferreiro and Magna Monteiro
Biol. Life Sci. Forum 2023, 28(1), 11; https://doi.org/10.3390/blsf2023028011 - 14 Nov 2023
Cited by 1 | Viewed by 1681
Abstract
It is undeniable that suitable packaging will extend the shelf life of the food. The packaging industry has had to renew and innovate in a world where consumers are increasingly environmentally conscious in order to deal with the impact of the production of [...] Read more.
It is undeniable that suitable packaging will extend the shelf life of the food. The packaging industry has had to renew and innovate in a world where consumers are increasingly environmentally conscious in order to deal with the impact of the production of petroleum-derived plastics and the management of the waste generated by them. In this way, the use of biopolymers has been proposed, mainly those produced from renewable sources and with biodegradability and/or compostability properties. However, these types of materials are more expensive and do not have the same performance as petroleum-derived materials. Besides, the technologies for film preparation are not adapted for these materials. Therefore, new technologies must be studied and implemented to make the packaging industry a sustainable industry. Recently, non-solvent phase inversion (NIPS) and electrospinning techniques, which are widely used for membrane fabrication, have been proposed for the fabrication of films for food packaging applications from biopolymers and green solvents. Full article
10 pages, 303 KiB  
Proceeding Paper
Biotransformation of Rice Husk into Phenolic Extracts by Combined Solid Fermentation and Enzymatic Treatment
by Maria Inês Dias, José Pinela, Tânia C. S. P. Pires, Filipa Mandim, Maria-Filomena Barreiro, Lillian Barros, José Roberto Vega-Baudrit, Isabel C. F. R. Ferreira and Mary Lopretti
Biol. Life Sci. Forum 2023, 28(1), 12; https://doi.org/10.3390/blsf2023028012 - 14 Nov 2023
Viewed by 946
Abstract
Biotechnology is essential for developing profitable and productive techniques to obtain metabolites. Two technologies can be used: solid or liquid fermentation and enzymatic treatments. In this context, the objective of this work was to evaluate the use of rice husk, a lignocellulosic material, [...] Read more.
Biotechnology is essential for developing profitable and productive techniques to obtain metabolites. Two technologies can be used: solid or liquid fermentation and enzymatic treatments. In this context, the objective of this work was to evaluate the use of rice husk, a lignocellulosic material, to obtain bioactive compounds by lignin oxidative transformation and demethoxylation, respectively, through enzymatic treatments of P. chrysosporium and G. trabeum. In the first step, solid fermentation was used to obtain the enzyme Lig. Peroxidase and methoxyl hydrolase were quantified as 80 UE and 50 UE, respectively. This enzyme concentrate was lyophilized and used to prepare an enzymatic consortium (240 UE LigP and 150 UE metH) applied in the second phase of enzymatic treatment. The overall process involved 20 days in the solid fermentation step and 2 h for the enzymatic treatment. The obtained products were characterized by having veratryl alcohol and veratryl aldehyde at contents of 70.4 ± 0.1 and 23.3 ± 0.3 mg/g, respectively. Moreover, the analyzed products did not show cytotoxicity but revealed antioxidant and bacteriostatic activities. No anti-inflammatory activity was detected. In the context of circular economy, the obtained results pointed out the use of combined solid fermentation and enzymatic treatment as a viable strategy to valorize rice husk. The applications of these bioactive compounds presenting bactericidal and bacteriostatic activity and not showing toxicity are very common in medicine, agriculture, and environmental health, among others, and can be incorporated both in free systems and immobilized in spheres, capsules or biopolymer films, which is an important input for obtaining functionalized materials that are in high demand today. Full article
1239 KiB  
Proceeding Paper
Lactide Synthesis Using ZnO Aqueous Nanoparticles as Catalysts
by Shirley Duarte, Axel Dullak, Francisco P. Ferreira, Marcelo Oddone and Darío Riveros
Biol. Life Sci. Forum 2023, 28(1), 13; https://doi.org/10.3390/blsf2023028013 - 9 May 2023
Viewed by 754
Abstract
The increasing global consumption of conventional plastics has led to significant environmental challenges due to their resistance to degradation and dependency on petroleum, a volatile resource. In this context, polylactic acid (PLA) has emerged as a promising biopolymer alternative, offering excellent physical and [...] Read more.
The increasing global consumption of conventional plastics has led to significant environmental challenges due to their resistance to degradation and dependency on petroleum, a volatile resource. In this context, polylactic acid (PLA) has emerged as a promising biopolymer alternative, offering excellent physical and mechanical properties while being derived from renewable resources. The synthesis of PLA involves the production of lactide, a crucial cyclic monomer. This study focuses on lactide synthesis using zinc oxide (ZnO) nanoparticles as catalysts. The synthesis process consists of three stages: the dehydration of lactic acid, oligomerization to obtain a PLA oligomer, and depolymerization with simultaneous distillation to produce lactide. The ZnO nanoparticle catalyst proved to be highly efficient in regulating the molecular weight of the oligomer during depolymerization, which directly impacts the molecular weight of the PLA. The lactide purification using ethyl acetate resulted in an average purity of 90.0 ± 1.79%, demonstrating the effectiveness of the purification process. The quantification of lactide through high-performance liquid chromatography (HPLC) showed excellent linearities, allowing for the accurate determination of lactide content. The lactide synthesis yielded 77–80%, and the stability of the synthesized lactide was confirmed through a second purity determination after four months, with only a 1.7% loss in lactide content. Overall, this study showcases the feasibility of lactide synthesis using ZnO nanoparticles as catalysts and contributes valuable insights into producing high-quality lactides for PLA manufacturing. Full article
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