Breast cancer represents one of the leading causes of death for which new strategies of treatment are intensively studied and tested. Single-walled carbon nanotubes (SWCNTs) represent promising tools in the treatment of cancer and can be used as transporters of drugs due to their unique properties [
1]. Furthermore, advanced cellular models, such as multicellular tumor spheroids (MCTSs), are exploited for the study of cancer mechanisms. MCTSs represent 3D cellular structures composed by three layers of cells found in different stages of the cell cycle: the first one is characterized by proliferative cells, followed by cells in a quiescent state, and the last one in the spheroid’s center is represented by necrotic cells [
2]. In this context, we aimed to investigate the cytotoxic effects of SWCNTs loaded with cisplatin (CDDP) in breast cancer MCTSs.
SWCNTs were functionalized with carboxyl groups, resulting in SWCNT-COOH, which was mixed with dimethylformamide and CDDP to obtain the SWCNT-COOH-CDDP nanoconjugates. SWCNT-COOH-CDDP was characterized by inductively coupled plasma mass spectrometry, Fourier-transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDX), and Raman spectroscopy. MCTSs were generated from MDA-MB-231 breast cancer cells in Dulbecco’s Modified Eagle Medium with 2.5% Matrigel, using NunclonTM SpheraTM Microplates. On the second day of culture, MCTSs were treated with doses of 1, 4 µg/mL SWCNTs and 0.6, 2.52 µg/mL CDDP for 24 and 48 h. Untreated MCTSs were used as the control. The MCTSs’ morphology was analyzed using optical microscopy while cellular viability was assessed through a fluorescence method, using LIVE/DEAD assay. Cell death was evaluated by analyzing the expression of pro-apoptotic (Bax) and autophagic (Beclin-1) proteins using the Western blot technique.
Our results indicated that the concentration of CDDP encapsulated in SWCNT-COOH is 192.82 µg/mL. Also, the EDX spectrum highlighted the presence of Pt ions in the SWCNT-COOH-CDDP sample while the FTIR results indicated a covalent bond between CDDP and SWCNT-COOH. The analysis of optical microscopy images indicated a reduction in the size of SWCNT-COOH-CDDP-treated MCTSs and the detachment of cells from the proliferative layer after 48 h, compared with the effects induced by free components, which presented high biocompatibility. Fluorescence images revealed the presence of a necrotic center surrounded by live cells in all tested conditions. However, when MCTSs were treated with 4 µg/mL SWCNT-COOH-CDDP, the detachment of cells from the edge was noticed, in correlation with optical microscopy images. The expression of the Bax protein increased after 24 and 48 h of exposure to 4 µg/mL SWCNT-COOH-CDDP relative to the control but not in the presence of 1 µg/mL SWCNT-COOH-CDDP. In the same time, the expression of Beclin-1 was inhibited after 24 and 48 h of exposure with 1 µg/mL and 4 µg/mL SWCNT-COOH-CDDP, respectively. A slight increase of Beclin-1 expression was observed after the treatment of MCTSs with 4 µg/mL SWCNT-COOH-CDDP for 48 h. No significant changes were observed in the presence of 1 µg/mL SWCNT-COOH-CDDP after 48 h.
Considering the results of the present study, we conclude that SWCNT-COOH-CDDP nanoconjugates have the potential to induce significant morphological changes in MCTSs and to initiate apoptosis pathway through the activation of the Bax protein and the inhibition of the autophagy process.
