Synthesis and Characterization of a New Cr(III) Complex with 5-Hydroxyflavone as a Potential Antidiabetic Agent †
by
, ,
Valeria-Nicoleta Vătavu
1, 
Mihaela Badea
2, 
Rodica Olar
2,
Ariana Hudiță
3,
Bianca Gălățeanu
3,
Alexandra Cristina Munteanu
1 and
Valentina Uivarosi
1,*
1
Department of General and Inorganic Chemistry, Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, 6 Traian Vuia Str, 020956 Bucharest, Romania
2
Department of Inorganic Chemistry, Faculty of Chemistry, University of Bucharest, 90-92 Panduri Str., 050663 Bucharest, Romania
3
Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, Romania
*
Author to whom correspondence should be addressed.
†
Presented at the 15th International Symposium “Priorities of Chemistry for a Sustainable Development” PRIOCHEM, Bucharest, Romania, 30th October–1st November 2019.
Proceedings 2019, 29(1), 118; https://doi.org/10.3390/proceedings2019029118
Published: 18 October 2019
(This article belongs to the Proceedings of Priorities of Chemistry for a Sustainable Development-PRIOCHEM)
Diabetes mellitus (DM) is a disease characterized by chronic hyperglycemia caused by absolute or relative insulin deficiency and/or insulin resistance, which in turn affects glucose, protein, and lipid metabolism, and ultimately causes characteristic secondary complications [1]. The adipose tissue provides a link between diabetes and obesity [2]. The accumulation of adipose tissue, as in obesity, and its deficiency, as in acquired or inherited lipodystrophy, are both associated with insulin resistance [3]. The aim of this study was to synthesize a new compound as a potential antidiabetic agent. In this regard, the antiadipogenic activity of the complex of Cr(III) with 5-hydroxyflavone was evaluated in vitro on human adipose tissue-derived stem cells (hASCs) engaged in adipogenesis.
The Cr(III) complex was synthesized by refluxing an ethanolic solution containing 5-hydroxyflavone and CrCl3 in a molar ratio metal to ligand of 1:3. The red solid product obtained was characterized by elemental and thermal analyses and several spectroscopic techniques (Electrospray Ionization-Mass Spectrometry, Infrared, Ultraviolet-Visible, fluorescence spectroscopy). The antiadipogenic potential of the chromium (III) complex with 5-hydroxyflavone on hASCs was evaluated over 3 weeks after its administration in an adipogenic environment. The expression of perilipin was evaluated both quantitatively (flow cytometry) and qualitatively (fluorescence microscopy).
The physicochemical analyses revealed the formation of mononuclear complexes with 1:3 metal to ligand stoichiometry. Histograms obtained by flow cytometry showed that the expression of perilipin decreases significantly in the presence of the chromium (III) complex with 5-hydroxyflavone.
The newly synthesized complex of Cr(III) with 5-hydroxyflavone displayed in vitro antiadipogenic activity, a premise for further in vivo studies using a murine model of diabetes.
Acknowledgments
This research was financially supported by “Carol Davila” University of Medicine and Pharmacy through Contract no. 23PFE/17.10.2018 funded by the Ministry of Research and Innovation within PNCDI III, Program 1 (Development of the National RD system), Subprogram 1.2 (Institutional Performance) RDI excellence funding projects.
References
- Diabetes Mellitus: Reports of a WHO Study Group; WHO Technical Report Series; WHO: Geneva, Switzerland, 1985; Volume 727, p. 10.
- Grundy, S.M. Adipose tissue and metabolic syndrome: too much, too little or neither. Eur. J. Clin. Investig. 2015, 45, 1209–1217. [Google Scholar] [CrossRef] [PubMed]
- Scheen, A.J. Pathophysiology of type 2 diabetes. Acta Clin. Belg. 2003, 58, 335–341. [Google Scholar] [CrossRef] [PubMed]
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MDPI and ACS Style
Vătavu, V.-N.; Badea, M.; Olar, R.; Hudiță, A.; Gălățeanu, B.; Munteanu, A.C.; Uivarosi, V. Synthesis and Characterization of a New Cr(III) Complex with 5-Hydroxyflavone as a Potential Antidiabetic Agent. Proceedings 2019, 29, 118. https://doi.org/10.3390/proceedings2019029118
AMA Style
Vătavu V-N, Badea M, Olar R, Hudiță A, Gălățeanu B, Munteanu AC, Uivarosi V. Synthesis and Characterization of a New Cr(III) Complex with 5-Hydroxyflavone as a Potential Antidiabetic Agent. Proceedings. 2019; 29(1):118. https://doi.org/10.3390/proceedings2019029118
Chicago/Turabian StyleVătavu, Valeria-Nicoleta, Mihaela Badea, Rodica Olar, Ariana Hudiță, Bianca Gălățeanu, Alexandra Cristina Munteanu, and Valentina Uivarosi. 2019. "Synthesis and Characterization of a New Cr(III) Complex with 5-Hydroxyflavone as a Potential Antidiabetic Agent" Proceedings 29, no. 1: 118. https://doi.org/10.3390/proceedings2019029118
