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  • Open Access

18 September 2025

Maslinic Acid Nanoemulsion: In Vitro and In Ovo Assessment †

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1
Faculty of Pharmacy, “Victor Babeș” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timișoara, Romania
2
Research and Processing Center for Medicinal and Aromatic Plants (Plant-Med), “Victor Babeș” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timișoara, Romania
3
Research Center for Pharmaco-Toxicological Evaluations (FARMTOX), “Victor Babeș” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timișoara, Romania
4
Formulation and Technology of Drugs Research Center (FORMTEHMED), “Victor Babeș” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timișoara, Romania
This article belongs to the Proceedings International Conference on Interdisciplinary Approaches and Emerging Trends in Pharmaceutical Doctoral Research: Innovation and Integration
Nowadays, natural products are highly investigated in cancer research due to their wide distribution, numerous beneficial effects, and minimal side effects. Maslinic acid (MA) is found in different plant sources, but is mainly obtained from the olive tree []. This pentacyclic triterpene has an anti-tumor effect in various types of cancer, namely, colon cancer, gastric cancer, lung cancer, renal carcinoma, ovarian cancer, breast cancer, myeloma, pancreatic cancer, skin cancer, and leukemia, among others. It has reduced bioavailability due to its low solubility in aqueous solutions []; thus, to improve it, various formulations can be prepared. The purpose of the present study was to investigate the in vitro and in ovo effects of a maslinic acid nanoemulsion (MA-NE). The MA-NE was tested on two melanoma cell lines, A375 and SK-MEL-28, and on the chorioallantoic membrane, CAM. The sample produced a dose-dependent decrease in the human melanoma cell lines. In the tumor angiogenesis model, the formulation decreased cell migration. The results obtained indicate that MA-NE has an anti-melanoma effect and reduces tumor development within the tumor angiogenesis model. Further studies will be performed to elucidate the underlying mechanism of action.

Author Contributions

Conceptualization, I.Z.M.-P. and Ș.A.; methodology, I.Z.M.-P., E.-A.M., L.B., D.M., L.V. and Ș.A.; validation, I.Z.M.-P., Ș.A., C.A.D. and C.D.; investigation, I.Z.M.-P., D.U., E.-A.M., L.B., D.M., L.V. and Ș.A.; data curation, D.U., E.-A.M. and L.B.; writing—original draft preparation, I.Z.M.-P.; writing—review and editing, Ș.A. and C.D.; supervision, C.D. and C.A.D.; project administration, I.Z.M.-P.; funding acquisition, I.Z.M.-P. and C.A.D. All authors have read and agreed to the published version of the manuscript.

Funding

This work was supported by a grant of the Romanian Ministry of Education and Research, CNCS-UEFISCDI, Project number PN-III-P1-1.1-PD-2019-1231, within PNCDI III, PD 206/2020. Project Director Ioana Zinuca Magyari-Pavel.

Institutional Review Board Statement

Not applicable.

Data Availability Statement

Data are contained within the abstract.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Ooi, K.X.; Poo, C.L.; Subramaniam, M.; Cordell, G.A.; Lim, Y.M. Maslinic acid exerts anticancer effects by targeting cancer hallmarks. Phytomedicine 2023, 110, 154631. [Google Scholar] [CrossRef] [PubMed]
  2. Aguilera-Garrido, A.; Graván, P.; Navarro-Marchal, S.A.; Medina-O’Donnell, M.; Parra, A.; Gálvez-Ruiz, M.J.; Marchal, J.A.; Galisteo-González, F. Maslinic acid solid lipid nanoparticles as hydrophobic anticancer drug carriers: Formulation, in vitro activity and in vivo biodistribution. Biomed. Pharmacother. 2023, 163, 114828. [Google Scholar] [CrossRef] [PubMed]
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