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Abstract

Optimizing Amino Acid Derivatization in Food Matrices †

by
Francisca Santos
*,
Valentina Domingues
,
Cristina Soares
and
Cristina Delerue-Matos
REQUIMTE/LAQV, Instituto Superior de Engenharia do Porto, Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida, 431, 4249-015 Porto, Portugal
*
Author to whom correspondence should be addressed.
Presented at the 3rd International Electronic Conference on Processes—Green and Sustainable Process Engineering and Process Systems Engineering (ECP 2024), 29–31 May 2024; Available online: https://sciforum.net/event/ECP2024.
Proceedings 2024, 105(1), 104; https://doi.org/10.3390/proceedings2024105104
Published: 28 May 2024
Amino acids are the fundamental units that proteins are composed of. They are organic molecules essential for life and play vital roles in our bodies. Some amino acids are produced by our bodies (non-essential amino acids), while others need to be obtained through food (essential amino acids). In addition, these molecules perform crucial functions, such as speeding up biochemical reactions (enzymes), acting as chemical signals (hormones), forming structural components (muscles, tendons, and nails), and conferring diversity to each specific protein [1]. Methods for the derivatization and analysis of amino acids often involve chemical modification to improve detection sensitivity, with techniques such as high-performance liquid chromatography (HPLC) being typical. However, confirm acid derivatization reaction in food matrices through high-performance liquid chromatography (HPLC) analysis to improve the sensitivity and precision of amino acid quantification. To optimize the amino acid derivatization reaction, a systematic approach was used, varying the concentrations of OPA and 3-MPA (10, 15, 20, 25, and 30 mM), as well as the concentration of FMOC-Cl (1, 2, 5, and 10 mM) and the reaction time (0.5, 1, 1.5 and 2 min). In the HPLC system, an acetate buffer was used as the mobile phase, and a solution of acetonitrile, methanol, and water was used. With this experimental method, it was possible to identify the ideal conditions using a specific combination of concentrations of OPA, 3-MPA, and FMOC-Cl, together with the time of occurrence, which was investigated for greater sensitivity and precision in the quantification of amino acids. This study improved the methodology for analyzing amino acids in food matrices using HPLC, enabling more reliable and accurate results.

Author Contributions

Conceptualization, all authors; methodology, F.S. and C.S.; formal analysis, F.S. and C.S.; investigation, F.S. and C.S.; resources, V.D. and C.D.-M.; writing—original draft preparation, F.S. and C.S.; writing—review and editing, all authors; supervision, C.S. and V.D.; project administration, C.D.-M.; funding acquisition, V.D. and C.D.-M. All authors have read and agreed to the published version of the manuscript.

Funding

This work received support and help from FCT/MCTES (LA/P/0008/2020 DOI:10.54499/LA/P/0008/2020, UIDP/50006/2020 DOI:10.54499/UIDP/50006/2020 and UIDB/50006/2020 DOI:10.54499/UIDB/50006/2020).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Conflicts of Interest

The authors declare no conflict of interest.

Reference

  1. Ling, Z.-N.; Jiang, Y.-F.; Ru, J.-N.; Lu, J.-H.; Ding, B.; Wu, J. Amino Acid Metabolism in Health and Disease. Signal Transduct. Target. Ther. 2023, 8, 345. [Google Scholar] [CrossRef]
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Share and Cite

MDPI and ACS Style

Santos, F.; Domingues, V.; Soares, C.; Delerue-Matos, C. Optimizing Amino Acid Derivatization in Food Matrices. Proceedings 2024, 105, 104. https://doi.org/10.3390/proceedings2024105104

AMA Style

Santos F, Domingues V, Soares C, Delerue-Matos C. Optimizing Amino Acid Derivatization in Food Matrices. Proceedings. 2024; 105(1):104. https://doi.org/10.3390/proceedings2024105104

Chicago/Turabian Style

Santos, Francisca, Valentina Domingues, Cristina Soares, and Cristina Delerue-Matos. 2024. "Optimizing Amino Acid Derivatization in Food Matrices" Proceedings 105, no. 1: 104. https://doi.org/10.3390/proceedings2024105104

APA Style

Santos, F., Domingues, V., Soares, C., & Delerue-Matos, C. (2024). Optimizing Amino Acid Derivatization in Food Matrices. Proceedings, 105(1), 104. https://doi.org/10.3390/proceedings2024105104

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