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Review

The Advantages of EPR Spectroscopy in Exploring Diamagnetic Metal Ion Binding and Transfer Mechanisms in Biological Systems

Department of Chemistry and the Institute of Nanotechnology and Advanced Materials (BINA), Bar-Ilan University, Ramat-Gan 5290002, Israel
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Author to whom correspondence should be addressed.
Academic Editors: Angeliki Giannoulis and Dinar Abdullin
Magnetochemistry 2022, 8(1), 3; https://doi.org/10.3390/magnetochemistry8010003
Received: 21 November 2021 / Revised: 19 December 2021 / Accepted: 22 December 2021 / Published: 26 December 2021
(This article belongs to the Special Issue EPR Spectroscopy in Chemistry and Biology)
Electron paramagnetic resonance (EPR) spectroscopy has emerged as an ideal biophysical tool to study complex biological processes. EPR spectroscopy can follow minor conformational changes in various proteins as a function of ligand or protein binding or interactions with high resolution and sensitivity. Resolving cellular mechanisms, involving small ligand binding or metal ion transfer, is not trivial and cannot be studied using conventional biophysical tools. In recent years, our group has been using EPR spectroscopy to study the mechanism underlying copper ion transfer in eukaryotic and prokaryotic systems. This mini-review focuses on our achievements following copper metal coordination in the diamagnetic oxidation state, Cu(I), between biomolecules. We discuss the conformational changes induced in proteins upon Cu(I) binding, as well as the conformational changes induced in two proteins involved in Cu(I) transfer. We also consider how EPR spectroscopy, together with other biophysical and computational tools, can identify the Cu(I)-binding sites. This work describes the advantages of EPR spectroscopy for studying biological processes that involve small ligand binding and transfer between intracellular proteins. View Full-Text
Keywords: copper cycle; copper metabolism; CW-EPR; DEER; EPR spectroscopy copper cycle; copper metabolism; CW-EPR; DEER; EPR spectroscopy
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MDPI and ACS Style

Meron, S.; Shenberger, Y.; Ruthstein, S. The Advantages of EPR Spectroscopy in Exploring Diamagnetic Metal Ion Binding and Transfer Mechanisms in Biological Systems. Magnetochemistry 2022, 8, 3. https://doi.org/10.3390/magnetochemistry8010003

AMA Style

Meron S, Shenberger Y, Ruthstein S. The Advantages of EPR Spectroscopy in Exploring Diamagnetic Metal Ion Binding and Transfer Mechanisms in Biological Systems. Magnetochemistry. 2022; 8(1):3. https://doi.org/10.3390/magnetochemistry8010003

Chicago/Turabian Style

Meron, Shelly, Yulia Shenberger, and Sharon Ruthstein. 2022. "The Advantages of EPR Spectroscopy in Exploring Diamagnetic Metal Ion Binding and Transfer Mechanisms in Biological Systems" Magnetochemistry 8, no. 1: 3. https://doi.org/10.3390/magnetochemistry8010003

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