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Fermentation 2018, 4(2), 24; https://doi.org/10.3390/fermentation4020024

Effect of Sludge Concentration and Crude Glycerol Matrix as a Substrate on the Production of Single-Cell Oil by Oleaginous Yeast Yarrowia lipolytica SKY7

INRS ETE, 490 rue de la Couronne, Quebec City, QC G1K 9A9, Canada
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Received: 28 February 2018 / Revised: 24 March 2018 / Accepted: 29 March 2018 / Published: 2 April 2018
(This article belongs to the Special Issue Microbial Production of Added-value Products from Renewable Resources)
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Abstract

The disposal of excess crude glycerol produced by the booming biodiesel industry and wastewater sludge solid waste has become a severe problem, and alternate routes of use and valorization of these waste byproducts are needed. The use of cheaply available wastewater sludge solids in fermentation media is very much desirable to reduce the cost of production. The strains of Yarrowia lipolytica can assimilate a wide array of waste substrates, such as crude glycerol, waste cooking oil, starch wastewater, and cellulosic. This study optimized the concentration of wastewater sludge solids (5–35 g/L) to be used with crude glycerol in fermentation media to produce microbial oil as feedstock for biodiesel production. The results indicated that 20 g/L of sludge solids with 40 g/L of crude glycerol resulted in highest lipid content of 29.35% in 96 h. Further, assuming wet extraction of lipids, it was found that at least 11.2% or higher lipid content is required for this process to have an overall positive net solid waste reduction. Insignificant inhibition was observed by the crude glycerol used in this study as compared to pure glycerol, which proves it to be an adequate source of carbon substrate for lipid production. View Full-Text
Keywords: crude glycerol; Yarrowia lipolytica; lipid; biodiesel; municipal wastewater sludge; single cell oil crude glycerol; Yarrowia lipolytica; lipid; biodiesel; municipal wastewater sludge; single cell oil
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Ram, S.K.; Tyagi, R.D.; Drogui, P. Effect of Sludge Concentration and Crude Glycerol Matrix as a Substrate on the Production of Single-Cell Oil by Oleaginous Yeast Yarrowia lipolytica SKY7. Fermentation 2018, 4, 24.

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