External Quality Assessment Evaluating the Ability of Dutch Clinical Microbiological Laboratories to Identify Candida auris

Buil, Jochem B.; van der Lee, Henrich A. L.; Curfs-Breuker, Ilse; Verweij, Paul E.; Meis, Jacques F.

doi:10.3390/jof5040094

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External Quality Assessment Evaluating the Ability of Dutch Clinical Microbiological Laboratories to Identify Candida auris

Jochem B. Buil

^1,2,*

Henrich A. L. van der Lee

^1,2,

Ilse Curfs-Breuker

^1,2,3,

Paul E. Verweij

^1,2

and

Jacques F. Meis

^1,2,3

Department of Medical Microbiology, Radboud University Medical Center, 6525GA Nijmegen, The Netherlands

Center of Expertise in Mycology Radboudumc/CWZ, 6525GA Nijmegen, The Netherlands

Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital (CWZ), 6532SZ Nijmegen, The Netherlands

Author to whom correspondence should be addressed.

J. Fungi 2019, 5(4), 94; https://doi.org/10.3390/jof5040094

Received: 30 August 2019 / Revised: 30 September 2019 / Accepted: 30 September 2019 / Published: 7 October 2019

(This article belongs to the Special Issue Candida auris)

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Abstract

Background: Candida auris is a yeast that is causing nosocomial outbreaks in healthcare facilities around the world. There is a risk of the misidentification of C. auris with matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)—when libraries are used that lack C. auris spectra, or when conventional biochemical methods are used. Methods: We conducted an external quality assessment to evaluate the ability of Dutch clinical microbiological laboratories to identify C. auris, and to raise awareness about the risk of misidentification. Results: 35/47 participating laboratories were able to identify C. auris correctly. Only 2/14 labs that potentially misidentified C. auris with their primary identification methods specified that they would perform additional tests to exclude C. auris when appropriate. 45/47 labs used MALDI-TOF MS systems to identify Candida species. Conclusions: There was a lack of awareness about the potential misidentification of C. auris in many labs that used MALDI-TOF MS with libraries that lacked C. auris spectra, and labs that used Vitek 2. However, as the currently available MALDI-TOF MS libraries in The Netherlands contain several C. auris spectra, we expect that currently almost all participating laboratories are able to identify C. auris correctly, as 45/47 participating laboratories use MALDI-TOF MS as their primary yeast identification method. View Full-Text

Keywords: C. auris; MALDI-TOF MS; misidentification

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MDPI and ACS Style

Buil, J.B.; van der Lee, H.A.L.; Curfs-Breuker, I.; Verweij, P.E.; Meis, J.F. External Quality Assessment Evaluating the Ability of Dutch Clinical Microbiological Laboratories to Identify Candida auris. J. Fungi 2019, 5, 94. https://doi.org/10.3390/jof5040094

AMA Style

Buil JB, van der Lee HAL, Curfs-Breuker I, Verweij PE, Meis JF. External Quality Assessment Evaluating the Ability of Dutch Clinical Microbiological Laboratories to Identify Candida auris. Journal of Fungi. 2019; 5(4):94. https://doi.org/10.3390/jof5040094

Chicago/Turabian Style

Buil, Jochem B., Henrich A.L. van der Lee, Ilse Curfs-Breuker, Paul E. Verweij, and Jacques F. Meis. 2019. "External Quality Assessment Evaluating the Ability of Dutch Clinical Microbiological Laboratories to Identify Candida auris" Journal of Fungi 5, no. 4: 94. https://doi.org/10.3390/jof5040094

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