Identification and Analysis of InDel Variants in Key Hippo Pathway Genes and Their Association with Growth Traits in Four Chinese Sheep Breeds
Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Ethics Statement
2.2. Animal Samples and Data Collection
2.3. Genomic DNA Isolation and DNA Pool Construction
2.4. Primer Design, PCR Amplification, and Indel Genotyping
2.5. Estimation of Population Genetic Parameters and Statistical Analysis
3. Results
3.1. Identification and Analysis of InDel Loci of MST1/2 Gene
3.1.1. Identification of InDel Variants in the MST1/2 Gene
3.1.2. Genetic Parameter Analysis of InDel Loci of MST1/2 Gene in Sheep
3.1.3. Linkage Disequilibrium Analysis for InDel Loci in the MST1/2 Gene
3.1.4. Association Between InDel Loci and Growth Traits in Sheep MST1/2 Gene
3.2. Identification and Analysis of InDel Loci of YAP/TAZ Gene
3.2.1. Identification of InDel Variants in the YAP/TAZ Gene
3.2.2. Genetic Parameter Analysis of InDel Loci of YAP/TAZ Gene in Sheep
3.2.3. Linkage Disequilibrium Analysis for InDel Loci in the YAP and TAZ Genes
3.2.4. Association Between InDel Loci and Growth Traits in Sheep YAP/TAZ Gene
3.3. Identification and Analysis of InDel Loci of the MOB1A/B Gene
3.3.1. Identification of InDel Variants in the MOB1A/B Gene
3.3.2. Genetic Parameter Analysis of InDel Loci of the MOB1A Gene
3.3.3. Genetic Parameter Analysis of InDel Loci of MOB1A Gene
3.3.4. Association Between InDel Loci in the MOB1A Gene and Growth Traits in Sheep
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
MDPI | Multidisciplinary Digital Publishing Institute |
GPCRs | G Protein-Coupled Receptors |
He | Expected heterozygosity |
Ho | Observed heterozygosity |
HWE | Hardy–Weinberg equilibrium |
InDel | Insertion/Deletion |
LATS1/2 | Large tumor suppressor 1/2 |
MAS | Marker-assisted selection |
MOB1A/B | MOB kinase activator 1A/B |
MST1/2 | Mammalian Sterile 20-like kinases 1/2 |
Ne | Effective number of alleles |
PIC | Polymorphism information content |
SAV1 | Salvador 1 |
TAZ | Transcriptional coactivator with PDZ-binding motif |
YAP | Yes- associated protein |
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Gene | Locus | Primer Sequence | Product Size | InDel Size |
---|---|---|---|---|
MST1 | MST1-S2 | F:CATGCCTCTAAAGATGAG | 102 bp | 10 bp |
R:CAGACTCCGGGAGGACAA | ||||
MST1-S8 | F:GGGCTTTGGTTCTCAGGC | 178 bp | 7 bp | |
R:GTGGGACTACTTCACCATCTGT | ||||
MST1-S17 | F:AAGAGCAAGCCTTCAGCG | 153 bp | 8 bp | |
R:CAGCAGGTTCTCACTCGTTA | ||||
MST2 | MST2-S1 | F:CTACATGCAATCTCAAGT | 114 bp | 12 bp |
R:TAAGAAGTTCAAGGTAAT | ||||
MST2-S4 | F:TTTTCAGTAGTAAGGCTGTT | 181 bp | 17 bp | |
R:CGACCCGTAGTAGGATTT | ||||
MST2-S15 | F:GCCACTTCCAGCTTTGGG | 132 bp | 7 bp | |
R:ACAGACAGAAGAGCGAAA | ||||
MST2-S27 | F:TATGAACTGCCTGGTGAA | 172 bp | 9 bp | |
R:AGGGTGGAAACTATAACC | ||||
MST2-S36 | F:TTCTTCCGAAGGGCACAA | 95 bp | 10 bp | |
R:TCATTTTACACCATCAGC | ||||
MST2-S38 | F:GTTATGGAACTACTACTATTAC | 100 bp | 12 bp | |
R:GGGGTTTGATCTCAATCT | ||||
MST2-S40 | F:AGTTGGTTGTTTCCTGTT | 118 bp | 10 bp | |
R:TTCAGTTAGCCTCAATAC | ||||
YAP | YAP-S4 | F:CTGGCATAGCTGTTTTAA | 133 bp | 11 bp |
R:TAGACATTTGTTCGGGAT | ||||
YAP-S5 | F:AACTTTAGCTTTTGTCACTC | 124 bp | 13 bp | |
R:AAGGGCAGATTCTAACATTA | ||||
YAP-S7 | F:GATCAAACTATGGATTGT | 96 bp | 6 bp | |
R:AGATGTCTGAAAAGGTAA | ||||
YAP-S11 | F:GAAACCCAACTGAAGAAAGG | 118 bp | 10 bp | |
R:ATTTGCACAAAGAAATACCC | ||||
YAP-S15 | F:AAGCTATTGTCATCTACAAC | 104 bp | 11 bp | |
R:CAGTAAACAATATCTAGGGA | ||||
YAP-S17 | F:TACAAAGCAACTAACAACG | 104 bp | 10 bp | |
R:TAGTGCAACTGCTGAAAA | ||||
YAP-S19 | F:CTCCCTTTTCATAATCCACT | 146 bp | 11 bp | |
R:CAGAATCTTGGCAAAGTTGA | ||||
YAP-S21 | F:AGACTAAAGCCCCACCTCCC | 163 bp | 27 bp | |
R:GTGGCATCTAAGTTCATTCA | ||||
TAZ | TAZ-S17 | F:GACACTACAGCTCCAGCAT | 126 bp | 7 bp |
R:TCAGGGACCAGATTCACA | ||||
TAZ-S24 | F:TGGAGGTAGGAGGTGAGA | 164 bp | 6 bp | |
R:CAAGGCTGGTCTAGGATG | ||||
MOB1A | MOB1A-S4 | F:CTGTAAACCAGGAATAGG | 113 bp | 8 bp |
R:CTGGAGGTCTCATAAGCA | ||||
MOB1A-S7 | F:ACAATGGCAACTGAGCTTTC | 145 bp | 11 bp | |
R:ATGGGGTATCTTTTGGGAGG | ||||
MOB1A-S10 | F:TTTTCCCCAATACGTCCC | 120 bp | 17 bp | |
R:CAGATTAGGCAAAGTCAT |
Locus | Breed | Number | Genotypic Frequency | Allelic Frequency | Polymorphism Information | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
n | II | ID | DD | I | D | Ho | He | Ne | PIC | |||
MST1-S2 | LLTS | 61 | 0.541 | 0.344 | 0.115 | 0.713 | 0.287 | 0.344 | 0.409 | 1.693 | 0.326 | p > 0.05 |
STHS | 190 | 0.495 | 0.416 | 0.089 | 0.703 | 0.297 | 0.416 | 0.418 | 1.717 | 0.330 | p > 0.05 | |
TS | 166 | 0.060 | 0.422 | 0.518 | 0.271 | 0.729 | 0.422 | 0.395 | 1.653 | 0.317 | p > 0.05 | |
HS | 201 | 0.478 | 0.388 | 0.134 | 0.672 | 0.328 | 0.388 | 0.441 | 1.788 | 0.344 | p > 0.05 | |
MST1-S8 | TS | 166 | 0.530 | 0.386 | 0.084 | 0.723 | 0.277 | 0.386 | 0.401 | 1.668 | 0.320 | p > 0.05 |
HS | 201 | 0.672 | 0.274 | 0.055 | 0.808 | 0.192 | 0.274 | 0.310 | 1.450 | 0.262 | p > 0.05 | |
MST1-S17 | LLTS | 61 | 0.016 | 0.344 | 0.639 | 0.189 | 0.811 | 0.344 | 0.307 | 1.442 | 0.260 | p > 0.05 |
STHS | 190 | 0.042 | 0.295 | 0.663 | 0.189 | 0.811 | 0.295 | 0.307 | 1.442 | 0.260 | p > 0.05 | |
TS | 166 | 0.006 | 0.187 | 0.807 | 0.099 | 0.901 | 0.187 | 0.178 | 1.217 | 0.162 | p > 0.05 | |
HS | 201 | 0.040 | 0.279 | 0.682 | 0.179 | 0.821 | 0.279 | 0.294 | 1.416 | 0.251 | p > 0.05 |
Locus | Breed | Number | Genotypic Frequency | Allelic Frequency | Polymorphism Information | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
n | II | ID | DD | I | D | Ho | He | Ne | PIC | |||
MST2-S1 | LLTS | 61 | 0.098 | 0.377 | 0.525 | 0.287 | 0.713 | 0.377 | 0.409 | 1.693 | 0.326 | p > 0.05 |
STHS | 190 | 0.079 | 0.368 | 0.553 | 0.263 | 0.737 | 0.368 | 0.388 | 1.633 | 0.313 | p > 0.05 | |
TS | 166 | 0.042 | 0.331 | 0.627 | 0.208 | 0.792 | 0.331 | 0.329 | 1.491 | 0.275 | p > 0.05 | |
HS | 201 | 0.139 | 0.458 | 0.403 | 0.368 | 0.632 | 0.458 | 0.465 | 1.870 | 0.357 | p > 0.05 | |
MST2-S4 | LLTS | 61 | 0.230 | 0.541 | 0.230 | 0.500 | 0.500 | 0.541 | 0.500 | 2.000 | 0.375 | p > 0.05 |
STHS | 190 | 0.311 | 0.474 | 0.216 | 0.547 | 0.453 | 0.474 | 0.496 | 1.982 | 0.373 | p > 0.05 | |
TS | 166 | 0.211 | 0.458 | 0.331 | 0.440 | 0.560 | 0.458 | 0.493 | 1.972 | 0.371 | p > 0.05 | |
HS | 201 | 0.294 | 0.303 | 0.403 | 0.445 | 0.555 | 0.303 | 0.494 | 1.976 | 0.372 | p > 0.05 | |
MST2-S15 | LLTS | 61 | 0.115 | 0.459 | 0.426 | 0.344 | 0.656 | 0.459 | 0.451 | 1.823 | 0.349 | p > 0.05 |
MST2-S27 | LLTS | 61 | 0.000 | 0.230 | 0.770 | 0.115 | 0.885 | 0.230 | 0.204 | 1.256 | 0.183 | p > 0.05 |
STHS | 190 | 0.016 | 0.142 | 0.842 | 0.087 | 0.913 | 0.142 | 0.159 | 1.189 | 0.146 | p > 0.05 | |
TS | 166 | 0.000 | 0.289 | 0.711 | 0.145 | 0.855 | 0.289 | 0.248 | 1.330 | 0.217 | p > 0.05 | |
HS | 201 | 0.005 | 0.214 | 0.781 | 0.112 | 0.888 | 0.214 | 0.199 | 1.248 | 0.179 | p > 0.05 | |
MST2-S36 | STHS | 190 | 0.721 | 0.242 | 0.037 | 0.842 | 0.158 | 0.242 | 0.266 | 1.363 | 0.231 | p > 0.05 |
TS | 166 | 0.500 | 0.410 | 0.090 | 0.705 | 0.295 | 0.410 | 0.416 | 1.712 | 0.329 | p > 0.05 | |
HS | 201 | 0.547 | 0.378 | 0.075 | 0.736 | 0.264 | 0.378 | 0.389 | 1.636 | 0.313 | p > 0.05 | |
MST2-S38 | LLTS | 61 | 0.770 | 0.180 | 0.049 | 0.861 | 0.139 | 0.180 | 0.239 | 1.315 | 0.211 | p > 0.05 |
STHS | 190 | 0.626 | 0.305 | 0.068 | 0.779 | 0.221 | 0.305 | 0.344 | 1.525 | 0.285 | p > 0.05 | |
TS | 166 | 0.494 | 0.373 | 0.133 | 0.681 | 0.319 | 0.373 | 0.434 | 1.768 | 0.340 | p > 0.05 | |
HS | 201 | 0.537 | 0.254 | 0.209 | 0.664 | 0.336 | 0.254 | 0.446 | 1.806 | 0.347 | p > 0.05 | |
MST2-S40 | STHS | 190 | 0.621 | 0.326 | 0.053 | 0.784 | 0.216 | 0.326 | 0.339 | 1.512 | 0.281 | p > 0.05 |
TS | 166 | 0.452 | 0.446 | 0.102 | 0.675 | 0.325 | 0.446 | 0.439 | 1.782 | 0.342 | p > 0.05 | |
HS | 201 | 0.488 | 0.418 | 0.095 | 0.697 | 0.303 | 0.418 | 0.422 | 1.731 | 0.333 | p > 0.05 |
Locus | Breed | Growth Trait | Observed Genotypes (MEANS ± SEM) | p | ||
---|---|---|---|---|---|---|
II | ID | DD | ||||
MST1-S2 | HS | BoH (cm) | 62.53 ± 0.39 a (n = 96) | 61.04 ± 0.38 b (n = 78) | 61.50 ± 0.73 ab (n = 27) | 0.027 |
HS | BOL (cm) | 71.35 ± 0.39 a (n = 96) | 70.90 ± 0.46 a (n = 78) | 69.04 ± 0.58 b (n = 27) | 0.022 | |
MST1-S8 | TS | MFoW (cm) | 12.91 ± 0.12 a (n = 88) | 12.85 ± 0.14 a (n = 64) | 12.07 ± 0.13 b (n = 14) | 0.026 |
MST1-S17 | TS | BoL (cm) | - | 67.71 ± 1.66 b (n = 31) | 70.30 ± 0.45 a (n = 134) | 0.045 |
TS | HeD (cm) | - | 14.25 ± 0.25 b (n = 31) | 14.76 ± 0.09 a (n = 134) | 0.043 | |
HS | CaC(cm) | 6.25 ± 0.27 b (n = 8) | 7.13 ± 0.08 a (n = 56) | 7.15 ± 0.04 a (n = 137) | 0.000 | |
HS | BW (kg) | 28.35 ± 1.26 b (n = 8) | 32.72 ± 0.58 a (n = 56) | 32.38 ± 0.39 a (n = 137) | 0.037 |
Locus | Breed | Growth Trait | Observed Genotypes (MEANS ± SEM) | p | ||
---|---|---|---|---|---|---|
II | ID | DD | ||||
MST2-S1 | HS | BW (kg) | 34.34 ± 0.88 a (n = 28) | 32.33 ± 0.46 b (n = 92) | 31.59 ± 0.50 b (n = 81) | 0.021 |
STHS | Hih (cm) | 63.45 ± 1.24 a (n = 15) | 61.82 ± 0.50 b (n = 70) | 63.53 ± 0.40 a (n = 105) | 0.029 | |
MST2-S4 | LLTS | BoL (cm) | 68.58 ± 2.18 b (n = 14) | 75.77 ± 1.35 a (n = 33) | 79.83 ± 3.65 a (n = 14) | 0.026 |
LLTS | BoH (cm) | 70.00 ± 1.86 b (n = 14) | 77.10 ± 1.28 a (n = 33) | 80.17 ± 2.73 a (n = 14) | 0.021 | |
LLTS | Hih (cm) | 74.50 ± 1.61 b (n = 14) | 77.83 ± 1.43 b (n = 33) | 98.00 ± 17.07 a (n = 14) | 0.031 | |
STHS | CaC (cm) | 7.16 ± 0.10 a (n = 59) | 7.21 ± 0.07 a (n = 90) | 6.79 ± 0.12 b (n = 41) | 0.008 | |
MST2-S15 | LLTS | BW(kg) | 32.50 ± 1.72 b (n = 7) | 60.71 ± 3.21 a (n = 28) | 52.53 ± 4.09 a (n = 26) | 0.017 |
LLTS | ChC (cm) | 79.00 ± 8.02 b (n = 7) | 98.25 ± 1.61 a (n = 28) | 94.68 ± 2.55 a (n = 26) | 0.012 | |
MST2-S36 | HS | CaC (cm) | 7.19 ± 0.05 a (n = 110) | 6.99 ± 0.07 b (n = 76) | 7.10 ± 0.10 a b (n = 15) | 0.044 |
STHS | BoH (cm) | 63.76 ± 0.36 a (n = 137) | 62.27 ± 0.58 b (n = 46) | 60.39 ± 1.88 b (n = 7) | 0.022 | |
TS | CaC (cm) | 7.12 ± 0.11 b (n = 83) | 7.42 ± 0.11 a b (n = 68) | 7.69 ± 0.30 a (n = 15) | 0.039 |
Locus | Breed | Number | Genotypic Frequency | Allelic Frequency | Population Parameters | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
n | II | ID | DD | I | D | Ho | He | Ne | PIC | HWE | ||
YAP-S4 | LLTS | 61 | 0.016 | 0.246 | 0.738 | 0.139 | 0.861 | 0.246 | 0.239 | 1.315 | 0.211 | p > 0.05 |
STHS | 190 | 0.042 | 0.321 | 0.637 | 0.203 | 0.797 | 0.321 | 0.324 | 1.478 | 0.271 | p > 0.05 | |
HS | 201 | 0.040 | 0.303 | 0.657 | 0.192 | 0.808 | 0.303 | 0.310 | 1.450 | 0.262 | p > 0.05 | |
YAP-S5 | LLTS | 61 | 0.066 | 0.393 | 0.541 | 0.262 | 0.738 | 0.393 | 0.387 | 1.631 | 0.312 | p > 0.05 |
STHS | 190 | 0.042 | 0.342 | 0.616 | 0.213 | 0.787 | 0.342 | 0.335 | 1.504 | 0.279 | p > 0.05 | |
HS | 201 | 0.090 | 0.313 | 0.597 | 0.246 | 0.754 | 0.313 | 0.371 | 1.590 | 0.302 | p > 0.05 | |
YAP-S7 | LLTS | 61 | 0.738 | 0.246 | 0.016 | 0.861 | 0.139 | 0.246 | 0.239 | 1.315 | 0.211 | p > 0.05 |
STHS | 190 | 0.532 | 0.395 | 0.074 | 0.729 | 0.271 | 0.395 | 0.395 | 1.653 | 0.317 | p > 0.05 | |
HS | 201 | 0.647 | 0.308 | 0.045 | 0.801 | 0.199 | 0.308 | 0.319 | 1.468 | 0.268 | p > 0.05 | |
YAP-S11 | LLTS | 61 | 0.705 | 0.279 | 0.016 | 0.844 | 0.156 | 0.279 | 0.263 | 1.357 | 0.229 | p > 0.05 |
HS | 201 | 0.955 | 0.045 | 0.000 | 0.978 | 0.022 | 0.045 | 0.043 | 1.045 | 0.042 | p > 0.05 | |
YAP-S15 | LLTS | 61 | 0.656 | 0.328 | 0.016 | 0.820 | 0.180 | 0.328 | 0.295 | 1.419 | 0.252 | p > 0.05 |
STHS | 190 | 0.763 | 0.195 | 0.042 | 0.861 | 0.139 | 0.195 | 0.239 | 1.315 | 0.211 | p > 0.05 | |
YAP-S17 | LLTS | 61 | 0.541 | 0.410 | 0.049 | 0.746 | 0.254 | 0.410 | 0.379 | 1.610 | 0.307 | p > 0.05 |
STHS | 190 | 0.295 | 0.516 | 0.189 | 0.553 | 0.447 | 0.516 | 0.494 | 1.978 | 0.372 | p > 0.05 | |
TS | 166 | 0.795 | 0.181 | 0.024 | 0.886 | 0.114 | 0.181 | 0.202 | 1.253 | 0.182 | p > 0.05 | |
HS | 201 | 0.393 | 0.438 | 0.169 | 0.612 | 0.388 | 0.438 | 0.475 | 1.904 | 0.362 | p > 0.05 | |
YAP-S19 | LLTS | 61 | 0.803 | 0.197 | 0.000 | 0.902 | 0.098 | 0.197 | 0.177 | 1.215 | 0.161 | p > 0.05 |
STHS | 190 | 0.895 | 0.100 | 0.005 | 0.945 | 0.055 | 0.100 | 0.104 | 1.116 | 0.099 | p > 0.05 | |
TS | 166 | 0.693 | 0.277 | 0.030 | 0.831 | 0.169 | 0.277 | 0.281 | 1.391 | 0.241 | p > 0.05 | |
HS | 201 | 0.776 | 0.199 | 0.025 | 0.876 | 0.124 | 0.199 | 0.217 | 1.278 | 0.194 | p > 0.05 | |
YAP-S21 | LLTS | 61 | 0.607 | 0.344 | 0.049 | 0.779 | 0.221 | 0.344 | 0.344 | 1.525 | 0.285 | p > 0.05 |
STHS | 190 | 0.558 | 0.395 | 0.047 | 0.755 | 0.245 | 0.395 | 0.370 | 1.587 | 0.302 | p > 0.05 | |
TS | 166 | 0.614 | 0.349 | 0.036 | 0.789 | 0.211 | 0.349 | 0.333 | 1.499 | 0.278 | p > 0.05 | |
HS | 201 | 0.791 | 0.209 | 0.000 | 0.896 | 0.104 | 0.209 | 0.186 | 1.229 | 0.169 | p > 0.05 | |
TAZ-S17 | LLTS | 61 | 0.311 | 0.443 | 0.246 | 0.533 | 0.467 | 0.443 | 0.498 | 1.991 | 0.374 | p > 0.05 |
TS | 166 | 0.301 | 0.428 | 0.271 | 0.515 | 0.485 | 0.428 | 0.500 | 1.998 | 0.375 | p > 0.05 | |
TAZ-S24 | TS | 166 | 0.639 | 0.331 | 0.030 | 0.804 | 0.196 | 0.331 | 0.315 | 1.460 | 0.266 | p > 0.05 |
Locus | Breed | Growth Trait | Observed Genotypes (MEANS ± SEM) | p | ||
---|---|---|---|---|---|---|
II | ID | DD | ||||
YAP-S19 | LLTS | BoH (cm) | 75.43 ± 1.07 b (n = 49) | 85.25 ± 2.85 a (n = 12) | - | 0.005 |
LLTS | ChD (cm) | 34.95 ± 0.59 b (n = 49) | 38.88 ± 1.16 a (n = 12) | - | 0.035 | |
TS | BoL(cm) | 69.67 ± 0.53 b (n = 115) | 69.65 ± 0.92 b (n = 46) | 76.00 ± 1.53 a (n = 5) | 0.032 | |
YAP-S21 | LLTS | ChC (cm) | 97.35 ± 2.32 a (n = 37) | 94.70 ± 2.01 a (n = 21) | 80.17 ± 9.20 b (n = 3) | 0.028 |
STHS | ChC (cm) | 72.58 ± 0.52 a (n = 106) | 72.04 ± 0.78 a (n = 75) | 66.58 ± 1.38 b (n = 9) | 0.015 | |
TS | RuH (cm) | 49.28 ± 0.35 b (n = 102) | 50.80± 0.50 a (n = 58) | 49.67 ± 0.67 ab (n = 6) | 0.047 | |
TS | HeL (cm) | 19.88 ± 0.18 b (n = 102) | 20.86 ± 0.22 a (n = 58) | 19.50 ± 0.87 ab (n = 6) | 0.004 | |
TS | CaC (cm) | 7.11 ± 0.09 b (n = 102) | 7.60 ± 0.13 a (n = 58) | 7.93 ± 0.35 a (n = 6) | 0.004 |
Locus | Breed | Number | Genotypic Frequency | Allelic Frequency | Population Parameters | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
n | II | ID | DD | I | D | Ho | He | Ne | PIC | HWE | ||
MOB1A -S4 | LLTS | 61 | 0.754 | 0.230 | 0.016 | 0.869 | 0.131 | 0.230 | 0.228 | 1.295 | 0.202 | p > 0.05 |
STHS | 190 | 0.826 | 0.158 | 0.016 | 0.905 | 0.095 | 0.158 | 0.172 | 1.208 | 0.157 | p > 0.05 | |
MOB1A -S7 | LLTS | 61 | 0.623 | 0.361 | 0.016 | 0.803 | 0.197 | 0.361 | 0.316 | 1.463 | 0.266 | p > 0.05 |
STHS | 190 | 0.505 | 0.411 | 0.084 | 0.711 | 0.289 | 0.411 | 0.411 | 1.698 | 0.327 | p > 0.05 | |
TS | 166 | 0.530 | 0.361 | 0.108 | 0.711 | 0.289 | 0.361 | 0.411 | 1.698 | 0.327 | p > 0.05 | |
HS | 201 | 0.438 | 0.433 | 0.129 | 0.654 | 0.346 | 0.433 | 0.453 | 1.827 | 0.350 | p > 0.05 | |
MOB1A -S10 | LLTS | 61 | 0.738 | 0.213 | 0.049 | 0.844 | 0.156 | 0.213 | 0.263 | 1.357 | 0.229 | p > 0.05 |
STHS | 190 | 0.811 | 0.137 | 0.053 | 0.879 | 0.121 | 0.137 | 0.213 | 1.270 | 0.190 | p > 0.05 | |
HS | 201 | 0.821 | 0.144 | 0.035 | 0.893 | 0.107 | 0.144 | 0.191 | 1.236 | 0.173 | p > 0.05 |
Locus | Breed | Growth Trait | Observed Genotypes (MEANS ± SEM) | p | ||
---|---|---|---|---|---|---|
II | ID | DD | ||||
MOB1A-S4 | STHS | CaC (cm) | 7.11 ± 0.06 b (n = 157) | 6.95 ± 0.14 b (n = 30) | 8.13 ± 0.33 a (n = 3) | 0.027 |
MOB1A-S7 | HS | CaC (cm) | 6.96 ± 0.06 b (n = 88) | 7.20 ± 0.06 a (n = 87) | 7.31 ± 0.10 a (n = 26) | 0.002 |
HS | BW (kg) | 31.41 ± 0.49 b (n = 88) | 32.86 ± 0.48 a (n = 87) | 33.55 ± 0.84 a (n = 26) | 0.035 | |
TS | HiW (cm) | 17.52 ± 0.38 ab (n = 88) | 16.82 ± 0.36 b (n = 60) | 19.00 ± 0.57 a (n = 18) | 0.028 | |
TS | HeL(cm) | 20.34 ± 0.19 a (n = 88) | 19.73 ± 0.27 b (n = 60) | 20.75 ± 0.26 a (n = 18) | 0.046 | |
MOB1A-S10 | LLTS | CaC (cm) | 7.87 ± 0.17 a (n = 45) | 6.79 ± 0.32 b (n = 13) | 7.25 ± 0.25 ab (n = 3) | 0.024 |
STHS | ChC (cm) | 71.55 ± 0.46 b (n = 154) | 74.32 ± 0.49 a (n = 26) | 74.50 ± 0.39 a (n = 110) | 0.039 | |
HS | CaC (cm) | 7.05 ± 0.04 b (n = 165) | 7.31 ± 0.09 a (n = 29) | 7.50 ± 0.19 a (n = 7) | 0.011 |
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Zhang, B.; Zhao, W.; Tang, X.; Zhou, M.; Qiu, Y.; Wang, S.; Sun, X. Identification and Analysis of InDel Variants in Key Hippo Pathway Genes and Their Association with Growth Traits in Four Chinese Sheep Breeds. Vet. Sci. 2025, 12, 283. https://doi.org/10.3390/vetsci12030283
Zhang B, Zhao W, Tang X, Zhou M, Qiu Y, Wang S, Sun X. Identification and Analysis of InDel Variants in Key Hippo Pathway Genes and Their Association with Growth Traits in Four Chinese Sheep Breeds. Veterinary Sciences. 2025; 12(3):283. https://doi.org/10.3390/vetsci12030283
Chicago/Turabian StyleZhang, Beibei, Wanxia Zhao, Xiaoqin Tang, Meng Zhou, Yanbo Qiu, Shuhui Wang, and Xiuzhu Sun. 2025. "Identification and Analysis of InDel Variants in Key Hippo Pathway Genes and Their Association with Growth Traits in Four Chinese Sheep Breeds" Veterinary Sciences 12, no. 3: 283. https://doi.org/10.3390/vetsci12030283
APA StyleZhang, B., Zhao, W., Tang, X., Zhou, M., Qiu, Y., Wang, S., & Sun, X. (2025). Identification and Analysis of InDel Variants in Key Hippo Pathway Genes and Their Association with Growth Traits in Four Chinese Sheep Breeds. Veterinary Sciences, 12(3), 283. https://doi.org/10.3390/vetsci12030283