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Article

Study of the Diagnostic Value of Interleukin-6 and Interleukin-8 in Children with Acute Gastroenteritis

by
Maysaa El Sayed Zaki
1,
Mona Abdel Latif Alsayed
and
Raghdaa Shrief
*
1
Clinical Pathology Department, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt
2
Pediatric Department, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt
3
Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt
*
Author to whom correspondence should be addressed.
GERMS 2020, 10(1), 27-33; https://doi.org/10.18683/germs.2020.1182
Submission received: 10 October 2019 / Revised: 27 December 2019 / Accepted: 30 December 2019 / Published: 2 March 2020

Abstract

Introduction: Gastroenteritis in children is responsible for high morbidity and mortality. Our aim was to determine the serum and fecal levels of interleukin-6 (IL-6) and interleukin-8 (IL-8) in children with acute gastroenteritis of viral and bacterial etiology to assess their utility as diagnostic biomarkers for these infections. Methods: In this case-control study, the children were classified according to the pathogen recovered from the stool by bacterial culture or by direct viral antigen detection by enzyme immunoassay (EIA) into 50 children with acute bacterial gastroenteritis and 50 children with acute viral gastroenteritis. In addition, 50 apparently healthy children were included as a control group. Blood and stool samples were subjected to detection of IL-6 and IL-8. Results: There were statistically significant elevations of total leucocytes counts, absolute neutrophils count, C-reactive protein, serum IL-6 and serum IL-8 in children with gastroenteritis compared to healthy children (p < 0.001). CRP, serum IL-6 and IL-8 had significantly elevated levels in children with bacterial gastroenteritis compared to viral gastroenteritis. Fecal IL-6 and IL-8 had significantly elevated levels in children with acute gastroenteritis than in healthy control (p < 0.001). The area under the curve (AUC) showed that CRP and serum IL-6 could be used as discriminative markers for acute bacterial gastroenteritis in children, in comparison to serum IL-8. Conclusions: Elevated serum IL-6 and CRP can aid in differentiation between viral and bacterial gastroenteritis. Serum IL-8 had limited discrimination ability between viral and bacterial gastroenteritis. Stool levels of IL-6 and IL-8 were elevated in children with viral and bacterial gastroenteritis, however, their assessment by enzyme linked immunosorbent assay had technical limitations to be used as differentiation biomarkers.
Keywords: fecal IL-6; IL-8; bacterial gastroenteritis; AUC fecal IL-6; IL-8; bacterial gastroenteritis; AUC

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MDPI and ACS Style

Zaki, M.E.S.; Alsayed, M.A.L.; Shrief, R. Study of the Diagnostic Value of Interleukin-6 and Interleukin-8 in Children with Acute Gastroenteritis. GERMS 2020, 10, 27-33. https://doi.org/10.18683/germs.2020.1182

AMA Style

Zaki MES, Alsayed MAL, Shrief R. Study of the Diagnostic Value of Interleukin-6 and Interleukin-8 in Children with Acute Gastroenteritis. GERMS. 2020; 10(1):27-33. https://doi.org/10.18683/germs.2020.1182

Chicago/Turabian Style

Zaki, Maysaa El Sayed, Mona Abdel Latif Alsayed, and Raghdaa Shrief. 2020. "Study of the Diagnostic Value of Interleukin-6 and Interleukin-8 in Children with Acute Gastroenteritis" GERMS 10, no. 1: 27-33. https://doi.org/10.18683/germs.2020.1182

APA Style

Zaki, M. E. S., Alsayed, M. A. L., & Shrief, R. (2020). Study of the Diagnostic Value of Interleukin-6 and Interleukin-8 in Children with Acute Gastroenteritis. GERMS, 10(1), 27-33. https://doi.org/10.18683/germs.2020.1182

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