Tetracycline Induces the Formation of Biofilm of Bacteria from Different Phases of Wastewater Treatment
, Kateřina Malachová 1, Jaroslav Semerád 2,3, Meta Sterniša 4, Zuzana Rybková 1 and Sonja Smole Možina 4Round 1
Reviewer 1 Report
The article titled "Tetracycline Induces the Formation of Biofilm of 2 Bacteria from Different Phases of Wastewater 3 Treatment" by Tereza et al. monitored the effect of tetracycline on the bacterial biofilm formation. The manuscript is well arranged and the quality the figures is good. This can be a good contribution to the field. I recommend publication following some minor revisions.
Please improve the introduction.
Please reduce the number of citations. Also, do not include too many references in results and discussions.
Please proofread for minor language corrections.
Author Response
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Author Response.pdf
Reviewer 2 Report
The assessed article deals with the influence of tetracycline on the formation of bacterial biofilm in the process of wastewater treatment. Recently, the issue of antibiotics in wastewater has been at the forefront of interest. The spread of antibiotic resistance genes has an impact on human and animal health. In this article, the authors mapped the presence of resistance genes in WWTP, in nitrification tank and in the sedimentation tank. The statement that "resistant bacteria are able of forming a biofilm and sub-inhibitory tetracycline concentrations induce biofilm formation" is not a new information. It has been published in the scientifical literature many times. On the other hand, I must point out that the issue of antibiotic-resistant biofilm formation is often addressed with regard to the hospital environment and patient health, not from the point of view of wastewater treatment. This view brings new knowledge and expands existing knowledge in this area. I really appreciate the well-designed introduction and clear methodology of experiments. The article is written very carefully, is logically arranged and is supplemented by a large number of literary sources. Congratulations to the authors for writing a very interesting article that will contribute to the study of resistance gene transfer in the environment.
Author Response
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