Bergenin from Bergenia Species Produces a Protective Response against Myocardial Infarction in Rats^†

Round 1

Reviewer 1 Report

The manuscript by Ahmad and colleagues entitled "Bergenin from Bergenia species produce cardioprotective response against isoproterenol-induced myocardial infarction in rats" describe the cardioprotective effects of bergenin on isoproterenol-induced myocardial infarction in rats.  The research is quite interesting, however some revisions are necessary on figures : standardize the size of the figure and text of the captions.

Author Response

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Reviewer 2 Report

I reviewed the manuscript entitled, Bergenin from Bergenia species produce cardioprotective response against isoproterenol-induced myocardial infarction in rats. Originality of the work is satisfactory. The authors summarized the findings clearly and made an appropriate and robust discussion along with suitable literature comparison.

1.        Introduction should provide full details info on research gap and need of conducting this study with clear research objectives.

2.        Article has been well written although Conclusion need to be updated with study findings and future recommendations

3.        Are authors conducted any previous preliminary studies on bergenin against the isoproterenol in vitro studies? How do authors directly work on rats?

4.        Very few experiments

5.        Figures are well presented

6.        Line 105: tuckey should be Tuckey

7.        References are not according to journal format

Author Response

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Reviewer 3 Report

Comments to the Author

MANUSCRIPT DETAILS

Ms. Ref. No.: processes-1814601

Title: Bergenin from Bergenia species produce cardioprotective response against isoproterenol-induced myocardial infarction in rats

Article Type: Research Article

Journal: processes

GENERAL COMMENTS

The present study has been designed to evaluate the cardioprotective activity of bergenin in isoproterenol-induced myocardial damage in rats.

The interest in this manuscript is significant enough to merit publication.

My recommendation on submitted manuscript to the processes is to be accepted after major revisions.

The comments and questions provided below may help the authors to put the manuscript into better appropriate form for publication.

SPECIFIC COMMENTS

- Abstract

- Kindly specify clear and concise aim for your work, and the aim of evaluate the cardioprotective activity of bergenin in isoproterenol-induced myocardial damage in rats “Abstract”.

- Material and Methods

·       Please summarize the Response of bergenin against the isoproterenol-induced MI in rats in material and methods section 

·       Please explain "Analysis of cardiac biomarkers" in material and methods section 

- Result and discussion

·       Figure 2 not clear, what it means?

·       Design of groups is not clear please, make fig explain the groups  

·       Please discuss the histopathological section in more detail

·       what is the safety dose of bergenin?

References

Revise references to be formatted in accordance with processes Author Guidelines.

Therefore, I think this paper is acceptable for publication with major revision.

Author Response

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Author Response File: Author Response.pdf

Reviewer 4 Report

“Many medicinal plants and its phytochemical constituents have been investigated and reported for cardioprotective activity 40 through different signaling pathways” a single study? (Khan and Gilani, 2015).

-The study is original enough to contribute to science, but the introduction, discussion and conclusion part were insufficient for me.

-the fiction was insufficient, the previous studies were not sufficiently included in the discussions.

-What kind of changes does "bergenin" cause in cells, physiologically it would be more meaningful and contributing to the discussion.

Author Response

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Author Response File: Author Response.pdf

Round 2

Reviewer 2 Report

Authors are now answered the questions/suggestions raised by me. In my opinion, this version of the manuscript can be accepted for publication in this journal. 

Reviewer 3 Report

MANUSCRIPT DETAILS

Ms. Ref. No.: processes-1814601

Title: Bergenin from Bergenia species produce cardioprotective response against isoproterenol-induced myocardial infarction in rats

Article Type: Research Article

Journal: processes

GENERAL COMMENTS

The present study has been designed to evaluate the cardioprotective activity of bergenin in isoproterenol-induced myocardial damage in rats. 

The interest in this manuscript is significant enough to merit publication.

 Accept in present form

Reviewer 4 Report

good work