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Design and Engineering of Deimmunized Vaccinia Viral Vectors
Article

Fluorescent Tagged Vaccinia Virus Genome Allows Rapid and Efficient Measurement of Oncolytic Potential and Discovery of Oncolytic Modulators

1
NeoVirTech SAS, 31106 Toulouse, France
2
Transgene SA, 67405 Illkirch-Graffenstaden, France
3
Imactiv-3D SAS, 31106 Toulouse, France
4
Centre de Biologie Intégrative (CBI), Laboratoire de Biologie Moléculaire Eucaryote (LBME), University of Toulouse, UPS, CNRS, 31400 Toulouse, France
5
Institut Universitaire de France (IUF), 75231 Paris, France
6
IHAP, Université de Toulouse, INRA, ENVT, 31076 Toulouse, France
*
Authors to whom correspondence should be addressed.
Biomedicines 2020, 8(12), 543; https://doi.org/10.3390/biomedicines8120543
Received: 28 October 2020 / Revised: 22 November 2020 / Accepted: 24 November 2020 / Published: 26 November 2020
(This article belongs to the Special Issue Oncolytic Viruses as a Novel Form of Immunotherapy for Cancer II)
As a live biologic agent, oncolytic vaccinia virus has the ability to target and selectively amplify at tumor sites. We have previously reported that deletion of thymidine kinase and ribonucleotide reductase genes in vaccinia virus can increase the safety and efficacy of the virus. Here, to allow direct visualization of the viral genome in living cells, we incorporated the ANCH target sequence and the OR3-Santaka gene in the double-deleted vaccinia virus. Infection of human tumor cells with ANCHOR3-tagged vaccinia virus enables visualization and quantification of viral genome dynamics in living cells. The results show that the ANCHOR technology permits the measurement of the oncolytic potential of the double deleted vaccinia virus. Quantitative analysis of infection kinetics and of viral DNA replication allow rapid and efficient identification of inhibitors and activators of oncolytic activity. Our results highlight the potential application of the ANCHOR technology to track vaccinia virus and virtually any kind of poxvirus in living cells. View Full-Text
Keywords: oncolytic vaccinia virus; poxvirus modulators; fluorescence labeling; live cell imaging oncolytic vaccinia virus; poxvirus modulators; fluorescence labeling; live cell imaging
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MDPI and ACS Style

Gallardo, F.; Schmitt, D.; Brandely, R.; Brua, C.; Silvestre, N.; Findeli, A.; Foloppe, J.; Top, S.; Kappler-Gratias, S.; Quentin-Froignant, C.; Morin, R.; Lagarde, J.-M.; Bystricky, K.; Bertagnoli, S.; Erbs, P. Fluorescent Tagged Vaccinia Virus Genome Allows Rapid and Efficient Measurement of Oncolytic Potential and Discovery of Oncolytic Modulators. Biomedicines 2020, 8, 543. https://doi.org/10.3390/biomedicines8120543

AMA Style

Gallardo F, Schmitt D, Brandely R, Brua C, Silvestre N, Findeli A, Foloppe J, Top S, Kappler-Gratias S, Quentin-Froignant C, Morin R, Lagarde J-M, Bystricky K, Bertagnoli S, Erbs P. Fluorescent Tagged Vaccinia Virus Genome Allows Rapid and Efficient Measurement of Oncolytic Potential and Discovery of Oncolytic Modulators. Biomedicines. 2020; 8(12):543. https://doi.org/10.3390/biomedicines8120543

Chicago/Turabian Style

Gallardo, Franck, Doris Schmitt, Renée Brandely, Catherine Brua, Nathalie Silvestre, Annie Findeli, Johann Foloppe, Sokunthea Top, Sandrine Kappler-Gratias, Charlotte Quentin-Froignant, Renaud Morin, Jean-Michel Lagarde, Kerstin Bystricky, Stéphane Bertagnoli, and Philippe Erbs. 2020. "Fluorescent Tagged Vaccinia Virus Genome Allows Rapid and Efficient Measurement of Oncolytic Potential and Discovery of Oncolytic Modulators" Biomedicines 8, no. 12: 543. https://doi.org/10.3390/biomedicines8120543

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