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Biomedicines
Volume 13
Issue 8
10.3390/biomedicines13081941
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Co-Expression of TWIK-Related Acid-Sensitive K+ Channel 1 (TASK-1/KCNK3) and Platelet-Derived Growth Factor Receptor Alpha (PDGFRα/Pdgfra) in Adult Mouse Ovary

by
Byeonggyu Ahn
1,2,
Eun-Jin Kim
1,
Jin-Mok Kim
3,
Sohi Kang
2,4,
Sumi Hwang
5,
Chang-Woon Kim
6,
In Ae Cho
7,
Jeong Kyu Shin
7,
Eun-A Ko
8,
Dong-Kun Lee
1,2,* and
Dawon Kang
1,2,*
1
Department of Physiology, College of Medicine and Institute of Medical Sciences, Gyeongsang National University, Jinju 52727, Republic of Korea
2
Department of Convergence Medical Science, Gyeongsang National University, Jinju 52727, Republic of Korea
3
Department of Clinical Laboratory Science, Masan University, Changwon 2640, Republic of Korea
4
Department of Anatomy, College of Medicine, Institute of Medical Sciences, Gyeongsang National University, Jinju 52727, Republic of Korea
5
Department of Biomedical Laboratory Science, College of Health and Medical Science, Sangji University, Wonju 26339, Republic of Korea
6
Department of Obstetrics and Gynecology, Samsung Changwon Hospital, Sungkyunkwan University School of Medicine, Changwon 51353, Republic of Korea
7
Department of Obstetrics and Gynecology, College of Medicine, Gyeongsang National University, Jinju 52727, Republic of Korea
8
Department of Physiology, College of Medicine, Jeju National University, Jeju 63243, Republic of Korea
*
Authors to whom correspondence should be addressed.
Biomedicines 2025, 13(8), 1941; https://doi.org/10.3390/biomedicines13081941
Submission received: 5 June 2025 / Revised: 6 August 2025 / Accepted: 7 August 2025 / Published: 8 August 2025
(This article belongs to the Special Issue Ovarian Physiology and Reproduction)
Versions Notes

Abstract

Background/Objectives: Platelet-derived growth factor receptor alpha (PDGFRα) is a receptor involved in cell growth and differentiation, with unclear roles in ovarian tissues and potential interactions with KCNK3 (potassium two-pore domain channel subfamily K member 3), a member of the two-pore domain K+ channel involved in cellular homeostasis. This study aims to map PDGFRα expression across mouse tissues and to explore its co-expression with KCNK3 in the ovary. Methods: We visualized PDGFRα expression using RNA-seq data from the genotype-tissue expression (GTEx) BodyMAP across 54 human tissues and Cap Analysis of Gene Expression (CAGE) data for various mouse tissues. In PDGFRαEGFP mice expressing EGFP in PDGFRα+ cells, histological and fluorescence imaging were used to assess ovarian expression. Immunohistochemistry determined the co-localization of PDGFRα and KCNK3, and qPCR quantified their mRNA levels in the ovary, oviduct, and uterus. Results: PDGFRα showed high expression in human and mouse female reproductive tissues, particularly the ovary. In the PDGFRαEGFP mouse model, PDGFRα was primarily found in the thecal layer and stromal cells, not in granulosa cells or oocytes. Immunohistochemistry indicated that 90.2 ± 8.7% of PDGFRα+ cells expressed KCNK3 in the ovarian stroma. qPCR revealed lower PDGFRα and KCNK3 expression in the ovary compared to the oviduct and uterus. Conclusions: This study shows that PDGFRα is predominantly expressed in ovarian stromal and theca cells and is highly co-localized with KCNK3, suggesting a potential role for PDGFRα+ cells in ionic regulation and their possible involvement in follicular development and ovarian physiology.
Keywords: ovary; platelet-derived growth factor receptor alpha; potassium channel subfamily K member 3; theca cells ovary; platelet-derived growth factor receptor alpha; potassium channel subfamily K member 3; theca cells

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MDPI and ACS Style

Ahn, B.; Kim, E.-J.; Kim, J.-M.; Kang, S.; Hwang, S.; Kim, C.-W.; Cho, I.A.; Shin, J.K.; Ko, E.-A.; Lee, D.-K.; et al. Co-Expression of TWIK-Related Acid-Sensitive K+ Channel 1 (TASK-1/KCNK3) and Platelet-Derived Growth Factor Receptor Alpha (PDGFRα/Pdgfra) in Adult Mouse Ovary. Biomedicines 2025, 13, 1941. https://doi.org/10.3390/biomedicines13081941

AMA Style

Ahn B, Kim E-J, Kim J-M, Kang S, Hwang S, Kim C-W, Cho IA, Shin JK, Ko E-A, Lee D-K, et al. Co-Expression of TWIK-Related Acid-Sensitive K+ Channel 1 (TASK-1/KCNK3) and Platelet-Derived Growth Factor Receptor Alpha (PDGFRα/Pdgfra) in Adult Mouse Ovary. Biomedicines. 2025; 13(8):1941. https://doi.org/10.3390/biomedicines13081941

Chicago/Turabian Style

Ahn, Byeonggyu, Eun-Jin Kim, Jin-Mok Kim, Sohi Kang, Sumi Hwang, Chang-Woon Kim, In Ae Cho, Jeong Kyu Shin, Eun-A Ko, Dong-Kun Lee, and et al. 2025. "Co-Expression of TWIK-Related Acid-Sensitive K+ Channel 1 (TASK-1/KCNK3) and Platelet-Derived Growth Factor Receptor Alpha (PDGFRα/Pdgfra) in Adult Mouse Ovary" Biomedicines 13, no. 8: 1941. https://doi.org/10.3390/biomedicines13081941

APA Style

Ahn, B., Kim, E.-J., Kim, J.-M., Kang, S., Hwang, S., Kim, C.-W., Cho, I. A., Shin, J. K., Ko, E.-A., Lee, D.-K., & Kang, D. (2025). Co-Expression of TWIK-Related Acid-Sensitive K+ Channel 1 (TASK-1/KCNK3) and Platelet-Derived Growth Factor Receptor Alpha (PDGFRα/Pdgfra) in Adult Mouse Ovary. Biomedicines, 13(8), 1941. https://doi.org/10.3390/biomedicines13081941

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