The High-Resolution Structure Reveals Remarkable Similarity in PD-1 Binding of Cemiplimab and Dostarlimab, the FDA-Approved Antibodies for Cancer Immunotherapy
Abstract
1. Introduction
2. Materials and Methods
2.1. Expression and Purification of Proteins
2.2. Crystallization of the Complex
2.3. Data Collection and Structure Determination
3. Results
3.1. Crystal Structure of PD-1 in Complex with Cemiplimab
3.2. Specific Cemiplimab–PD-1 Interactions
3.3. Remarkably Similar Binding of Cemiplimab and Dostarlimab
3.4. Comprehensive Comparison of the PD-1 Binding by the Antibodies
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Data Collection | |
---|---|
X-ray source | PLS 5C |
Wavelength (Å) | 1.0000 |
Space group | P1 |
a, b, c (Å) | 55.06, 62.96, 81.29 |
α, β, γ (˚) | 105.73, 98.00, 92.44 |
Resolution (Å) | 1.98 (2.05–1.98) 1 |
Rmeas (%) | 7.20 (64.1) |
I/σI | 10.95 (2.21) |
Completeness (%) | 94.7 (94.6) |
Redundancy | 2.8 (2.5) |
CC1/2 | 0.998 (0.760) |
Refinement | |
Resolution (Å) | 1.98 |
No. of reflections | 68,386 |
Rwork/Rfree (%) | 18.7/23.5 |
No. atoms | |
Protein | 8210 |
Water | 644 |
Average B-factor (Å2) | 38.0 |
R.m.s. deviation | |
Bond lengths (Å) | 0.008 |
Bond angles (˚) | 1.039 |
Ramachandran | |
Favored (%) | 97.63 |
Allowed (%) | 2.37 |
Outlier (%) | 0.00 |
PDB code | 8GY5 |
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Jeong, T.-J.; Lee, H.-T.; Gu, N.; Jang, Y.-J.; Choi, S.-B.; Park, U.-B.; Lee, S.-H.; Heo, Y.-S. The High-Resolution Structure Reveals Remarkable Similarity in PD-1 Binding of Cemiplimab and Dostarlimab, the FDA-Approved Antibodies for Cancer Immunotherapy. Biomedicines 2022, 10, 3154. https://doi.org/10.3390/biomedicines10123154
Jeong T-J, Lee H-T, Gu N, Jang Y-J, Choi S-B, Park U-B, Lee S-H, Heo Y-S. The High-Resolution Structure Reveals Remarkable Similarity in PD-1 Binding of Cemiplimab and Dostarlimab, the FDA-Approved Antibodies for Cancer Immunotherapy. Biomedicines. 2022; 10(12):3154. https://doi.org/10.3390/biomedicines10123154
Chicago/Turabian StyleJeong, Tae-Jun, Hyun-Tae Lee, Nahyeon Gu, Yu-Jeong Jang, Seung-Beom Choi, Ui-Beom Park, Sang-Hyung Lee, and Yong-Seok Heo. 2022. "The High-Resolution Structure Reveals Remarkable Similarity in PD-1 Binding of Cemiplimab and Dostarlimab, the FDA-Approved Antibodies for Cancer Immunotherapy" Biomedicines 10, no. 12: 3154. https://doi.org/10.3390/biomedicines10123154
APA StyleJeong, T.-J., Lee, H.-T., Gu, N., Jang, Y.-J., Choi, S.-B., Park, U.-B., Lee, S.-H., & Heo, Y.-S. (2022). The High-Resolution Structure Reveals Remarkable Similarity in PD-1 Binding of Cemiplimab and Dostarlimab, the FDA-Approved Antibodies for Cancer Immunotherapy. Biomedicines, 10(12), 3154. https://doi.org/10.3390/biomedicines10123154