Calmodulin Gene Expression in Response to Mechanical Wounding and Botrytis cinerea Infection in Tomato Fruit
Abstract
:1. Introduction
2. Results
2.1. SlCaMs Are Responsive to Mechanical Wounding
2.2. Pathogen Infection Triggers Calmodulin Gene Expression
2.3. Calmodulin Genes Are Salicylic Acid-Responsive
2.4. Methyl Jasmonate Stimulates Calmodulin Gene Expression
2.5. Overexpression of the SlCaM2 Gene Reduces Symptoms Incited by Botrytis Cinerea
3. Discussion
4. Experimental Section
4.1. Plant Materials
4.2. Mechanical Injury, Methyl Jasmonate and Salicylic Acid Treatments
4.3. Pathogen Infection and Decay Assay
4.4. RNA Extraction and RT-qPCR
Primer Name | Oligonucleotides | Gene ID |
---|---|---|
SlCaM1-a | CCAGAGTTCCTTAACCTGATGG | Solyc01g008950 |
SlCaM1-b | CTTTTCGCCTAGGTTTGTCATC | |
SlCaM2-a | TCTGAGGAGGAGTTGAAAGAGG | Solyc10g081170 |
SlCaM2-b | TCAACATCAGCTTCCCTAATCA | |
SlCaM3-a | GATGGTAATGGAACCATCGACT | Solyc10g077010 |
SlCaM3-b | CATCAGTGAGCTTCTCACCAAG | |
SlCaM4-a | TCAGATCTCGGAGTTCAAAGAAG | Solyc11g072240 |
SlCaM4-b | CAGGTTAAGGAACTCAGGGAAGT | |
SlCaM5-a | TTAACTTGATGGCTCGGAAGAT | Solyc12g099990 |
SlCaM5-b | ACGAATCATCTCGTCAACCTCT | |
SlCaM6-a | ATCACTTGGTCAGAATCCCACT | Solyc03g098050 |
SlCaM6-b | AGCTGCAGAAATAAAGCCATTC | |
SlPR1-a | CTGTGAAGATGTGGGTTGATGAG | NM-001247429 |
SlPR1-b | TCTCCAGTTACCTGGTGGATCAT | |
SlPR2b-a | TCTTGCCCCATTTCAAGTTC | M80608 |
SlPR2b-b | TGCACGTGTATCCCTCAAAA | |
SlLAPA1-a | TGTCGCAGCATGTGAAAATATG | Solyc12g010020 |
SlLAPA1-b | AGCACCAGTTAATGTTGCCAGA | |
SlActin-a | GAAATAGCATAAGATGGCAGACG | X55749 |
SlActin-b | ATACCCACCATCACACCAGTAT | |
SlCaM2-S1 * | ggtggtaccATGGCGGATCAGCTGACGG | |
SlCaM2-S2 * | ggaggatccCTTGGCCATCATGACCTTAAC | |
SlCaM2-A1 * | ggtggtaccCTTGGCCATCATGACCTTAAC | |
SlCaM2-A2 * | ggaggatccATGGCGGATCAGCTGACGG |
4.5. Construction of Ti Plasmids Carrying Sense- and Antisense-SlCaM2 Gene
4.6. Fruit Agroinjection
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Peng, H.; Yang, T.; II, W.M.J. Calmodulin Gene Expression in Response to Mechanical Wounding and Botrytis cinerea Infection in Tomato Fruit. Plants 2014, 3, 427-441. https://doi.org/10.3390/plants3030427
Peng H, Yang T, II WMJ. Calmodulin Gene Expression in Response to Mechanical Wounding and Botrytis cinerea Infection in Tomato Fruit. Plants. 2014; 3(3):427-441. https://doi.org/10.3390/plants3030427
Chicago/Turabian StylePeng, Hui, Tianbao Yang, and Wayne M. Jurick II. 2014. "Calmodulin Gene Expression in Response to Mechanical Wounding and Botrytis cinerea Infection in Tomato Fruit" Plants 3, no. 3: 427-441. https://doi.org/10.3390/plants3030427