Comparative Determination of Phenolic Compounds in Arabidopsis thaliana Leaf Powder under Distinct Stress Conditions Using Fourier-Transform Infrared (FT-IR) and Near-Infrared (FT-NIR) Spectroscopy

Joshi, Rahul; Sathasivam, Ramaraj; Jayapal, Praveen Kumar; Patel, Ajay Kumar; Nguyen, Bao Van; Faqeerzada, Mohammad Akbar; Park, Sang Un; Lee, Seung Hyun; Kim, Moon S.; Baek, Insuck; Cho, Byoung-Kwan

doi:10.3390/plants11070836

Open AccessArticle

Comparative Determination of Phenolic Compounds in Arabidopsis thaliana Leaf Powder under Distinct Stress Conditions Using Fourier-Transform Infrared (FT-IR) and Near-Infrared (FT-NIR) Spectroscopy

by

Rahul Joshi

¹,

Ramaraj Sathasivam

²

,

Praveen Kumar Jayapal

¹

,

Ajay Kumar Patel

¹,

Bao Van Nguyen

²,

Mohammad Akbar Faqeerzada

¹

,

Sang Un Park

^2,3

,

Seung Hyun Lee

^1,3

,

Moon S. Kim

⁴,

Insuck Baek

⁴

and

Byoung-Kwan Cho

^1,3,*

¹

Department of Biosystems Machinery Engineering, College of Agricultural and Life Science, Chungnam National University, Daejeon 34134, Korea

²

Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea

³

Department of Smart Agriculture Systems, College of Agricultural and Life Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea

⁴

Environmental Microbial and Food Safety Laboratory, Agricultural Research Service, United States Department of Agriculture, Powder Mill Road, BARC-East, Bldg 303, Beltsville, MD 20705, USA

^*

Author to whom correspondence should be addressed.

Plants 2022, 11(7), 836; https://doi.org/10.3390/plants11070836

Submission received: 27 February 2022 / Revised: 14 March 2022 / Accepted: 19 March 2022 / Published: 22 March 2022

(This article belongs to the Special Issue Spectra Analysis and Plants Research)

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Abstract

The increasing interest in plant phenolic compounds in the past few years has become necessary because of their several important physicochemical properties. Thus, their identification through non-destructive methods has become crucial. This study carried out comparative non-destructive measurements of Arabidopsis thaliana leaf powder sample phenolic compounds using Fourier-transform infrared and near-infrared spectroscopic techniques under six distinct stress conditions. The prediction analysis of 600 leaf powder samples under different stress conditions (LED lights and drought) was performed using PLSR, PCR, and NAS-based HLA/GO regression analysis methods. The results obtained through FT-NIR spectroscopy yielded the highest correlation coefficient (

R_{p}^{2}

) value of 0.999, with a minimum error (RMSEP) value of 0.003 mg/g, based on the PLSR model using the MSC preprocessing method, which was slightly better than the correlation coefficient (

R_{p}^{2}

) value of 0.980 with an error (RMSEP) value of 0.055 mg/g for FT-IR spectroscopy. Additionally, beta coefficient plots present spectral differences and the identification of important spectral signatures sensitive to the phenolic compounds in the measured powdered samples. Thus, the obtained results demonstrated that FT-NIR spectroscopy combined with partial least squares regression (PLSR) and suitable preprocessing method has a solid potential for non-destructively predicting phenolic compounds in Arabidopsis thaliana leaf powder samples.

Keywords: Arabidopsis thaliana; phenolic compounds; Fourier-transform IR and NIR spectroscopy; non-destructive

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MDPI and ACS Style

Joshi, R.; Sathasivam, R.; Jayapal, P.K.; Patel, A.K.; Nguyen, B.V.; Faqeerzada, M.A.; Park, S.U.; Lee, S.H.; Kim, M.S.; Baek, I.; et al. Comparative Determination of Phenolic Compounds in Arabidopsis thaliana Leaf Powder under Distinct Stress Conditions Using Fourier-Transform Infrared (FT-IR) and Near-Infrared (FT-NIR) Spectroscopy. Plants 2022, 11, 836. https://doi.org/10.3390/plants11070836

AMA Style

Joshi R, Sathasivam R, Jayapal PK, Patel AK, Nguyen BV, Faqeerzada MA, Park SU, Lee SH, Kim MS, Baek I, et al. Comparative Determination of Phenolic Compounds in Arabidopsis thaliana Leaf Powder under Distinct Stress Conditions Using Fourier-Transform Infrared (FT-IR) and Near-Infrared (FT-NIR) Spectroscopy. Plants. 2022; 11(7):836. https://doi.org/10.3390/plants11070836

Chicago/Turabian Style

Joshi, Rahul, Ramaraj Sathasivam, Praveen Kumar Jayapal, Ajay Kumar Patel, Bao Van Nguyen, Mohammad Akbar Faqeerzada, Sang Un Park, Seung Hyun Lee, Moon S. Kim, Insuck Baek, and et al. 2022. "Comparative Determination of Phenolic Compounds in Arabidopsis thaliana Leaf Powder under Distinct Stress Conditions Using Fourier-Transform Infrared (FT-IR) and Near-Infrared (FT-NIR) Spectroscopy" Plants 11, no. 7: 836. https://doi.org/10.3390/plants11070836

APA Style

Joshi, R., Sathasivam, R., Jayapal, P. K., Patel, A. K., Nguyen, B. V., Faqeerzada, M. A., Park, S. U., Lee, S. H., Kim, M. S., Baek, I., & Cho, B.-K. (2022). Comparative Determination of Phenolic Compounds in Arabidopsis thaliana Leaf Powder under Distinct Stress Conditions Using Fourier-Transform Infrared (FT-IR) and Near-Infrared (FT-NIR) Spectroscopy. Plants, 11(7), 836. https://doi.org/10.3390/plants11070836

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Comparative Determination of Phenolic Compounds in Arabidopsis thaliana Leaf Powder under Distinct Stress Conditions Using Fourier-Transform Infrared (FT-IR) and Near-Infrared (FT-NIR) Spectroscopy

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