Roles for the RNA-Binding Protein Caper in Reproductive Output in Drosophila melanogaster
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fly Lines
2.2. Immunoblotting
2.3. Immunofluorescence
2.4. Fecundity Assay
2.5. Determining Fertilization and Embryonic Lethality
2.6. Larval and Pupal Lethality Assay
2.7. Mating Assay
2.8. Ovary Size Analysis
2.9. Statistical Analyses
3. Results
3.1. Caper Is Expressed during All Stages of Oogenesis
3.2. Caper Dysfunction Results in Lowered Reproductive Output for Females
3.3. Caper Dysfunction Results in Partial Embryonic and Larval Lethality
3.4. Caper Dysfunction Results in a Developmental Delay in Oogenesis
3.5. Reproductive Output of Caper−/−, but Not Caper Knockdown, Females May Be Impacted by Reduced Matings
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Males | Females | |||||||
---|---|---|---|---|---|---|---|---|
yw | caper−/− | RNAi Control | caper RNAi | yw | caper−/− | RNAi Control | caper RNAi | |
Larval Death | 3(63) | 5(46) | 0(46) | 3(43) | 0(59) | 9(50) | 2(53) | 2(49) |
Pupal Death | 6(60) | 8(41) | 2(46) | 1(40) | 8(59) | 3(41) | 4(51) | 1(47) |
Female Genotype | ||||||
---|---|---|---|---|---|---|
yw | caper−/− | yw; nosGal4 | nosGal4; UAScaperRNAi | Totals | ||
Male Genotype | yw | 6 | 0 | 2 | 4 | 270 |
caper−/− | 60 | 39 | 38 | 29 | 270 | |
Totals | 150 | 150 | 120 | 120 |
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Tixtha, E.J.; Super, M.K.; Titus, M.B.; Bono, J.M.; Olesnicky, E.C. Roles for the RNA-Binding Protein Caper in Reproductive Output in Drosophila melanogaster. J. Dev. Biol. 2023, 11, 2. https://doi.org/10.3390/jdb11010002
Tixtha EJ, Super MK, Titus MB, Bono JM, Olesnicky EC. Roles for the RNA-Binding Protein Caper in Reproductive Output in Drosophila melanogaster. Journal of Developmental Biology. 2023; 11(1):2. https://doi.org/10.3390/jdb11010002
Chicago/Turabian StyleTixtha, Erika J., Meg K. Super, M. Brandon Titus, Jeremy M. Bono, and Eugenia C. Olesnicky. 2023. "Roles for the RNA-Binding Protein Caper in Reproductive Output in Drosophila melanogaster" Journal of Developmental Biology 11, no. 1: 2. https://doi.org/10.3390/jdb11010002