Next Article in Journal
Stability-Indicating UPLC Method for Tramadol HCl Impurities in the Tramadol Injection after Dilution by Infusion Fluids (5% Dextrose and 0.9% Sodium Chloride)
Previous Article in Journal
Antimicrobial and Safety Properties of Lactobacilli Isolated from two Cameroonian Traditional Fermented Foods
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Sci. Pharm.
Volume 80
Issue 1
10.3797/scipharm.1111-13
scipharm-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
Scientia Pharmaceutica is published by MDPI from Volume 84 Issue 3 (2016). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Austrian Pharmaceutical Society (Österreichische Pharmazeutische Gesellschaft, ÖPhG).
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Screening and HPLC-Based Activity Profiling for New Antiprotozoal Leads from European Plants

by
Stefanie ZIMMERMANN
1,2,
Semira THOMI
1,
Marcel KAISER
2,
Matthias HAMBURGER
1 and
Michael ADAMS
1,*
1
Departement of Pharmaceutical Sciences, Pharmaceutical Biology, University of Basel, Klingelbergstrasse 50, 4056, Basel, Switzerland
2
Parasite Chemotherapy, Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Socinstrasse 57, 4002 Basel, Switzerland
*
Author to whom correspondence should be addressed.
Sci. Pharm. 2012, 80(1), 205-214; https://doi.org/10.3797/scipharm.1111-13
Submission received: 14 November 2011 / Accepted: 23 December 2011 / Published: 23 December 2011
Versions Notes

Abstract

Based on a survey of remedies used in Renaissance Europe to treat malaria, we prepared and screened a library of 254 extracts from 61 plants for antiplasmodial activity in vitro. HPLC-based activity profiling was performed for targeted identification of active constituents in extracts. One of the most remarkable results was the identification of onopordopicrin, a germacranolide sesquiterpene lactone isolated from Arctium nemorosum as a potent inhibitor of P. falciparum with an IC50 of 6.9 μM. It was tested similarly against Trypanosoma brucei rhodesiense, the parasite which causes African sleeping sickness. With an IC50 of 0.37 μM, onopordopicrin was one of the most potent natural products reported so far. Cytotoxicity was determined against rat myoblast L6 cells (IC50: 3.06).
Keywords: European plants; Plasmodium falciparum; Trypanosoma brucei rhodesiense; Arctium nemorosum; Onopordopicrin European plants; Plasmodium falciparum; Trypanosoma brucei rhodesiense; Arctium nemorosum; Onopordopicrin

Share and Cite

MDPI and ACS Style

ZIMMERMANN, S.; THOMI, S.; KAISER, M.; HAMBURGER, M.; ADAMS, M. Screening and HPLC-Based Activity Profiling for New Antiprotozoal Leads from European Plants. Sci. Pharm. 2012, 80, 205-214. https://doi.org/10.3797/scipharm.1111-13

AMA Style

ZIMMERMANN S, THOMI S, KAISER M, HAMBURGER M, ADAMS M. Screening and HPLC-Based Activity Profiling for New Antiprotozoal Leads from European Plants. Scientia Pharmaceutica. 2012; 80(1):205-214. https://doi.org/10.3797/scipharm.1111-13

Chicago/Turabian Style

ZIMMERMANN, Stefanie, Semira THOMI, Marcel KAISER, Matthias HAMBURGER, and Michael ADAMS. 2012. "Screening and HPLC-Based Activity Profiling for New Antiprotozoal Leads from European Plants" Scientia Pharmaceutica 80, no. 1: 205-214. https://doi.org/10.3797/scipharm.1111-13

APA Style

ZIMMERMANN, S., THOMI, S., KAISER, M., HAMBURGER, M., & ADAMS, M. (2012). Screening and HPLC-Based Activity Profiling for New Antiprotozoal Leads from European Plants. Scientia Pharmaceutica, 80(1), 205-214. https://doi.org/10.3797/scipharm.1111-13

Article Metrics

Back to TopTop