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Article

Cellular Interaction and Tumoral Penetration Properties of Cyclodextrin Nanoparticles on 3D Breast Tumor Model

1
Department of Nanotechnology and Nanomedicine, Hacettepe University, 06800 Ankara, Turkey
2
School of Pharmaceutical Sciences, University of Geneva-University of Lausanne, 1211 Geneva, Switzerland
3
Department of Pharmaceutical Technology, Faculty of Pharmacy, Hacettepe University, 06100 Ankara, Turkey
*
Author to whom correspondence should be addressed.
Nanomaterials 2018, 8(2), 67; https://doi.org/10.3390/nano8020067
Received: 1 December 2017 / Revised: 18 December 2017 / Accepted: 2 January 2018 / Published: 26 January 2018
(This article belongs to the Special Issue Pharmaceutical Nanotechnology)
Amphiphilic cyclodextrins are biocompatible oligosaccharides that can be used for drug delivery especially for the delivery of drugs with solubility problems thanks to their unique molecular structures. In this paper, Paclitaxel was used as a model anticancer drug to determine the inclusion complex properties of amphiphilic cyclodextrins with different surface charge. Paclitaxel-loaded cyclodextrin nanoparticles were characterized in terms of mean particle diameter, zeta potential, encapsulation efficacy, drug release profile and cell culture studies. It was determined that the nanoparticles prepared from the inclusion complex according to characterization studies have a longer release profile than the conventionally prepared nanoparticles. In order to mimic the tumor microenvironment, breast cancer cells and healthy fibroblast cells were used in 3-dimensional (3D) cell culture studies. It was determined that the activities of nanoparticles prepared by conventional methods behave differently in 2-dimensional (2D) and 3D cell cultures. In addition, it was observed that the nanoparticles prepared from the inclusion complex have a stronger anti-tumoral activity in the 3D multicellular tumor model than the drug solution. Furthermore, polycationic amphiphilic cyclodextrin nanoparticles can diffuse and penetrate through multilayer cells in a 3D tumor model, which is crucial for an eventual antitumor effect. View Full-Text
Keywords: 3D spheroid; amphiphilic cyclodextrin; cancer; cell culture; nanoparticles; Paclitaxel 3D spheroid; amphiphilic cyclodextrin; cancer; cell culture; nanoparticles; Paclitaxel
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MDPI and ACS Style

Varan, G.; Patrulea, V.; Borchard, G.; Bilensoy, E. Cellular Interaction and Tumoral Penetration Properties of Cyclodextrin Nanoparticles on 3D Breast Tumor Model. Nanomaterials 2018, 8, 67. https://doi.org/10.3390/nano8020067

AMA Style

Varan G, Patrulea V, Borchard G, Bilensoy E. Cellular Interaction and Tumoral Penetration Properties of Cyclodextrin Nanoparticles on 3D Breast Tumor Model. Nanomaterials. 2018; 8(2):67. https://doi.org/10.3390/nano8020067

Chicago/Turabian Style

Varan, Gamze, Viorica Patrulea, Gerrit Borchard, and Erem Bilensoy. 2018. "Cellular Interaction and Tumoral Penetration Properties of Cyclodextrin Nanoparticles on 3D Breast Tumor Model" Nanomaterials 8, no. 2: 67. https://doi.org/10.3390/nano8020067

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