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Open AccessArticle

Polydopamine/Transferrin Hybrid Nanoparticles for Targeted Cell-Killing

1
Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, Switzerland
2
Université de Strasbourg, Faculté de Chirurgie Dentaire, 8 Rue Sainte Elisabeth, 67000 Strasbourg, France
3
Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche 1121, 11 Rue Humann, 67085 Strasbourg CEDEX, France
*
Author to whom correspondence should be addressed.
Nanomaterials 2018, 8(12), 1065; https://doi.org/10.3390/nano8121065
Received: 21 November 2018 / Revised: 10 December 2018 / Accepted: 13 December 2018 / Published: 17 December 2018
(This article belongs to the Special Issue Nanoscience and Health: Tiny Technology Raises Big Questions)
Polydopamine can form biocompatible particles that convert light into heat. Recently, a protocol has been optimized to synthesize polydopamine/protein hybrid nanoparticles that retain the biological function of proteins, and combine it with the stimuli-induced heat generation of polydopamine. We have utilized this novel system to form polydopamine particles, containing transferrin (PDA/Tf). Mouse melanoma cells, which strongly express the transferrin receptor, were exposed to PDA/Tf nanoparticles (NPs) and, subsequently, were irradiated with a UV laser. The cell death rate was monitored in real-time. When irradiated, the melanoma cells exposed to PDA/Tf NPs underwent apoptosis, faster than the control cells, pointing towards the ability of PDA/Tf to mediate UV-light-induced cell death. The system was also validated in an organotypic, 3D-printed tumor spheroid model, comprising mouse melanoma cells, and the exposure and subsequent irradiation with UV-light, yielded similar results to the 2D cell culture. The process of apoptosis was found to be targeted and mediated by the lysosomal membrane permeabilization. Therefore, the herein presented polydopamine/protein NPs constitute a versatile and stable system for cancer cell-targeting and photothermal apoptosis induction. View Full-Text
Keywords: cell targeting; lysosomal membrane permeabilization; polydopamine/transferrin nanoparticles; live cell imaging; targeted apoptosis in vitro; 3D cell printing; spheroids cell targeting; lysosomal membrane permeabilization; polydopamine/transferrin nanoparticles; live cell imaging; targeted apoptosis in vitro; 3D cell printing; spheroids
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MDPI and ACS Style

Hauser, D.; Estermann, M.; Milosevic, A.; Steinmetz, L.; Vanhecke, D.; Septiadi, D.; Drasler, B.; Petri-Fink, A.; Ball, V.; Rothen-Rutishauser, B. Polydopamine/Transferrin Hybrid Nanoparticles for Targeted Cell-Killing. Nanomaterials 2018, 8, 1065.

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