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Bottom-Up Self-Assembly Based on DNA Nanotechnology

Effect of DNA Origami Nanostructures on hIAPP Aggregation

Technical and Macromolecular Chemistry, Paderborn University, Warburger Str. 100, 33098 Paderborn, Germany
Department of Chemistry, University of La Laguna, P.O. Box 456, E-38200 La Laguna, Spain
Author to whom correspondence should be addressed.
Nanomaterials 2020, 10(11), 2200;
Received: 22 September 2020 / Revised: 31 October 2020 / Accepted: 2 November 2020 / Published: 4 November 2020
(This article belongs to the Special Issue The Application of DNA Nanotechnology)
The aggregation of human islet amyloid polypeptide (hIAPP) plays a major role in the pathogenesis of type 2 diabetes mellitus (T2DM), and numerous strategies for controlling hIAPP aggregation have been investigated so far. In particular, several organic and inorganic nanoparticles (NPs) have shown the potential to influence the aggregation of hIAPP and other amyloidogenic proteins and peptides. In addition to conventional NPs, DNA nanostructures are receiving more and more attention from the biomedical field. Therefore, in this work, we investigated the effects of two different DNA origami nanostructures on hIAPP aggregation. To this end, we employed in situ turbidity measurements and ex situ atomic force microscopy (AFM). The turbidity measurements revealed a retarding effect of the DNA nanostructures on hIAPP aggregation, while the AFM results showed the co-aggregation of hIAPP with the DNA origami nanostructures into hybrid peptide–DNA aggregates. We assume that this was caused by strong electrostatic interactions between the negatively charged DNA origami nanostructures and the positively charged peptide. Most intriguingly, the influence of the DNA origami nanostructures on hIAPP aggregation differed from that of genomic double-stranded DNA (dsDNA) and appeared to depend on DNA origami superstructure. DNA origami nanostructures may thus represent a novel route for modulating amyloid aggregation in vivo. View Full-Text
Keywords: DNA origami; self-assembly; peptides; amyloid; atomic force microscopy DNA origami; self-assembly; peptides; amyloid; atomic force microscopy
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MDPI and ACS Style

Hanke, M.; Gonzalez Orive, A.; Grundmeier, G.; Keller, A. Effect of DNA Origami Nanostructures on hIAPP Aggregation. Nanomaterials 2020, 10, 2200.

AMA Style

Hanke M, Gonzalez Orive A, Grundmeier G, Keller A. Effect of DNA Origami Nanostructures on hIAPP Aggregation. Nanomaterials. 2020; 10(11):2200.

Chicago/Turabian Style

Hanke, Marcel, Alejandro Gonzalez Orive, Guido Grundmeier, and Adrian Keller. 2020. "Effect of DNA Origami Nanostructures on hIAPP Aggregation" Nanomaterials 10, no. 11: 2200.

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