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Open AccessArticle

How Molecular Weight Cut-Offs and Physicochemical Properties of Polyether Sulfone Membranes Affect Peptide Migration and Selectivity during Electrodialysis with Filtration Membranes

1
Institute of Nutrition and Functional Foods (INAF), Dairy Science and Technology Research Centre and Department of Food Sciences, Université Laval, Québec, QC G1V 0A6, Canada
2
Laboratory of Food Processing and ElectroMembrane Processes (LTAPEM), Université Laval, Québec, QC G1V 0A6, Canada
3
Amer-sil, Kehlen 8281, Luxemburg
*
Author to whom correspondence should be addressed.
Membranes 2019, 9(11), 153; https://doi.org/10.3390/membranes9110153
Received: 16 October 2019 / Revised: 6 November 2019 / Accepted: 7 November 2019 / Published: 13 November 2019
(This article belongs to the Special Issue Ion-Exchange Membranes and Processes)
Filtration membranes (FMs) are an integral part of electrodialysis with filtration membranes (EDFM), a green and promising technology for bioactive peptide fractionation. Therefore, it is paramount to understand how physicochemical properties of FMs impact global and selective peptide migration to anionic (ARC) and cationic (C+RC) peptide recovery compartments during their simultaneous separation by EDFM. In this context, six polyether sulfone (PES) membranes with molecular weight cut-offs (MWCO) of 5, 10, 20, 50, 100 and 300 kDa were characterized and used during EDFM to separate peptides from a complex whey protein hydrolysate. Surface charge, roughness, thickness and surface/pores nature of studied PES membranes were similar with small differences in conductivity, porosity and pore size distribution. Interestingly, global peptides migration to both recovery compartments increased linearly as a function of MWCO. However, peptide selectivity changed according to the recovery compartments and/or the peptide’s charge and MW with an increase in MWCO of FMs. Indeed, in ARC, the relative abundance (RA) of peptides having low negative charge and MW (IDALNENK and VLVLDTDYK) decreased (45% to 19%) with an increase in MWCO, while the opposite for peptides having high negative charge and MW (TPEVDDEALEK, TPEVDDEALEKFDK & VYVEELKPTPEGDLEILLQK) (increased from 16% to 43%). Concurrently, in C+RC, regardless of MWCO used, the highest RA was observed for peptides having low positive charge and MW (IPAVFK & ALPMHIR). It was the first time that the significant impact of charge, MWCO and pore size distribution of PES membranes on a wide range of MWCO was demonstrated on EDFM performances. View Full-Text
Keywords: electrodialysis with filtration membranes; membrane physicochemical properties; global peptide migration rate; peptide selectivity; molecular weight cut-offs electrodialysis with filtration membranes; membrane physicochemical properties; global peptide migration rate; peptide selectivity; molecular weight cut-offs
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MDPI and ACS Style

Kadel, S.; Pellerin, G.; Thibodeau, J.; Perreault, V.; Lainé, C.; Bazinet, L. How Molecular Weight Cut-Offs and Physicochemical Properties of Polyether Sulfone Membranes Affect Peptide Migration and Selectivity during Electrodialysis with Filtration Membranes. Membranes 2019, 9, 153.

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