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Antioxidants 2013, 2(4), 398-407;

Free Radical Scavenging and Antioxidant Activities of Silymarin Components

Department of Chemistry, Texas Southern University, Houston, TX 77004, USA
Author to whom correspondence should be addressed.
Received: 9 October 2013 / Revised: 13 November 2013 / Accepted: 21 November 2013 / Published: 10 December 2013
(This article belongs to the Special Issue Free Radicals)
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Silymarin is an over the counter food supplement that is sold as a liver enhancement and liver protection preparation. It is a major constituent of the seeds of Silybum marianum which is composed of a mixture of seven major components and several minor compounds. The seven major components: taxifolin, silychristin, silydianin, silybin A, silybin B, iso-silybin A and iso-silybin B were isolated and purified from the crude mixture of silymarin using preparative high performance liquid chromatography to determine which were the most effective for liver protection. Free radical scavenging, hydroxyl radical antioxidant capacity, oxygen radical antioxidant capacity, trolox-equivalent antioxidant capacity and total antioxidant capacity antioxidant activities were determined for each of the individual purified components as well as the crude silymarin mixture. Taxifolin was the most effective component for scavenging free radicals in the DPPH assay with an EC50 of 32 µM far more effective than all other components which showed EC50 ranging from 115 to 855 µM. Taxifolin was also found to be the most effective antioxidant in the oxygen radical antioxidant capacity (ORAC) assay with a trolox equivalent of 2.43 and the second most effective in the hydroxyl radical antioxidant capacity (HORAC) assay with a gallic acid equivalent of 0.57. Other antioxidants assays did not show significant differences between samples. View Full-Text
Keywords: Silybum marianum; milk thistle; food supplements; hepatoprotection Silybum marianum; milk thistle; food supplements; hepatoprotection

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Anthony, K.P.; Saleh, M.A. Free Radical Scavenging and Antioxidant Activities of Silymarin Components. Antioxidants 2013, 2, 398-407.

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