In the original publication [1], a mistake was identified in Figure 4 as published. The explanation in Figure 4E is wrong. The corrected Figure 4 appears below. The authors state that the scientific conclusions are unaffected. This correction was approved by the Academic Editor. The original publication has also been updated.
Figure 4.
Treatment with inhibitor M2 leads to the highest resistance to aflatoxin B1 in vitro. (A) Workflow of the structure-based virtual screening to identify inhibitors targeting BACH1. (B) Validation of the top three inhibitors (M1, M2, and M3) in Huh7 cells by CCK-8 assays. (C) Comparation of Huh7 tolerance to different AFB1 concentrations with and without M2 treatment. (D) The relative fluorescence intensity of AFB1-DNA adducts in Huh7 cells with and without M2 treatment. (E) Representative light microscopy images of AFB1-treated PK-15 cells with or without M2 treatment. Scale bar, 100 μm. (F) The IC50 assays for AFB1 in PK-15 cells with and without M2 treatment determined with CCK-8 assays. (G) The relative fluorescence intensity of AFB1-DNA adducts in PK-15 cells with and without M2 treatment. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant. p values were determined with two-tailed Student’s t-tests. AFB1, aflatoxin B1; M1, 1-Piperazineethanol, 4-phenyl-α-[[(3,4,5-trimethoxyphenyl)methoxy]methyl]; M2, 1-Piperazineethanol,α-[(1,3-benzodioxol-5-yloxy)methyl]-4-(2-methoxyphenyl); M3, 1,2-Ethanediamine, N1, N1, N2, N2-tetrakis (1H-benzimidazol-2-ylmethyl).
Reference
- Zhang, J.; Hu, S.; Zhao, C.; Zhou, Y.; Zhang, L.; Liu, H.; Zhou, P.; Li, S.; Fu, L.; Zheng, Z.; et al. Genome-Scale CRISPR Knockout Screening Identifies BACH1 as a Key Regulator of Aflatoxin B1-Induced Oxidative Damage. Antioxidants 2022, 11, 1787. [Google Scholar] [CrossRef] [PubMed]
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. |
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).