Next Article in Journal
Fabrication of Homogeneous High-Density Antibody Microarrays for Cytokine Detection
Previous Article in Journal
Particle-Based Microarrays of Oligonucleotides and Oligopeptides
Article Menu

Export Article

From the third issue of 2017, Microarrays has changed its name to High-Throughput.

Open AccessArticle

Expression Comparison of Oil Biosynthesis Genes in Oil Palm Mesocarp Tissue Using Custom Array

Sime Darby Technology Centre, Universiti Putra Malaysia, 1st Floor, Block B, UPM-MTDC Technology Centre III, Lebuh Silikon, 43400 Serdang, Selangor, Malaysia
European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SD, UK
School of Biosciences, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Malaysia
Department of Biological Sciences, Faculty of Science, National University of Singapore, Lower Kent Ridge Road, Singapore 117543, Singapore
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Microarrays 2014, 3(4), 263-281;
Received: 22 August 2014 / Revised: 22 September 2014 / Accepted: 3 November 2014 / Published: 13 November 2014
PDF [1808 KB, uploaded 13 November 2014]


Gene expression changes that occur during mesocarp development are a major research focus in oil palm research due to the economic importance of this tissue and the relatively rapid increase in lipid content to very high levels at fruit ripeness. Here, we report the development of a transcriptome-based 105,000-probe oil palm mesocarp microarray. The expression of genes involved in fatty acid (FA) and triacylglycerol (TAG) assembly, along with the tricarboxylic acid cycle (TCA) and glycolysis pathway at 16 Weeks After Anthesis (WAA) exhibited significantly higher signals compared to those obtained from a cross-species hybridization to the Arabidopsis (p-value < 0.01), and rice (p-value < 0.01) arrays. The oil palm microarray data also showed comparable correlation of expression (r2 = 0.569, p < 0.01) throughout mesocarp development to transcriptome (RNA sequencing) data, and improved correlation over quantitative real-time PCR (qPCR) (r2 = 0.721, p < 0.01) of the same RNA samples. The results confirm the advantage of the custom microarray over commercially available arrays derived from model species. We demonstrate the utility of this custom microarray to gain a better understanding of gene expression patterns in the oil palm mesocarp that may lead to increasing future oil yield. View Full-Text
Keywords: microarray; oil palm; mesocarp; gene expression microarray; oil palm; mesocarp; gene expression

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Wong, Y.C.; Kwong, Q.B.; Lee, H.L.; Ong, C.K.; Mayes, S.; Chew, F.T.; Appleton, D.R.; Kulaveerasingam, H. Expression Comparison of Oil Biosynthesis Genes in Oil Palm Mesocarp Tissue Using Custom Array. Microarrays 2014, 3, 263-281.

Show more citation formats Show less citations formats

Article Metrics

Article Access Statistics



[Return to top]
Microarrays EISSN 2076-3905 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top