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Particle-Based Microarrays of Oligonucleotides and Oligopeptides

Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany
German Cancer Research Center, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
Authors to whom correspondence should be addressed.
Microarrays 2014, 3(4), 245-262;
Received: 4 August 2014 / Revised: 15 October 2014 / Accepted: 16 October 2014 / Published: 28 October 2014
(This article belongs to the Special Issue New and Old Technologies for Generation of Microarrays)
In this review, we describe different methods of microarray fabrication based on the use of micro-particles/-beads and point out future tendencies in the development of particle-based arrays. First, we consider oligonucleotide bead arrays, where each bead is a carrier of one specific sequence of oligonucleotides. This bead-based array approach, appearing in the late 1990s, enabled high-throughput oligonucleotide analysis and had a large impact on genome research. Furthermore, we consider particle-based peptide array fabrication using combinatorial chemistry. In this approach, particles can directly participate in both the synthesis and the transfer of synthesized combinatorial molecules to a substrate. Subsequently, we describe in more detail the synthesis of peptide arrays with amino acid polymer particles, which imbed the amino acids inside their polymer matrix. By heating these particles, the polymer matrix is transformed into a highly viscous gel, and thereby, imbedded monomers are allowed to participate in the coupling reaction. Finally, we focus on combinatorial laser fusing of particles for the synthesis of high-density peptide arrays. This method combines the advantages of particles and combinatorial lithographic approaches. View Full-Text
Keywords: high-throughput; combinatorial synthesis; beads high-throughput; combinatorial synthesis; beads
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Nesterov-Mueller, A.; Maerkle, F.; Hahn, L.; Foertsch, T.; Schillo, S.; Bykovskaya, V.; Sedlmayr, M.; Weber, L.K.; Ridder, B.; Soehindrijo, M.; Muenster, B.; Striffler, J.; Bischoff, F.R.; Breitling, F.; Loeffler, F.F. Particle-Based Microarrays of Oligonucleotides and Oligopeptides. Microarrays 2014, 3, 245-262.

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