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• Denecke, B
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Microarrays 2013, 2(1), 24-33; doi:10.3390/microarrays2010024

Communication

Profiling Pre-MicroRNA and Mature MicroRNA Expressions Using a Single Microarray and Avoiding Separate Sample Preparation

Lin Gan and Bernd Denecke *

Interdisciplinary Centre for Clinical Research Aachen, RWTH Aachen University, Pauwelstr. 30, 52074 Aachen, Germany

* Author to whom correspondence should be addressed.

Received: 31 January 2013; in revised form: 26 February 2013 / Accepted: 12 March 2013 / Published: 14 March 2013

(This article belongs to the Special Issue MicroRNA Microarrays)

Download PDF Full-Text [261 KB, uploaded 14 March 2013 15:32 CET]

Abstract: Mature microRNA is a crucial component in the gene expression regulation network. At the same time, microRNA gene expression and procession is regulated in a precise and collaborated way. Pre-microRNAs mediate products during the microRNA transcription process, they can provide hints of microRNA gene expression regulation or can serve as alternative biomarkers. To date, little effort has been devoted to pre-microRNA expression profiling. In this study, three human and three mouse microRNA profile data sets, based on the Affymetrix miRNA 2.0 array, have been re-analyzed for both mature and pre-microRNA signals as a primary test of parallel mature/pre-microRNA expression profiling on a single platform. The results not only demonstrated a glimpse of pre-microRNA expression in human and mouse, but also the relationship of microRNA expressions between pre- and mature forms. The study also showed a possible application of currently available microRNA microarrays in profiling pre-microRNA expression in a time and cost effective manner.

Keywords: mature microRNA; pre-microRNA; profiling; microarray

Correction

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  A Correction was published on 24 June 2013 http://www.mdpi.com/2076-3905/2/3/170 (PDF, 33 KB)

  It came to our attention that a paper has recently been published concerning one of the GEO datasets (GSE34413) we cited in our published paper [1]. The original reference (reference 27) cited for this dataset leads to a paper about a similar study from the same research group [2]. In order to provide readers with exact citation information, we would like to update reference 27 in our previous paper to the new published paper concerning GSE34413 [3]. The authors apologize for this inconvenience.

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