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Challenges for MicroRNA Microarray Data Analysis

by Bin Wang 1 and Yaguang Xi 2,*
Department of Mathematics and Statistics, University of South Alabama, 411 University BLVD N,Room 325, Mobile, AL 36688, USA
Mitchell Cancer Institute, University of South Alabama, 1660 Springhill Avenue, Mobile, AL 36604,USA
Author to whom correspondence should be addressed.
Microarrays 2013, 2(2), 34-50;
Received: 20 February 2013 / Revised: 18 March 2013 / Accepted: 21 March 2013 / Published: 25 March 2013
(This article belongs to the Special Issue MicroRNA Microarrays)
Microarray is a high throughput discovery tool that has been broadly used for genomic research. Probe-target hybridization is the central concept of this technology to determine the relative abundance of nucleic acid sequences through fluorescence-based detection. In microarray experiments, variations of expression measurements can be attributed to many different sources that influence the stability and reproducibility of microarray platforms. Normalization is an essential step to reduce non-biological errors and to convert raw image data from multiple arrays (channels) to quality data for further analysis. In general, for the traditional microarray analysis, most established normalization methods are based on two assumptions: (1) the total number of target genes is large enough (>10,000); and (2) the expression level of the majority of genes is kept constant. However, microRNA (miRNA) arrays are usually spotted in low density, due to the fact that the total number of miRNAs is less than 2,000 and the majority of miRNAs are weakly or not expressed. As a result, normalization methods based on the above two assumptions are not applicable to miRNA profiling studies. In this review, we discuss a few representative microarray platforms on the market for miRNA profiling and compare the traditional methods with a few novel strategies specific for miRNA microarrays. View Full-Text
Keywords: microRNA; microarray; normalization; measurement error; qRT-PCR; bead array microRNA; microarray; normalization; measurement error; qRT-PCR; bead array
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Wang, B.; Xi, Y. Challenges for MicroRNA Microarray Data Analysis. Microarrays 2013, 2, 34-50.

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