The Application of Mesenchymal Stem Cells on Wound Repair and Regeneration
, PatrÃcia Sousa 1,2,†, Rui Alvites 1,2, Mariana Branquinho 1,2, Ana Sousa 1,2, Carla Mendonça 1,2, LuÃs Miguel Atayde 1,2 and Ana Colette MaurÃcio 1,2,*Round 1
Reviewer 1 Report
Refer to reviewer report
Comments for author File: 
Comments.docx
Author Response
Dear reviewer 1:
Thank you very much for the feedback on this review phase, and also for the suggestions made, which received the best attention from us. The changes made to the document are described below. All changes introduced appear in the final document highlighted in yellow. Please check our answers in the pdf in attachment.
Author Response File: 
Author Response.pdf
Reviewer 2 Report
Good review. I have one remark- "In matters of potency the only totipotent stem cells are the ones derived from the morula, being able to differentiate into every cell of the organism."- this information may be not actual. Check the research on mice- totipotency may be limited to as early as blastomeres stage.
Author Response
Dear reviewer 2:
Thank you very much for the feedback on this review phase, which received the best attention from us. All changes introduced appear in the final document highlighted in blue.
- “In matters of potency the only totipotent stem cells are the ones derived from the morula, being able to differentiate into evert cell of the organism.” This information may not be actual. Check the research on mice-totipotency may be limited to as early as blastomeres stage.
After some research, the authors confirmed that the written information in the paper is not actual.
“During early mammalian development, the totipotent state of early blastomeres is rapidly lost as cells gradually restrict their developmental potential and commit to distinct cell lineages by the blastocyst stage” [1].
“Extensive embryo manipulation experiments in mammalian embryos have been used to define the potency of individual blastomeres at different stages of development [2].“
“Finally, I propose that some mouse blastomeres, if not all, from a four-cell embryo, or from an early eight-cell embryo before its compaction are functionally and epigenetically totipotent cells. [3]“
For these reasons, the review paper was corrected, and the term “morula” changed to “blastomere” (Line 43) – changes in blue in the paper.
These were the references used in the search:
- Posfai, E., et al., 2020.
- Riveiro, A.R. and J.M. Brickman, From pluripotency to totipotency: an experimentalist's guide to cellular potency. Development, 2020. 147(16).
- Hu, K., On Mammalian Totipotency: What Is the Molecular Underpinning for the Totipotency of Zygote? Stem Cells Dev, 2019. 28(14): p. 897-906.
Author Response File: Author Response.pdf
Round 2
Reviewer 1 Report
The replies are satisfactory. The paper can be accepted in present form.
