Non-Thermal Atmospheric Pressure Argon-Sourced Plasma Flux Promotes Wound Healing of Burn Wounds and Burn Wounds with Infection in Mice through the Anti-Inflammatory Macrophages
, Kritsanawan Sae-Khow 1, Decho Thong-Aram 3 and Asada Leelahavanichkul 1,4,*Round 1
Reviewer 1 Report
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Comments for author File: 
Comments.pdf
Author Response
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Author Response File: Author Response.pdf
Reviewer 2 Report
The paper from Dang et al deals with the analysis of the effect of a cold (nonthermal) plasma treatment on burn wounds in mice.
The argument is of interest for the plasma-medicine community and the results proposed could help in understanding the possible future application of a plasma-based therapy for public health.
However, in my opinion, the paper needs revision in order to be ready for publication. Plasma medicine is a multidisciplinary field of research, relying on competences from a variety of scientists.
In the paper the parts related to the physics aspects of the experiment is too poorly reported. In particular throughout the various sections, a non-well defined “plasma energy”, “ionization energy” or similar forms are mentioned. Ionization energy refers to the quantity of energy that an atom in the ground electronic state must absorb to discharge an electron. It is not thus generally intended to be an energy source. A partially ionized plasma is made of both neutral and ionized atoms or molecules, eventually in excited states, and free electrons. The presence of these free-electron population at various thermal energy, along with the electromagnetic radiation emitted in the de-excitation process, are responsible for the generation of reactive chemical species, believed to be responsible for the plasma-induced effects.
I believed that sentences like “plasma energy”, “plasma can alter electrical charges”, “plasma flux initiates ionizing radiation”, “generating the temperatures”, “energy from ionization”, “penetration of the energy flux” and so on are too colloquial and not technically-scientifically sound.
Moreover, because of the variety of plasma sources used in the scientific literature and the associated variety of induced biological effects, I think that a much more detailed description of the plasma device must be given. The gas flow, the applied electric field, the geometry and material of both electrodes and of the dielectric material must be given in order to understand the electrical characteristics of the plasma source.
In Fig. 1c) and through section 3.1 the various applied voltages are said to lay within a few volts range, which, as obvious, as no-physical meaning.
How the experiments are performed? Which is the plasma source to substrate distance?
Few minor spelling and grammatical errors are present.
Figures are sufficiently clear, I would only suggest making them a little bigger.
Author Response
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Author Response File: Author Response.pdf
