Detection of Leishmania spp. in Cats: Analysis of Nasal, Oral and Conjunctival Swabs by PCR and HRM
Abstract
:Simple Summary
Abstract
1. Introduction
2. Material and Methods
2.1. Ethics Committee
2.2. Collection of Samples
2.3. Immunofluorescent Antibody Test—IFAT
2.4. Indirect ELISA Technique
2.5. DNA Extraction
2.6. Polymerase Chain Reaction and Sequencing
2.7. High-Resolution Melting (HRM)
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Polymerase Chain Reaction—PCR ITS1 and HSP70 Genes | ||||||||
---|---|---|---|---|---|---|---|---|
Identification | Clinical Signs | IFAT | ELISA | Conjunctival Swab | Oral Swab | Nasal Swab | Sequencing/ Accession Number | High-Resolution Melting—HRM |
Cat 10 | Skin lesions | Negative | Negative | Positive | Negative | Negative | - | - |
Cat 13 | Weight loss, apathy, skin lesions, alopecia, eye infection, and lymph node enlargement | Positive | Positive | Positive | Negative | Negative | L. donovani (98%)/JQ780821.1 | L. donovani |
Cat 15 | No signs | Positive | Positive | Positive | Negative | Negative | T. theileri (90%)/AB56920.1 | - |
Cat 26 | Weight loss, skin lesions, and alopecia | Positive | Positive | Positive | Negative | Negative | L. donovani (98%)/JQ780821.1 | L. donovani |
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Alves-Martin, M.F.; Bertozzo, T.V.; Aires, I.N.; Manzini, S.; Paixão-Marques, M.d.S.; Guiraldi, L.M.; dos Santos, W.J.; Sánchez, G.P.; Curci, V.C.L.M.; Richini-Pereira, V.B.; et al. Detection of Leishmania spp. in Cats: Analysis of Nasal, Oral and Conjunctival Swabs by PCR and HRM. Animals 2023, 13, 2468. https://doi.org/10.3390/ani13152468
Alves-Martin MF, Bertozzo TV, Aires IN, Manzini S, Paixão-Marques MdS, Guiraldi LM, dos Santos WJ, Sánchez GP, Curci VCLM, Richini-Pereira VB, et al. Detection of Leishmania spp. in Cats: Analysis of Nasal, Oral and Conjunctival Swabs by PCR and HRM. Animals. 2023; 13(15):2468. https://doi.org/10.3390/ani13152468
Chicago/Turabian StyleAlves-Martin, Maria Fernanda, Thainá Valente Bertozzo, Isabella Neves Aires, Suzane Manzini, Mirian dos Santos Paixão-Marques, Lívia Maísa Guiraldi, Wesley José dos Santos, Gabriela Pacheco Sánchez, Vera Cláudia Lorenzetti Magalhães Curci, Virgínia Bodelão Richini-Pereira, and et al. 2023. "Detection of Leishmania spp. in Cats: Analysis of Nasal, Oral and Conjunctival Swabs by PCR and HRM" Animals 13, no. 15: 2468. https://doi.org/10.3390/ani13152468
APA StyleAlves-Martin, M. F., Bertozzo, T. V., Aires, I. N., Manzini, S., Paixão-Marques, M. d. S., Guiraldi, L. M., dos Santos, W. J., Sánchez, G. P., Curci, V. C. L. M., Richini-Pereira, V. B., & Lucheis, S. B. (2023). Detection of Leishmania spp. in Cats: Analysis of Nasal, Oral and Conjunctival Swabs by PCR and HRM. Animals, 13(15), 2468. https://doi.org/10.3390/ani13152468