Presence of Porcine Circovirus Type 2 in the Environment of Farm Facilities without Pigs in Long Term-Vaccinated Farrow-to-Wean Farms
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Included Farms, PCV2 Surveillance and Environmental Sampling
Environmental Sample | Swabbing Protocol (One Swab Per Sample) |
---|---|
Sow feeder 1, piglet resting area 1, piglet hopper 2, weaning pen wall 2, gestation sow hopper. | In eight different elements of each type, a 25 × 25 centimetre area per element [26]. |
Sow crate 1. | The surface of lateral and rearward lower bars of eight different crates. |
Farrowing corridor 1, weaning pen floor 2, weaning corridor 2, gestation pen floor, gestation corridor, warehouse floor, office floor, parking area, farm main entrance, pig loading area. | 100 steps were taken on each surface wearing polyethylene boot covers, and then both boot covers were swabbed as indicated previously [13]: in zigzag from the toe region to the heel. |
Farrowing air fan 1, weaning air fan 2. | The surface of the fan blades or the protective grating |
Delivery management toolbox 1. | The whole ventral external surface, 50% of the internal surface and all the syringes included in it. |
Weaning pen railing 2. | 1 m in length in zigzag. |
Working utensils. | The handle of at least five different utensils (brushes, paddles…) for 10 s each. |
Feed wagons, pressure washer, sorting panel, office tables/chairs. | 50% of the surface. |
Door handles. | The surface of the exterior and the interior office door handles. |
Pens/Computer keyboard. | The surface of a computer keyboard or five different pens. |
Carcass container | The whole surface of the winch. |
Feed silo rungs. | The vertical and horizontal surfaces of five different rungs in at least two different silos. |
Farmer hands and hair/hat. | Hands: the dorsal and ventral surface of the hands, including each finger and the ventral surface of each fingernail. |
Workwear and streetwear. | The thorax area, the front and the back of each leg from the knee to the ankle, and the front and the back of each arm from the elbow to the wrist. |
Work boots and street boots. | In zigzag from the toe region to the heel. |
Farm staff vehicle. | A swab was rubbed on the door handle, the steering wheel, the gear shift lever, the handbrake, the pedals, the passenger seat and the dashboard |
2.2. Laboratory Analysis
3. Results
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- Segalés, J.; Allan, G.M.; Domingo, M. Porcine circovirus diseases. Anim. Health Res. Rev. 2005, 6, 119–142. [Google Scholar] [CrossRef] [PubMed]
- Alarcon, P.; Rushton, J.; Wieland, B. Cost of post-weaning multi-systemic wasting syndrome and porcine circovirus type-2 subclinical infection in England—An economic disease model. Prev. Vet. Med. 2013, 110, 88–102. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Segalés, J. Porcine circovirus type 2 (PCV2) infections: Clinical signs, pathology and laboratory diagnosis. Virus Res. 2012, 164, 10–19. [Google Scholar] [CrossRef]
- Tischer, I.; Gelderblom, H.; Vettermann, W.; Koch, M.A. A very small porcine virus with circular single-stranded DNA. Nature 1982, 295, 64–66. [Google Scholar] [CrossRef] [PubMed]
- Welch, J.; Bienek, C.; Gomperts, E.; Simmonds, P. Resistance of porcine circovirus and chicken anemia virus to virus inactivation procedures used for blood products. Transfusion 2006, 46, 1951–1958. [Google Scholar] [CrossRef] [PubMed]
- Kim, H.B.; Lyoo, K.S.; Joo, H.S. Disinfectants in vitro against porcine circovirus type 2. Vet. Rec. 2009, 164, 599–600. [Google Scholar] [CrossRef] [PubMed]
- Segalés, J. Best practice and future challenges for vaccination against porcine circovirus type 2. Expert Rev. Vaccines 2015, 14, 473–487. [Google Scholar] [CrossRef]
- Fraile, L.; Sarasola, P.; Sinovas, N.; Nofrarías, M.; López-Jimenez, R.; López-Soria, S.; Sibila, M.; Segalés, J. Inactivated PCV2 one shot vaccine applied in 3-week-old piglets: Improvement of production parameters and interaction with maternally derived immunity. Vaccine 2012, 30, 1986–1992. [Google Scholar] [CrossRef]
- Haake, M.; Palzer, A.; Rist, B.; Weissenbacher-Lang, C.; Fachinger, V.; Eggen, A.; Ritzmann, M.; Eddicks, M. Influence of age on the effectiveness of PCV2 vaccination in piglets with high levels of maternally derived antibodies. Vet. Microbiol. 2014, 168, 272–280. [Google Scholar] [CrossRef]
- Feng, H.; Segalés, J.; Fraile, L.; López-Soria, S.; Sibila, M. Effect of high and low levels of maternally derived antibodies on porcine circovirus type 2 (PCV2) infection dynamics and production parameters in PCV2 vaccinated pigs under field conditions. Vaccine 2016, 34, 3044–3050. [Google Scholar] [CrossRef]
- Afghah, Z.; Webb, B.; Meng, X.J.; Ramamoorthy, S. Ten years of PCV2 vaccines and vaccination: Is eradication a possibility? Vet. Microbiol. 2017, 206, 21–28. [Google Scholar] [CrossRef] [PubMed]
- Feng, H.; Blanco, G.; Segalés, J.; Sibila, M. Can Porcine circovirus type 2 (PCV2) infection be eradicated by mass vaccination? Vet. Microbiol. 2014, 172, 92–99. [Google Scholar] [CrossRef] [PubMed]
- Dee, S.; Deen, J.; Rossow, K.; Wiese, C.; Otake, S.; Joo, H.S.; Pijoan, C. Mechanical transmission of porcine reproductive and respiratory syndrome virus throughout a coordinated sequence of events during cold weather. Can. J. Vet. Res. 2002, 66, 232–239. [Google Scholar] [PubMed]
- Díaz-Cao, J.M.; Prieto, A.; López, G.; Fernández-Antonio, R.; Díaz, P.; López, C.; Remesar, S.; Díez-Baños, P.; Fernández, G. Molecular assessment of visitor personal protective equipment contamination with the Aleutian mink disease virus and porcine circovirus-2 in mink and porcine farms. PLoS ONE 2018, 13, e0203144. [Google Scholar] [CrossRef] [Green Version]
- Vigre, H.; Bækbo, P.; Jorsal, S.E.; Bille-Hansen, V.; Hassing, A.G.; Enøe, C.; Bøtner, A. Spatial and temporal patterns of pig herds diagnosed with Postweaning Multisystemic Wasting Syndrome (PMWS) during the first two years of its occurrence in Denmark. Vet. Microbiol. 2005, 110, 17–26. [Google Scholar] [CrossRef]
- Patterson, A.R.; Madson, D.M.; Schalk, S.D.; Halbur, P.G.; Opriessnig, T. Establishment and maintenance of a porcine circovirus type 2 (PCV2)-free breeding herd on a site that experienced a natural outbreak of PCV2-associated reproductive disease. J. Swine Health Prod. 2011, 19, 165–174. [Google Scholar]
- Horlen, K.P.; Dritz, S.S.; Nierfeld, J.C.; Henry, S.C.; Hesse, R.A.; Oberst, R.; Hays, M.; Anderson, J.; Rowland, R.R.R. A field evaluation of mortality rate and growth performance in pigs vaccinated against porcine circovirus type 2. J. Am. Vet. Med. Assoc. 2008, 232, 906–912. [Google Scholar] [CrossRef]
- Verreault, D.; Létourneau, V.; Gendron, L.; Masse, D.; Gagnon, C.A.; Duchaine, C. Airborne porcine circovirus in Canadian swine confinement buildings. Vet. Microbiol. 2010, 141, 224–230. [Google Scholar] [CrossRef]
- Dvorak, C.M.T.; Lilla, M.P.; Baker, S.R.; Murtaugh, M.P. Multiple routes of porcine circovirus type 2 transmission to piglets in the presence of maternal immunity. Vet. Microbiol. 2013, 166, 365–374. [Google Scholar] [CrossRef]
- López-Lorenzo, G.; Díaz-Cao, J.M.; Prieto, A.; López-Novo, C.; López, C.M.; Díaz, P.; Rodríguez-Vega, V.; Díez-Baños, P.; Fernández, G. Environmental distribution of Porcine Circovirus Type 2 (PCV2) in swine herds with natural infection. Sci. Rep. 2019, 9, 14816. [Google Scholar] [CrossRef] [Green Version]
- Anderson, B.D.; Yondon, M.; Bailey, E.S.; Duman, E.K.; Simmons, R.A.; Greer, A.G.; Gray, G.C. Environmental Bioaerosol Surveillance as an Early Warning System for Pathogen Detection in North Carolina Swine Farms: A Pilot Study. Transbound Emerg. Dis. 2021, 68, 361–367. [Google Scholar] [CrossRef] [PubMed]
- López-Lorenzo, G.; López-Novo, C.; Prieto, A.; Díaz, P.; Panadero, R.; Rodríguez-Vega, V.; Morrondo, P.; Fernández, G.; Díaz-Cao, J.M. Monitoring of porcine circovirus type 2 infection through air and surface samples in vaccinated and unvaccinated fattening farms. Transbound Emerg. Dis. 2022, 69, 1108–1117. [Google Scholar] [CrossRef] [PubMed]
- Real Decreto 306/2020 de 11 de Febrero, Por el Que se Establecen Normas Básicas de Ordenación de las Granjas Porcinas Intensivas, y se Modifica la Normativa Básica de Ordenación de las Explotaciones de Ganado Porcino Extensivo; España; pp. 13761–13791. Available online: https://www.boe.es/buscar/doc.php?id=BOE-A-2020-2110 (accessed on 8 December 2022).
- Prieto, A.; Díaz-Cao, J.; Fernández-Antonio, R.; Panadero, R.; Díaz, P.; López, C.; Morrondo, P.; Díez-Baños, P.; Fernández, G. Application of real-time PCR to detect Aleutian Mink Disease Virus on environmental farm sources. Vet. Microbiol. 2014, 173, 355–359. [Google Scholar] [CrossRef] [PubMed]
- Thrusfield, M. Surveys. In Veterinary Epidemiology; Thrusfield, M., Ed.; Blackwell Science: Oxford, UK, 2008; pp. 228–246. [Google Scholar]
- Espinosa-Gongora, C.; Larsen, J.; Moodley, A.; Nielsen, J.P.; Skov, R.L.; Andreasen, M.; Guardabassi, L. Farm-specific lineages of methicillin-resistant Staphylococcus aureus clonal complex 398 in Danish pig farms. Epidemiol. Infect. 2012, 140, 1794–1799. [Google Scholar] [CrossRef] [Green Version]
- Prieto, A.; Fernández-Antonio, R.; Díaz-Cao, J.M.; López, G.; Díaz, P.; Alonso, J.M.; Morrondo, P.; Fernández, G. Distribution of Aleutian mink disease virus contamination in the environment of infected mink farms. Vet. Microbiol. 2017, 204, 59–63. [Google Scholar] [CrossRef]
- O’Dea, M.A.; Hughes, A.P.; Davies, L.J.; Muhling, J.; Buddle, R.; Wilcox, G.E. Thermal stability of porcine circovirus type 2 in cell culture. J. Virol. Methods 2008, 147, 61–66. [Google Scholar] [CrossRef]
- Ruston, C.; Linhares, D.; Thomas, P.; Holtkamp, D. Pilot Study to Evaluate the Use of a Fluorescent Powder (Glo Germ) to Study the Transfer of Contamination from Livestock Trailers to the Center Alleyway and Pens in the Barn during Marketing Events; Project #19-147 SHIC; Iowa State University: Ames, IA, USA, 2019. [Google Scholar]
- Giacomini, E.; Gasparrini, S.; Lazzaro, M.; Scali, F.; Boniotti, M.B.; Corradi, A.; Pasquali, P.; Alborali, G.L. The role of transportation in the spread of Brachyspira hyodysenteriae in fattening farms. BMC Vet. Res. 2018, 14, 10. [Google Scholar] [CrossRef] [Green Version]
- Lowe, J.; Gauger, P.; Harmon, K.; Zhang, J.; Connor, J.; Yeske, P.; Loula, T.; Levis, I.; Dufresne, L.; Main, R. Role of transportation in spread of porcine epidemic diarrhea virus infection, United States. Emerg. Infect. Dis. 2014, 20, 872–874. [Google Scholar] [CrossRef]
- Thakur, K.K.; Revie, C.W.; Hurnik, D.; Poljak, Z.; Sanchez, J. Analysis of Swine Movement in Four Canadian Regions: Network Structure and Implications for Disease Spread. Transbound Emerg. Dis. 2016, 63, e14–e26. [Google Scholar] [CrossRef]
- Dieste-Pérez, L.; van Nes, A.; van Maanen, K.; Duinhof, T.; Tobias, T. The prevalence of PCV2 viremia in newborn piglets on four endemically infected Dutch sow farms is very low. Prev. Vet. Med. 2018, 153, 42–46. [Google Scholar] [CrossRef]
- Eddicks, M.; Koeppen, M.; Willi, S.; Fux, R.; Reese, S.; Sutter, G.; Stadler, J.; Ritzmann, M. Low prevalence of porcine circovirus type 2 infections in farrowing sows and corresponding pre-suckling piglets in southern German pig farms. Vet. Microbiol. 2016, 187, 70–74. [Google Scholar] [CrossRef] [PubMed]
- Eddicks, M.; Beuter, B.; Stuhldreier, R.; Nolte, T.; Reese, S.; Sutter, G.; Ritzmann, M.; Fux, R. Cross-sectional study on viraemia and shedding of porcine circovirus type 2 in a subclinically infected multiplier sow herd. Vet. Rec. 2018, 184, 189. [Google Scholar] [CrossRef] [PubMed]
- Pritchard, G.; Dennis, I.; Waddilove, J. Biosecurity: Reducing disease risks to pig breeding herds. Farm. Anim. Pract. 2005, 27, 230–237. [Google Scholar] [CrossRef]
- Laanen, M.; Persoons, D.; Ribbens, S.; de Jong, E.; Callens, B.; Strubbe, M.; Maes, D.; Dewulf, J. Relationship between biosecurity and production/antimicrobial treatment characteristics in pig herds. Vet. J. 2013, 198, 508–512. [Google Scholar] [CrossRef] [PubMed]
Farm | A | B | C | D |
---|---|---|---|---|
Number of sows | 360 | 200 | 300 | 300 |
Number of buildings | 3 | 1 | 1 | 1 |
Number of farrowing rooms | 7 | 6 | 6 | 6 |
Number of weaning rooms | 6 | 4 | 6 | 7 |
Number of gestation rooms | 1 | 1 | 1 | 1 |
PCV2 vaccination strategy | Only piglets at 4 weeks of age | Only piglets at 3 weeks of age | Only piglets at 3 weeks of age | Only piglets at 3 weeks of age |
Years of vaccinating against PCV2 | 7 | 7 | 8 | 8 |
PRRS infection | Negative | Negative | Negative | Positive |
Farm Facility | Sample | Farm N° of Environmental Samples (% of PCV2 Positive Samples) | |||
---|---|---|---|---|---|
A 33 (21.21%) | B 36 (22.22%) | C 36 (0%) | D 36 (19.44%) | ||
Farrowing area (piglets older than 10 days) | Sow feeder | - | - | - | - |
Sow crate | - | - | - | - | |
Piglet resting area | - | - | - | - | |
Corridor | - | - | - | - | |
Air fan | - | - | - | - | |
Delivery management toolbox | Not sampled * | - | - | - | |
Weaning area (piglets older than 8 weeks) | Piglet hopper | - | - | - | - |
Pen wall | - | - | - | - | |
Pen floor | - | - | - | - | |
Corridor | - | - | - | - | |
Pen railing | - | - | - | - | |
Air fan | - | - | - | - | |
Gestation area | Sow hopper | - | - | - | - |
Pen floor | 2.63 × 103 | - | - | - | |
Corridor | 1.74 × 105 | - | - | - | |
Warehouses | Floor | 9.55 × 103 | - | - | 5.01 × 104 |
Working utensils | - | - | - | - | |
Feed wagons | 1.02 × 103 | - | - | - | |
Pressure washer | Not sampled * | - | - | - | |
Sorting panels | - | - | - | - | |
Office | Floor | - | 7.94 × 102 | - | 1.20 × 107 |
Door handles | 1.45 × 103 | 9.77 × 102 | - | - | |
Pens/Computer keyboard | - | 7.08 × 102 | - | - | |
Tables/Chairs | - | 9.12 × 103 | - | - | |
Farm perimeter | Parking area | - | - | - | - |
Farm main entrance | - | - | - | 8.13 × 102 | |
Pig loading area | Not sampled * | - | - | 1.51 × 103 | |
Carcass container | - | 1.10 × 103 | - | - | |
Feed silo rungs | - | 6.46 × 102 | - | - | |
Farm staff | Hands | - | 1.02 × 103 | - | - |
Hair/hat | - | - | - | - | |
Workwear | - | 9.33 × 103 | - | - | |
Work boots | 1.66 × 104 | - | - | 1.95 × 107 | |
Streetwear | - | - | - | 5.89 × 102 | |
Street boots | 7.76 × 102 | - | - | 1.95 × 105 | |
Farm staff vehicle | - | - | - | - |
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. |
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
López-Lorenzo, G.; Prieto, A.; López-Novo, C.; Díaz, P.; Remesar, S.; Morrondo, P.; Fernández, G.; Díaz-Cao, J.M. Presence of Porcine Circovirus Type 2 in the Environment of Farm Facilities without Pigs in Long Term-Vaccinated Farrow-to-Wean Farms. Animals 2022, 12, 3515. https://doi.org/10.3390/ani12243515
López-Lorenzo G, Prieto A, López-Novo C, Díaz P, Remesar S, Morrondo P, Fernández G, Díaz-Cao JM. Presence of Porcine Circovirus Type 2 in the Environment of Farm Facilities without Pigs in Long Term-Vaccinated Farrow-to-Wean Farms. Animals. 2022; 12(24):3515. https://doi.org/10.3390/ani12243515
Chicago/Turabian StyleLópez-Lorenzo, Gonzalo, Alberto Prieto, Cynthia López-Novo, Pablo Díaz, Susana Remesar, Patrocinio Morrondo, Gonzalo Fernández, and José Manuel Díaz-Cao. 2022. "Presence of Porcine Circovirus Type 2 in the Environment of Farm Facilities without Pigs in Long Term-Vaccinated Farrow-to-Wean Farms" Animals 12, no. 24: 3515. https://doi.org/10.3390/ani12243515
APA StyleLópez-Lorenzo, G., Prieto, A., López-Novo, C., Díaz, P., Remesar, S., Morrondo, P., Fernández, G., & Díaz-Cao, J. M. (2022). Presence of Porcine Circovirus Type 2 in the Environment of Farm Facilities without Pigs in Long Term-Vaccinated Farrow-to-Wean Farms. Animals, 12(24), 3515. https://doi.org/10.3390/ani12243515