Next Article in Journal
Blood Profiling of Captive and Semi-Wild False Gharial in Peninsular Malaysia
Previous Article in Journal
The Effect of Horse Shoeing with Egg Bar Shoes and Shoes with Wedge Pads on the Results of Thermal Imaging of the Equine Distal Limb
Article

Three Manual Noncommercial Methods to Prepare Equine Platelet-Rich Plasma

1
Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana Champaign, Urbana, IL 61802, USA
2
School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu 18618681, SP, Brazil
3
Department of Comparative Biosciences, College of Veterinary Medicine, University of Illinois Urbana Champaign, Urbana, IL 61802, USA
4
Department of Pathobiology, College of Veterinary Medicine, University of Illinois Urbana-Champaign, Urbana, IL 61802, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally.
Current address: Ross University School of Veterinary Medicine, P.O. Box 334, Basseterre, St. Kitts, West Indies.
Academic Editor: Carmen Matás
Animals 2021, 11(6), 1478; https://doi.org/10.3390/ani11061478
Received: 25 April 2021 / Accepted: 10 May 2021 / Published: 21 May 2021
(This article belongs to the Section Animal Reproduction)
Platelet-rich plasma (PRP) is a popular therapy in human and veterinary medicine. This study compared three manual noncommercial methods to prepare PRP and its cooling. The three methods concentrated platelets and did not alter viability. Method 1 (i.e., involving double centrifugation) resulted in the greatest platelet concentrations, while method 3 (sedimentation) resulted in the lowest platelet concentration and greater contamination with white blood cells (WBC). Cooling increased platelet agglutination over time across methods and affected platelet viability in PRP obtained with method 3. It remains unknown whether the different methods and cooling would affect PRP’s clinical efficacy.
In light of PRP’s increasing popularity in veterinary practice, this study aimed to compare three manual methods to prepare and cool equine PRP. The blood of 18 clinically healthy mares was collected via venipuncture in a blood transfusion bag (method 1), blood tubes (method 2), and a syringe (method 3). In method 1, samples were double centrifuged; method 2 involved one centrifugation, and in method 3 the syringe was kept in an upright position to sediment for 4 h. After processing with three methods, PRP and platelet-poor plasma (PPP) were extracted and assessed for red (RBC) and white blood cell counts (WBC), platelet counts, and viability. In a subset of mares (n = 6), samples were processed with the three methods, and PRP was evaluated at 6 and 24 h postcooling at 5 °C. Method 1 resulted in the highest and method 3 in the lowest platelet concentration (p < 0.05), and the latter also had greater contamination with WBC than the others (p < 0.001). Platelet viability was similar across treatments (p > 0.05). Cooling for 24 h did not affect platelet counts in all methods (p > 0.05); however, platelet viability was reduced after cooling PRP produced by method 3 (p = 0.04), and agglutination increased over time in all methods (p < 0.001). The three methods increased (1.8–5.6-fold) platelet concentration in PRP compared to whole blood without compromising platelet viability. In conclusion, all three methods concentrated platelets and while cooling affected their viability. It remains unknown whether the different methods and cooling would affect PRP’s clinical efficacy. View Full-Text
Keywords: platelet concentrate; horse; endometritis; blood byproduct; platelet viability platelet concentrate; horse; endometritis; blood byproduct; platelet viability
Show Figures

Figure 1

MDPI and ACS Style

Segabinazzi, L.G.T.M.; Podico, G.; Rosser, M.F.; Nanjappa, S.G.; Alvarenga, M.A.; Canisso, I.F. Three Manual Noncommercial Methods to Prepare Equine Platelet-Rich Plasma. Animals 2021, 11, 1478. https://doi.org/10.3390/ani11061478

AMA Style

Segabinazzi LGTM, Podico G, Rosser MF, Nanjappa SG, Alvarenga MA, Canisso IF. Three Manual Noncommercial Methods to Prepare Equine Platelet-Rich Plasma. Animals. 2021; 11(6):1478. https://doi.org/10.3390/ani11061478

Chicago/Turabian Style

Segabinazzi, Lorenzo G.T.M., Giorgia Podico, Michael F. Rosser, Som G. Nanjappa, Marco A. Alvarenga, and Igor F. Canisso. 2021. "Three Manual Noncommercial Methods to Prepare Equine Platelet-Rich Plasma" Animals 11, no. 6: 1478. https://doi.org/10.3390/ani11061478

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop