Immunoregulatory Effects of Porcine Plasma Protein Concentrates on Rat Intestinal Epithelial Cells and Splenocytes
Abstract
Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Materials
2.3. Primary Splenocytes
2.4. IEC18 Experiments
2.5. Gene Silencing
2.6. Splenocyte Experiments
2.7. Isolation of Splenic T Lymphocytes and Monocytes
2.8. Statistical Analysis
3. Results
3.1. IC and SDS Induce Proinflammatory Cytokine Production in IEC18 Cells through a Pathway Involving the Activation of NF-κB
3.1.1. TLR4 Is Involved in the Induction of MCP-1 by SDS in IEC18 Cells
3.1.2. SDS and IC Regulate Cytokine Production in Spleen Cells
3.1.3. Signal Transduction Pathways Involving NF-κB, MAPK, AKT, or RAGE Do Not Contribute to Cytokine Regulation by IC or SPD in Spleen Cells
3.1.4. Induction of IL-10 Is Not Linked to TNF Inhibition by IC in Spleen Cells
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Hernández-Chirlaque, C.; Aranda, C.J.; Ocón, B.; Polo, J.; Martínez-Augustin, O.; Sánchez de Medina, F. Immunoregulatory Effects of Porcine Plasma Protein Concentrates on Rat Intestinal Epithelial Cells and Splenocytes. Animals 2021, 11, 807. https://doi.org/10.3390/ani11030807
Hernández-Chirlaque C, Aranda CJ, Ocón B, Polo J, Martínez-Augustin O, Sánchez de Medina F. Immunoregulatory Effects of Porcine Plasma Protein Concentrates on Rat Intestinal Epithelial Cells and Splenocytes. Animals. 2021; 11(3):807. https://doi.org/10.3390/ani11030807
Chicago/Turabian StyleHernández-Chirlaque, Cristina, Carlos J. Aranda, Borja Ocón, Javier Polo, Olga Martínez-Augustin, and Fermín Sánchez de Medina. 2021. "Immunoregulatory Effects of Porcine Plasma Protein Concentrates on Rat Intestinal Epithelial Cells and Splenocytes" Animals 11, no. 3: 807. https://doi.org/10.3390/ani11030807
APA StyleHernández-Chirlaque, C., Aranda, C. J., Ocón, B., Polo, J., Martínez-Augustin, O., & Sánchez de Medina, F. (2021). Immunoregulatory Effects of Porcine Plasma Protein Concentrates on Rat Intestinal Epithelial Cells and Splenocytes. Animals, 11(3), 807. https://doi.org/10.3390/ani11030807