First Birth of Cheetah Cubs from In Vitro Fertilization and Embryo Transfer
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals and Husbandry
2.2. Semen Collections
2.3. Hormonal Stimulations and Laparoscopic Oocyte Aspiration
2.4. Semen Preparation and In Vitro Fertilization
2.5. Embryo Culture and Laparoscopic Embryo Transfer
2.6. Pregnancy Monitoring Through Endocrinology and Ultrasonography
2.7. Genetic Testing
3. Results
3.1. Testicular Biometry and Semen Characteristics
3.2. Donor Response to the Stimulation and Oocyte Collection
3.3. Embryo Production and Transfer
3.4. Pregnancy Monitoring and Genetic Testing
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Semen Sample | Testicular Volume (cm3) | Raw Semen Metrics | |||
---|---|---|---|---|---|
Volume (mL) | Concentration (×106/mL) | Motile Sperm (%) | Normal Sperm (%) | ||
Sample 1 (male 9659) | 8.72 | 1.62 | 85.5 | 80 | 33 |
Sample 2 (male 9659) | 10.60 | 1.10 | 29.5 | 60 | 31 |
Sample 3 (male 9613) | 9.70 | 1.02 | 150.0 | 70 | 40 |
Donor and Recipient Females | Number of Ovarian Follicles/Female | Number of Ovulation Sites/Female | Number of Oocytes/Female * |
---|---|---|---|
Donor 1 | 18 | None | 18 |
Donor 2 | 8 | None | 12 |
Donor 3 | 20 | None | 18 |
Recipient 1 | 30 | None | N/A 1 |
Recipient 2 | 5 | 9 | N/A |
Recipient 3 | 11 | 3 | N/A |
Semen Sample | Concentration (x 106/mL) | Motile Sperm (%) | Total Sperm/IVF Drop | Donor Female |
---|---|---|---|---|
Sample 1 (male 9659) | 13.0 | 50 | 3.9 × 104 | Donor 1 |
Sample 2 (male 9659) | 12.5 | 80 | 5.0 × 104 | Donor 2 |
Sample 3 (male 9613) | 14.0 | 80 | 5.6 × 104 | Donor 3 |
Donor Females | Number of Cleaved Embryos (and %) on Day 1 Post-Insemination | Number of Cleaved Embryos (and %) on Day 2 Post-Insemination | Number of Transferred Embryos | Recipient Females |
---|---|---|---|---|
Donor 1 | 5 (27.8) | 5 (27.8) | 5 | Recipient 1 |
Donor 2 | 3 (25.0) | 5 (41.7) | 5 | Recipient 3 |
Donor 3 | 5 (27.8) | 10 (55.6) | 9 and 1 | Recipient 2 and 3 |
Offspring | Dam | Sire | Trio Typed Loci | Trio Loci Mismatches | Trio LOD Score |
---|---|---|---|---|---|
Cub 1 | Donor 3 | Sperm donor | 9 | 0 | 4.59 |
Recipient 2 | Sperm donor | 9 | 2 | −4.79 | |
Donor 3 | Unrelated male | 9 | 4 | −13.6 | |
Recipient 2 | Unrelated male | 9 | 6 | −23.9 | |
Cub 2 | Donor 3 | Sperm donor | 9 | 0 | 3.86 |
Recipient 2 | Sperm donor | 9 | 3 | −9.84 | |
Donor 3 | Unrelated male | 9 | 5 | −20.6 | |
Recipient 2 | Unrelated male | 9 | 6 | −22.6 |
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Crosier, A.E.; Lamy, J.; Bapodra, P.; Rapp, S.; Maly, M.; Junge, R.; Haefele, H.; Ahistus, J.; Santiestevan, J.; Comizzoli, P. First Birth of Cheetah Cubs from In Vitro Fertilization and Embryo Transfer. Animals 2020, 10, 1811. https://doi.org/10.3390/ani10101811
Crosier AE, Lamy J, Bapodra P, Rapp S, Maly M, Junge R, Haefele H, Ahistus J, Santiestevan J, Comizzoli P. First Birth of Cheetah Cubs from In Vitro Fertilization and Embryo Transfer. Animals. 2020; 10(10):1811. https://doi.org/10.3390/ani10101811
Chicago/Turabian StyleCrosier, Adrienne E., Julie Lamy, Priya Bapodra, Suzi Rapp, Morgan Maly, Randy Junge, Holly Haefele, Jason Ahistus, Jenny Santiestevan, and Pierre Comizzoli. 2020. "First Birth of Cheetah Cubs from In Vitro Fertilization and Embryo Transfer" Animals 10, no. 10: 1811. https://doi.org/10.3390/ani10101811