Next Article in Journal
Witch Hazel Significantly Improves the Efficacy of Commercially Available Teat Dips
Next Article in Special Issue
Identification of a Neisseria gonorrhoeae Histone Deacetylase: Epigenetic Impact on Host Gene Expression
Previous Article in Journal
Isolation of Naegleria spp. from a Brazilian Water Source
Previous Article in Special Issue
Atypical, Yet Not Infrequent, Infections with Neisseria Species
Open AccessReview

The Laboratory Diagnosis of Neisseria gonorrhoeae: Current Testing and Future Demands

1
Department of Dermatology, Venerology and Allergology, St. Josef Hospital, Ruhr-University, 44791 Bochum, Germany
2
German Consiliary Laboratory for Gonococci, Department of Dermatology and Venerology, Vivantes Hospital Berlin, 12351 Berlin, Germany
*
Author to whom correspondence should be addressed.
Pathogens 2020, 9(2), 91; https://doi.org/10.3390/pathogens9020091
Received: 7 January 2020 / Revised: 28 January 2020 / Accepted: 29 January 2020 / Published: 31 January 2020
(This article belongs to the Special Issue Neisseria gonorrhoeae Infections)
The ideal laboratory test to detect Neisseria gonorrhoeae (Ng) should be sensitive, specific, easy to use, rapid, and affordable and should provide information about susceptibility to antimicrobial drugs. Currently, such a test is not available and presumably will not be in the near future. Thus, diagnosis of gonococcal infections presently includes application of different techniques to address these requirements. Microscopy may produce rapid results but lacks sensitivity in many cases (except symptomatic urogenital infections in males). Highest sensitivity to detect Ng was shown for nucleic acid amplification technologies (NAATs), which, however, are less specific than culture. In addition, comprehensive analysis of antibiotic resistance is accomplished only by in vitro antimicrobial susceptibility testing of cultured isolates. As a light at the end of the tunnel, new developments of molecular techniques and microfluidic systems represent promising opportunities to design point-of-care tests for rapid detection of Ng with high sensitivity and specificity, and there is reason to hope that such tests may also provide antimicrobial resistance data in the future. View Full-Text
Keywords: gonorrhea; diagnostic; microscopy; culture; antimicrobial resistance; NAAT; point-of-care test; microfluidic gonorrhea; diagnostic; microscopy; culture; antimicrobial resistance; NAAT; point-of-care test; microfluidic
Show Figures

Graphical abstract

MDPI and ACS Style

Meyer, T.; Buder, S. The Laboratory Diagnosis of Neisseria gonorrhoeae: Current Testing and Future Demands. Pathogens 2020, 9, 91.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop