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Open AccessArticle

Isolation of Naegleria spp. from a Brazilian Water Source

Instituto de Física de São Carlos, Universidade de São Paulo, Caixa Postal 369, São Carlos 13560-590, SP, Brazil
Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, Avda. Astrofísico Fco. Sánchez, S/N, 38203 La Laguna, Tenerife, Canary Islands, Spain
Departamento de Ecologia e Biologia Evolutiva, Universidade Federal de São Carlos, Rodovia Washington Luis, km 235, CEP, São Carlos 13565-905, SP, Brazil
Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos 13560-590, Brazil
Author to whom correspondence should be addressed.
Pathogens 2020, 9(2), 90;
Received: 2 December 2019 / Revised: 23 January 2020 / Accepted: 28 January 2020 / Published: 31 January 2020
(This article belongs to the Special Issue Emerging Parasitic Protozoa)
The genus Naegleria, of the free-living amoeba (FLA) group, has been investigated mainly due to its human health impact, resulting in deadly infections and their worldwide distribution on freshwater systems. Naegleria fowleri, colloquially known as the “brain-eating amoeba,” is the most studied Naegleria species because it causes primary amoebic meningoencephalitis (PAM) of high lethality. The assessment of FLA biodiversity is fundamental to evaluate the presence of pathogenic species and the possibility of human contamination. However, the knowledge of FLA distribution in Brazil is unknown, and to rectify this situation, we present research on identifying Naegleria spp. in the Monjolinho River as a model study. The river is a public Brazilian freshwater source that crosses the city of São Carlos, in São Paulo state, Brazil. Five distinct sampling sites were examined through limnological features, trophozoites culturing, and PCR against internal transcribed spacer (ITS) regions and 5.8S rRNA sequences. The results identified N. philippinensis, N. canariensisi, N. australiensis, N. gruberi, N. dobsoni sequences, as well as a Hartmannella sequence. The methodology delineated here represents the first Brazilian Naegleria spp. study on a freshwater system. Our results stress the urgency of a large scale evaluation of the presence of free-living amoebas in Brazil.
Keywords: Naegleria spp.; free-living amoeba; PCR; Monjolinho River; Brazil Naegleria spp.; free-living amoeba; PCR; Monjolinho River; Brazil
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MDPI and ACS Style

Bellini, N.K.; Fonseca, A.L.M.; Reyes-Batlle, M.; Lorenzo-Morales, J.; Rocha, O.; Thiemann, O.H. Isolation of Naegleria spp. from a Brazilian Water Source. Pathogens 2020, 9, 90.

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