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Open AccessArticle

Intracellular Behaviour of Three Legionella pneumophila Strains within Three Amoeba Strains, Including Willaertia magna C2c Maky

1
Microbes, Evolution, Phylogeny and Infection department, Institut de Recherche pour le Développement IRD 198, Institut Hospitalo-Universitaire (IHU), Aix-Marseille Université, 13007 Marseille, France
2
R&D department, Amoéba, 38 Avenue des Frères Montgolfier, 69680 Chassieu, France
*
Author to whom correspondence should be addressed.
Pathogens 2020, 9(2), 105; https://doi.org/10.3390/pathogens9020105
Received: 10 January 2020 / Revised: 4 February 2020 / Accepted: 5 February 2020 / Published: 6 February 2020
(This article belongs to the Special Issue Legionella Contamination in Water Environment)
Legionella pneumophila is a facultative intracellular pathogen found in aquatic environments as planktonic cells within biofilms and as intracellular parasites of free-living amoebae such as Acanthamoeba castellanii. This pathogen bypasses the elimination mechanism to replicate within amoebae; however, not all amoeba species support the growth of L. pneumophila. Willaertia magna C2c Maky, a non-pathogenic amoeba, was previously demonstrated to possess the ability to eliminate the L. pneumophila strain Paris. Here, we study the intracellular behaviour of three L. pneumophila strains (Paris, Philadelphia, and Lens) within W. magna C2c Maky and compare this strain to A. castellanii and W. magna Z503, which are used as controls. We observe the intracellular growth of strain Lens within W. magna Z503 and A. castellanii at 22 °C and 37 °C. Strain Paris grows within A. castellanii at any temperature, while it only grows at 22 °C within W. magna Z503. Strain Philadelphia proliferates only within A. castellanii at 37 °C. Within W. magna C2c Maky, none of the three legionella strains exhibit intracellular growth. Additionally, the ability of W. magna C2c Maky to decrease the number of internalized L. pneumophila is confirmed. These results support the idea that W. magna C2c Maky possesses unique behaviour in regard to L. pneumophila strains.
Keywords: free-living amoebae; Legionella; biological biocide; cooling towers free-living amoebae; Legionella; biological biocide; cooling towers
MDPI and ACS Style

Hasni, I.; Jarry, A.; Quelard, B.; Carlino, A.; Eberst, J.-B.; Abbe, O.; Demanèche, S. Intracellular Behaviour of Three Legionella pneumophila Strains within Three Amoeba Strains, Including Willaertia magna C2c Maky. Pathogens 2020, 9, 105.

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