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Identification, Characterization and Expression Analysis of TRP Channel Genes in the Vegetable Pest, Pieris rapae

Ministry of Agricultural and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China
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Insects 2020, 11(3), 192; https://doi.org/10.3390/insects11030192
Received: 16 February 2020 / Revised: 12 March 2020 / Accepted: 17 March 2020 / Published: 18 March 2020
Transient receptor potential (TRP) channels are critical for insects to detect environmental stimuli and regulate homeostasis. Moreover, this superfamily has become potential molecular targets for insecticides or repellents. Pieris rapae is one of the most common and widely spread pests of Brassicaceae plants. Therefore, it is necessary to study TRP channels (TRPs) in P. rapae. In this study, we identified 14 TRPs in P. rapae, including two Water witch (Wtrw) genes. By contrast, only one Wtrw gene exists in Drosophila and functions in hygrosensation. We also found splice isoforms of Pyrexia (Pyx), TRPgamma (TRPγ) and TRP-Melastatin (TRPM). These three genes are related to temperature and gravity sensation, fine motor control, homeostasis regulation of Mg2+ and Zn2+ in Drosophila, respectively. Evolutionary analysis showed that the TRPs of P. rapae were well clustered into their own subfamilies. Real-time quantitative PCR (qPCR) showed that PrTRPs were widely distributed in the external sensory organs, including antennae, mouthparts, legs, wings and in the internal physiological organs, including brains, fat bodies, guts, Malpighian tubules, ovaries, as well as testis. Our study established a solid foundation for functional studies of TRP channels in P. rapae, and would be benefit to developing new approaches to control P. rapae targeting these important ion channels. View Full-Text
Keywords: Pieris rapae; transient receptor potential channel; splice isoform; gene duplication Pieris rapae; transient receptor potential channel; splice isoform; gene duplication
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Mao, F.; Lu, W.-J.; Yang, Y.; Qiao, X.; Ye, G.-Y.; Huang, J. Identification, Characterization and Expression Analysis of TRP Channel Genes in the Vegetable Pest, Pieris rapae. Insects 2020, 11, 192.

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