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Article

MicroRNA Expression Profile Changes after Cardiopulmonary Bypass and Ischemia/Reperfusion-Injury in a Porcine Model of Cardioplegic Arrest

1
Ludwig Boltzmann Institute for Cardiovascular Research at Center for Biomedical Research, Medical University of Vienna, 1090 Vienna, Austria
2
Institute of Physiology, Center for Physiology and Pharmacology, Medical University of Vienna, 1090 Vienna, Austria
3
TAmiRNA GmbH, 1110 Vienna, Austria
4
Division of Physiological Chemistry, Otto Loewi Research Center, Medical University of Graz, 8010 Graz, Austria
5
Department of Cardiac Surgery, University Hospital Basel, 4031 Basel, Switzerland
*
Author to whom correspondence should be addressed.
Diagnostics 2020, 10(4), 240; https://doi.org/10.3390/diagnostics10040240
Received: 16 March 2020 / Revised: 10 April 2020 / Accepted: 16 April 2020 / Published: 21 April 2020
(This article belongs to the Special Issue Animal Models for Studying and Screening Human Diseases)
Identification of microRNAs (miRNA) associated with cardiopulmonary bypass, cardiac arrest and subsequent myocardial ischemia/reperfusion may unravel novel therapeutic targets and biomarkers. The primary aim of the present study was to investigate the effects of cardiopulmonary bypass and temperature of cardioplegic arrest on myocardial miRNA profile in pigs’ left ventricular tissue. We employed next-generation sequencing to analyse miRNA profiles in the following groups: (1) hearts were arrested with antegrade warm St Thomas Hospital No. 2 (STH2) cardioplegia (n = 5; STH2-warm, 37 °C) and (2) cold STH2 (n = 6; STH2-cold, 4 °C) cardioplegia. Sixty min of ischemia was followed by 60 min of on-pump reperfusion with an additional 90 min of off-pump reperfusion. In addition, two groups without cardiac arrest (off-pump and on-pump group; n = 3, respectively) served as additional controls. STH2-warm and STH2-cold cardioplegia revealed no hemodynamic differences. In contrast, coronary venous creatine kinase-myocardial band (CK-MB) levels were significantly lower in pigs receiving STH2-warm cardioplegia (p < 0.05). Principal component analysis revealed that cardiopulmonary bypass and cardioplegic arrest markedly affected miRNAs in left ventricular tissue. Accordingly, ssc-miR-122, ssc-miR-10a-5p, ssc-miR-193a-3p, ssc-miR-499-3p, ssc-miR-374a-5p, ssc-miR-345-5p, ssc-miR-142-3p, ssc-miR-424-5p, ssc-miR-545-3p, ssc-miR-30b-5p, ssc-miR-145-5p, ssc-miR-374b-5p and ssc-miR-139-3p were differently regulated by cardiopulmonary bypass (false discovery rate (FDR) < 0.05 versus off-pump group). However, only ssc-miR-451 was differently expressed between STH2-warm and STH2-cold (FDR < 0.05). These data demonstrate for the first time that cardiopulmonary bypass and temperature of cardioplegic solution affected the expression of miRNAs in left ventricular tissue. In conclusion, specific miRNAs are potential therapeutic targets for limiting ischemia-reperfusion injury in patients undergoing cardiac surgery. View Full-Text
Keywords: microRNA; ischemia/reperfusion; cardiopulmonary bypass; cardioprotection microRNA; ischemia/reperfusion; cardiopulmonary bypass; cardioprotection
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MDPI and ACS Style

Kiss, A.; Heber, S.; Kramer, A.-M.; Hackl, M.; Skalicky, S.; Hallström, S.; Podesser, B.K.; Santer, D. MicroRNA Expression Profile Changes after Cardiopulmonary Bypass and Ischemia/Reperfusion-Injury in a Porcine Model of Cardioplegic Arrest. Diagnostics 2020, 10, 240. https://doi.org/10.3390/diagnostics10040240

AMA Style

Kiss A, Heber S, Kramer A-M, Hackl M, Skalicky S, Hallström S, Podesser BK, Santer D. MicroRNA Expression Profile Changes after Cardiopulmonary Bypass and Ischemia/Reperfusion-Injury in a Porcine Model of Cardioplegic Arrest. Diagnostics. 2020; 10(4):240. https://doi.org/10.3390/diagnostics10040240

Chicago/Turabian Style

Kiss, Attila; Heber, Stefan; Kramer, Anne-Margarethe; Hackl, Matthias; Skalicky, Susanna; Hallström, Seth; Podesser, Bruno K.; Santer, David. 2020. "MicroRNA Expression Profile Changes after Cardiopulmonary Bypass and Ischemia/Reperfusion-Injury in a Porcine Model of Cardioplegic Arrest" Diagnostics 10, no. 4: 240. https://doi.org/10.3390/diagnostics10040240

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