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Open AccessArticle

Rapid Multiplex Real-Time PCR Method for the Detection and Quantification of Selected Cariogenic and Periodontal Bacteria

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Department of Biochemistry, Faculty of Science, Masaryk University, Kotlarska 2, 611 37 Brno, Czech Republic
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Department of Pathophysiology, Faculty of Medicine, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic
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Clinic of Stomatology, Institution Shared with St. Anne’s Faculty Hospital, Faculty of Medicine, Masaryk University, Pekarska 53, 656 91 Brno, Czech Republic
*
Author to whom correspondence should be addressed.
Diagnostics 2020, 10(1), 8; https://doi.org/10.3390/diagnostics10010008
Received: 2 December 2019 / Revised: 14 December 2019 / Accepted: 18 December 2019 / Published: 22 December 2019
(This article belongs to the Section Diagnostic Microbiology and Infectious Disease)
Dental caries and periodontal diseases are associated with a shift from symbiotic microbiota to dysbiosis. The aim of our study was to develop a rapid, sensitive, and economical method for the identification and quantification of selected cariogenic and periodontal oral bacteria. Original protocols were designed for three real-time multiplex PCR assays to detect and quantify the ratio of 10 bacterial species associated with dental caries (“cariogenic” complex) or periodontal diseases (red complex, orange complex, and Aggregatibacter actinomycetemcomitans). A total number of 60 samples from 30 children aged 2–6 years with severe early childhood caries and gingivitis were tested. In multiplex assays, the quantification of total bacterial (TB) content for cariogenic bacteria and red complex to eliminate differences in quantities caused by specimen collection was included. The mean counts for the TB load and that of ten evaluated specimens corresponded to previously published results. We found a significant difference between the microbial compositions obtained from the area of control and the affected teeth (p < 0.05). Based on this comprehensive microbiological examination, the risk of dental caries or periodontal inflammation may be determined. The test could also be used as a tool for behavioral intervention and thus prevention of the above-mentioned diseases. View Full-Text
Keywords: dental caries; periodontitis; dysbiosis; oral microbiome; risk factors determination dental caries; periodontitis; dysbiosis; oral microbiome; risk factors determination
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Lochman, J.; Zapletalova, M.; Poskerova, H.; Izakovicova Holla, L.; Borilova Linhartova, P. Rapid Multiplex Real-Time PCR Method for the Detection and Quantification of Selected Cariogenic and Periodontal Bacteria. Diagnostics 2020, 10, 8.

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